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EC number: 306-248-9 | CAS number: 96690-53-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Version / remarks:
- 2006
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Sunflower oil, ester with sorbitol
- EC Number:
- 306-248-9
- EC Name:
- Sunflower oil, ester with sorbitol
- Cas Number:
- 96690-53-8
- IUPAC Name:
- sunflower seed oil sorbitol esters
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- Test organism: Desmodesmus subspicatus CHODAT SAG 86.81 (formerly Scenedesmus subspicatus CHODAT)
Reason for the selection of the test system: According to the guideline Desmodesmus subspicatus CHODAT SAG 86.81 is suitable for this kind of study.
Origin: Sammlung von Algenkulturen (SAG), Pflanzenphysiologisches lnstitut der Universität Göttingen, Nikolausberger Weg 18, 37073 Göttingen, Germany
Cultivation at test facility: Fresh stocks were prepared every month on Z-Agar. Light intensity amounted 35-70 µE.m-2.s-1 for 24 h per day.
Culture medium: Nutrient medium Z according to LOTTGE et al. (1994).
SELF FLUORESCENCE: No self fluorescence has been found at 100 mg/L.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 21-23°C ; mean 22°C
- pH:
- 7.7-9.37
- Nominal and measured concentrations:
- nominal: 100 mg/l
- Details on test conditions:
- TEST METHOD: Static procedure
Exposure duration: 72 h
Replicates: Six replicates per limit concentration and control
Test container: Sterile Erlenmeyer flasks, volume: 250 ml, sealed with cotton wool plugs
Test volume: 100 ml
Dilution water: According to the guideline
Preculture: A three day old preculture was used as inoculum. Incubation was performed in 500 mL Erlenmeyer flasks with dilution water. For the start of the test the preculture was directly pipetted to the test and control replicates to receive an initial cell concentration of approximately 2 - 5 x 10^3 cells/mL in the replicates.
Application: Application was, carried out by adding appropriate volumes of the stock solution to the test replicates.
Temperature: Nominal range: 21 - 24 °C, controlled at± 2 °C
Agitation: Test containers were placed on a rotary shaker and oscillated at approximately 70 rpm.
Light intensity: 60 - 120 µE.m-2.s-1
Light regime: 24 h/d light - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- Cell density was measured daily via Chlorophyll-a- fluorescence, excitation at 436 nm, emission at 685 nm. Dilution water was used as background signal. The pH-value at the beginning of the test was measured out of one additional replicate of the limit concentration and control. At the end it was measured from one pool of all replicates of the limit concentration and control. The room temperature was measured continuously by a hygro-thermograph. Light intensity was measured prior to test start. Microscopic evaluation of the cells at the start and end of the incubation were carried out. The cells were checked for unusual cell shapes, colour differences, differences in chloroplast morphology, flocculation, adherence of algae to test containers and agglutination of alga cells.
Microscopic evaluation of the cells at start and end of the incubation period revealed no morphological abnormalities.
The environmental conditions (pH-value, room temperature, light intensity) were determined to be within the acceptable limits. - Results with reference substance (positive control):
- The toxicity of potassium dichromate to the unicellular freshwater green alga Desmodesmus subspicatus was determined over a period of 72 h from March 09 to 12, 2009.
Test concentrations: 0.25 - 0.50 - 1.0 mg/L
ErC50= 0.695 mg/l (95%-CI: 0.657- 0.729 mg/l)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50-values for specific growth rate inhibition (ErC50) and inhibition of yield (EyC50) after 72 h were > 100 mg/L.
- Executive summary:
The toxicity of the test item to the unicellular freshwater green alga Desmodesmus subspicatus was determined according to the principles of OECD 201 at DR.U.NOACK-LABORATORIEN in 31157 Sarstedt, Germany from April 24 to 27, 2009. The aim of the study was to assess the effects on growth rate and yield production over a period of 72 h in a limit test. The study was conducted under static conditions with an initial cell density of nominally 2 - 5 x 103 cells/ml. Six replicates were tested for the limit concentration of 100 mg/L and the control. Environmental conditions were determined to be within the acceptable limits. The concentration of the test item was analysed at test start via DOC-analysis. All effect levels are given based on the nominal concentration of the test item.
The EC50-values for specific growth rate inhibition (ErC50) and inhibition of yield (EyC50) after 72 h were > 100 mg/L.
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