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Physical & Chemical properties

Density

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Reference
Endpoint:
relative density
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From 05/02/2018 to 06/03/2018
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
EU Method A.3 (Relative Density)
Deviations:
no
Qualifier:
according to guideline
Guideline:
OECD Guideline 109 (Density of Liquids and Solids)
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Type of method:
pycnometer method
Key result
Type:
relative density
Density:
1.294
Temp.:
20 °C
Remarks on result:
other: mean of 1.284 and 1.305

Determining the Density of the Immersion Liquid

Mass of Empty Pycnometer (g) : 24.07

Volume of Pycnometer (ml) : 25.3

Mass water & Pycnometer (g) : 49.34

Density of Water (g/cm3) (ρW,t) : 0.998

Mass of IL & Pycnometer (g) : 48.45

Mass IL in Pycnometer (g) (m1) : 24.38

Calculated Density of IL (g/cm3) : 0.963

Density of IL (kg/m3) : 963

Equation 1 (Calculation for the density of water)

Mass of water / volume of pycnometer = Density of water

Equation 2 (Calculation for the density of immersion liquid)

ρIL = (m1/mW) x ρW,t

Where : m1 is the mass of the immersion liquid

mW is the mass of water

ρW,t is the density of water at temperature t

Calculated value for the density of immersion liquid = 963 kg.m-3 @ 20°C

Determining the density of the sample

 RUN NUMBER  1  2
 Mass of Empty Pycnometer (g)  24.07  24.07
 Mass of Sample & Pycnometer (g)  34.79  33.73
 Mass of Sample added to empty Pycnometer (g) ms  10.72  9.66
 Mass of Sample, Pycnometer & immersion liquid  51.13  50.98
 Mass of immersion liquid added (g) m2  16.34  17.25
 Calculated Density of Sample (g/cm3)  1.284  1.305
 Density of Sample (kg/m3)  1284  1305

Equation 3 (Calculation for the density of the sample)

ρS,t = ms ρIL / m1 – m2

Where:

ms is the mass, in grams, of the sample;

m1 is the mass, in grams, of immersion liquid required to fill the empty pycnometer;

m2 is the mass, in grams of immersion liquid added to fill the pycnometer containing the sample;

ρIL is the density of the immersion liquid (as determined by equation 2)

Conclusions:
The relative density of Saccharomyces cerevisiae, lysate, is 1.294 according to the EU Method A.3.
Executive summary:

The relative density of Saccharomyces cerevisiae, lysate was determined according to the EU Method A.3 and the OECD Guideline 109, in compliance with the GLP.

The test was performed at 20°C with a pycnometer of kown volume. The density is calculated from the difference in weight between the full and empty pycnometer and its known volume. Two samples were tested giving the density of 1284 and 1305 kg/m3 respectively and the mean of 1294 kg. m-3.

The relative density of Saccharomyces cerevisiae, lysate is 1.294.

The relative density study is classified as acceptable, and satisfies the guideline requirements of the EU method A.3 and OECD 109.

Description of key information

The relative density of Saccharomyces cerevisiae, lysate, is 1.294.

Key value for chemical safety assessment

Relative density at 20C:
1.294

Additional information

The relative density of Saccharomyces cerevisiae, lysate was determined according to the EU Method A.3 and the OECD Guideline 109, in compliance with the GLP.

The test was performed at 20°C with a pycnometer of kown volume. The density is calculated from the difference in weight between the full and empty pycnometer and its known volume. Two samples were tested giving the density of 1284 and 1305 kg/m3 respectively and the mean of 1294 kg. m-3.

The relative density of Saccharomyces cerevisiae, lysate is 1.294.