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EC number: 427-820-5 | CAS number: 2701-50-0 1,2-METHYLEN-4,6-DIENACETAT
Endpoint summary
Administrative data
Description of key information
Oral (Rat, equivalent to OECD TG 423): LD50 > 2000 mg/kg
Dermal (Rat, GLP, equivalent to OECD TG 402): LD50 > 2000 mg/kg
Key value for chemical safety assessment
Acute toxicity: via oral route
Link to relevant study records
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Jan-Feb 1994
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)
- Version / remarks:
- pre-guideline study
- GLP compliance:
- not specified
- Test type:
- acute toxic class method
- Limit test:
- yes
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Route of administration:
- oral: gavage
- Doses:
- 2000 mg/kg (application volume 10 ml/kg)
- No. of animals per sex per dose:
- 3
- Control animals:
- no
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- no mortalities
- Other findings:
- Scab formation at the throat in 1 male animal from day 9 to 14 accompanied by an autopsy finding (appearance of foamy content in the broncholi of the lung)
These findings are not considered as compound-related but as an accidental injury. - Interpretation of results:
- GHS criteria not met
- Conclusions:
- The test item is of low oral toxicity.
- Executive summary:
The single oral administration of the test substance (ZK 5960) to male and female rats at a dose of 2000 mg/kg was tolerated without any mortality. No compound-related clinical signs were observed and there were no macroscopic pathological signs.
The LD50 acute oral toxicity of 1,2-Methylen-4,6-dienacetat in rats is therefore above 2000 mg/kg body weight.
Reference
No animal died in the course of the study.
No compound-related clinical findings were observed. The body weight gain observed on day 7 and day 14 was within or slightly above the normal range for rats (M+F) of the age and strain which were routinely used in the study laboratory.
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed
- Dose descriptor:
- LD50
- Value:
- > 2 000 mg/kg bw
- Quality of whole database:
- Klimisch score 2
Acute toxicity: via inhalation route
Endpoint conclusion
- Endpoint conclusion:
- no study available
Acute toxicity: via dermal route
Link to relevant study records
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- May - June 1994
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Version / remarks:
- 1987
- Deviations:
- yes
- Remarks:
- 3 instead of 5 animals/sex used according to acute-toxic-class-method (OECD 423)
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- yes
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Duration of exposure:
- 24 hours
- Doses:
- 2000 mg/kg bw
- No. of animals per sex per dose:
- 3
- Control animals:
- no
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- none
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The test item is of low acute dermal toxicity.
- Executive summary:
The single dermal administration of the test substance (ZK 5690) to male and female rats at the limit dose of 2000 mg/kg was tolerated without any mortality. No compound-related findings were observed with regard to local and clinical observation, determination of body weight and necropsy examination.
The LD50 acute dermal toxicity of the test item in rats is therefore above 2000 mg/kg body weight.
No local intolerance reactions at the application sites were observed.
Reference
No mortalities occurred. No compound-related findings were observed with regard to local and clinical observation, determination of body weight and necropsy examination.
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed
- Dose descriptor:
- LD50
- Value:
- > 2 000 mg/kg bw
- Quality of whole database:
- Klimisch score 2
Additional information
Justification for classification or non-classification
Based on the study results a classification according to Directive 67/548/EEC and Regulation (EC) No. 1272/2008 (CLP) is not required.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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