Registration Dossier
Registration Dossier
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EC number: 429-270-1 | CAS number: 136210-30-5
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
Aspartic acid, N,N’-[methylenebis(2-methyl-4,1-cyclohexanediyl)]bis-, 1,1',4,4'-tetraethyl ester was tested for toxicity towards algal growth according to the OECD-Guideline 201 yielding an ErC 50 (0 - 48 h) > 1.319 mg/L as no toxic effects were observed at testing concentrations up to the water solubility limit of the test item (Currenta, 2010b).
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 1.319 mg/L
Additional information
The study was terminated after 48 h, because concentrations of the test item markedly decreased over time and thus the 48 h value reflects exposure better than the 72 h values. The cell number increased well above 16 -fold over 48 h and all other validity criteria are met as well. Furthermore, terminating an algal test after 48 h is an option explicity covered by OECD TG 201 (2006) and EU method C.3 (2009).
Studies were performed with aspartic acid, N,N'-[methylenebis(2-methyl-4,1-cyclohexanediyl)]bis-, 1,1',4,4'-tetraethyl ester which is a structural analogue to aspartic acid, N,N'-(methylenedi-4,1-cyclohexanediyl)bis-, 1,1',4,4'-tetraethyl ester. Both substances are diethyl esters of aspartic acid linked to a dicyclohexylmethyldiamine moiety. The difference between these two substances is merely the presence of two methyl groups connected to the cyclohexane rings. This structural analogy was confirmed by the Member State responsible for the notification of both substances under the NONS regulation. The Member State decided that test results obtained for one substance can be transferred to the other substance and that testing of both substances is usually not required. This decision is in accordance with the grouping of substances and read-across approach in Annex XI, 1.5 of the REACH Regulation.
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