(E)-5[(4-chlorophenyl)methylene]-2,2-dimethylcyclopentanone

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

131984-21-9

Cas Number:

131984-21-9

IUPAC Name:

131984-21-9

Details on test material:

- Name of test material (as cited in study report): RPA 405217(DMBCP)
- Analytical purity: 95.6 %

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Two stock solutions were prepared. For the stock solution preparation 44.64 g/l and 1.04 g/l respectively were dissolved in Tween 80. The tes concentrations were prepared separately by dissolving the stock solution.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Tween 80
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): 0.1 g/l

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: rainbow trouts
- Source: Trout farm, Fredelsloh, Moringen
- Length at study initiation (length definition, mean, range and SD): 3.9 - 5.5 cm (4.6 cm)
- Weight at study initiation (mean and range, SD): 0.517 - 1.090 g (average: 0.859 g)
- Feeding during test: none


ACCLIMATION
- Type and amount of food: Tetramin fish food, ad libitum
- Health during acclimation (any mortality observed): less than 5 % mortality

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

50-250 mg/l

Test temperature:

15 ± 1 °C

pH:

7.8 - 8.2

Dissolved oxygen:

81 - 108 %

Nominal and measured concentrations:

Nominal test concentrations [mg/l]:
0.10, 0.35, 1.29, 4.29, 15.01, 52.52, 183.83

Measured test concentrations [mg/l]:
<0.019 (control), <0.019 (solvent control), 0.0268 (0.096 nominal), 0.425 (1.18 nominal), 8.295 (14.35 nominal), 73.164 (175.74 nominal),

Details on test conditions:

TEST SYSTEM
- Test vessel: glass aquarium
- Fill volume: 17 l
- Renewal rate of test solution (frequency/flow rate): after 48 h renewal of test solution
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: <= 1 g fish/l

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: filtered tap water

OTHER TEST CONDITIONS
- Photoperiod: 12: 12 h day-night regime
- Light intensity: 1000-1300 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Symptoms of intoxication and mortality after 3, 6, 24, 48, 72 und 96 hours

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

1.93 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

7.83 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

90.08 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

Results with nominal test substance concentrations:

NOEC (96h): 4.29 mg/l

LC0 (96h): 15.01 mg/l

NOEC (96h): 183.83 mg/l