Saccharomyces cerevisiae, lysate

Explosiveness

Link to relevant study record(s)

Reference

Endpoint:

explosiveness, other

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 05/02/2018 to 06/03/2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method A.14 (Explosive properties)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Key result

Parameter:

thermal sensitivity - Koenen test: diameter of the orifice plate

Remarks on result:

not thermal sensitive if heated under defined confinement

Remarks:

No explosion with the 3 tests using 6 mm orifice diameter and the 3 tests using the 2 mm orifice diameter.

Key result

Parameter:

sensitiveness to impact: impact energy (J)

Remarks on result:

not sensitive to impact using an impact energy of 40 J

Remarks:

No reaction or decomposition observed for the 6 tests performed

Key result

Parameter:

sensitiveness to friction: friction load (N)

Remarks on result:

not sensitive to friction using a friction load of 360 N

Remarks:

No reaction or decomposition during the 6 tests

Full test results (KOENEN TUBE)

Mass ofSample(g)	OrificeDiameter(mm)	Time to First Ignition (s)	Explosion (X /Y)*
16.7	6	31	X
16.7	6	26	X
16.7	6	29	X
16.7	2	26	X
16.7	2	22	X
16.7	2	21	X

* ‘Y’ denotes 3 or more fragments.

‘X’ denotes fewer than 3 fragments.

 

Full test results (BAM FALLHAMMER)

Mass (kg)	Height (m)	Energy (J)	Test number and results*(X /Y)						Comments
			1	2	3	4	5	6
10	0.4	40	X	X	X	X	X	X	No reaction observed

* ‘Y’ denotes an explosion (includes crackling, sparking, inflammation).

‘X’ denotes no reaction or decomposition (change incolour).

 

Full test results (BAM FRICTION)

Mass (kg)	Load (N)	Test number and results*(X /Y)						Comments
		1	2	3	4	5	6
8.34	360	X	X	X	X	X	X	No reaction observed

* ‘Y’ denotes an explosion (includes crackling, sparking, inflammation).

‘X’ denotes no reaction or decomposition (change incolour).

 

Interpretation of results:

GHS criteria not met

Conclusions:

Saccharomyces Cerevisiae, Lysate was not explosive when exposed to thermal or mechanical stress (shock and friction) under the test conditions.

Executive summary:

The determination of the explosive properties of Saccharomyces Cerevisiae, lysate was performed in accordance with EU method A.14 (Explosive properties) and in compliance with GLP.

The test substance was exposed to thermal and mechanical stress (shock and friction).

For the thermal sensitivity, a series of three tests with a 6.0 mm diameter orifice plate and a series of three tests with a 2.0 mm diameter orifice plate were performed on the sample using Koenen tube apparatus. The sample did not exhibit an explosion during any of the tests. The test result is therefore considered Negative (-) and the test item is not thermally sensitive.

A series of six tests was performed dropping the 10 kg mass from 0,4 m (40 J) on the sample using Bam Fallhammer apparatus for the sensitivity to shock. The sample was observed to exhibit no reaction at 40 J. The test result is therefore considered Negative (-) and the test item is not mechanical sensitive with respect to the shock.

To assess the sensitivity to the friction, a series of six tests was performed with a load of 360 N on the sample using Bam Friction apparatus. The sample was observed to exhibit no reaction at 360 N. The test result is therefore considered Negative (-) and the test item is not mechanical sensitive with respect to the friction.

In conclusion, Saccharomyces Cerevisiae, lysate was not explosive when exposed to thermal and mechanical stress (shock and friction) under the test conditions.

This explosive property study is classified as acceptable, and satisfies the guideline requirements of the EU method A.14.

Description of key information

Saccharomyces Cerevisiae, Lysate was not explosive when exposed to thermal or mechanical stress (shock and friction) under the test conditions of EU method A.14.

Key value for chemical safety assessment

Explosiveness:

non explosive

Additional information

The determination of the explosive properties of Saccharomyces Cerevisiae, lysate was performed in accordance with EU method A.14 (Explosive properties) and in compliance with GLP.

The test substance was exposed to thermal and mechanical stress (shock and friction).

For the thermal sensitivity, a series of three tests with a 6.0 mm diameter orifice plate and a series of three tests with a 2.0 mm diameter orifice plate were performed on the sample using Koenen tube apparatus. The sample did not exhibit an explosion during any of the tests. The test result is therefore considered Negative (-) and the test item is not thermally sensitive.

A series of six tests was performed dropping the 10 kg mass from 0,4 m (40 J) on the sample using Bam Fallhammer apparatus for the sensitivity to shock. The sample was observed to exhibit no reaction at 40 J. The test result is therefore considered Negative (-) and the test item is not mechanical sensitive with respect to the shock.

To assess the sensitivity to the friction, a series of six tests was performed with a load of 360 N on the sample using Bam Friction apparatus. The sample was observed to exhibit no reaction at 360 N. The test result is therefore considered Negative (-) and the test item is not mechanical sensitive with respect to the friction.

In conclusion, Saccharomyces Cerevisiae, lysate was not explosive when exposed to thermal and mechanical stress (shock and friction) under the test conditions.

Justification for classification or non-classification

The test substance was exposed to thermal and mechanical stress (shock and friction) under the consitions of the EU Method A.14 and it did not exhibit an explosion during any of these tests.

The test item is not thermally sensitive, it is not mechanical sensitive with respect to the shock and it is not mechanical sensitive with respect to the friction.

In conclusion, Saccharomyces Cerevisiae, lysate was not explosive when exposed to thermal and mechanical stress (shock and friction) under the test conditions.