β-Alanine, N-(2-carboxyethyl)-, N-coco alkyl derivs., disodium salts

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Testing appears to have been performed well and in accordance with guidelines. However, the work does not appear to be to GLP and the report lacks some key information. However, the results are backed up by other earlier work (supporting studies)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Report date:

2005

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

β-Alanine, N-(2-carboxyethyl)-, N-coco alkyl derivs., disodium salts

EC Number:

290-476-8

EC Name:

β-Alanine, N-(2-carboxyethyl)-, N-coco alkyl derivs., disodium salts

Cas Number:

90170-43-7

IUPAC Name:

β-Alanine, N-(2-carboxyethyl)-, N-coco alkyl derivs., disodium salts

Details on test material:

Sample tested was commercial grade material with approximately 35% active, containing a measured 22.6% w/w C.
Dosing levels took this into account

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Duration of test (contact time):

28 d

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (CO2 evolution)

Value:

ca. 70

Sampling time:

9 d

% Degradation 2

Parameter:

% degradation (CO2 evolution)

Value:

ca. 96

Sampling time:

28 d

Details on results:

Two concentrations tested, but lower concentration (approx 10 mg/l C) gave a % degradation over over 100% and has been disregarded.
The higher concentration of approximately 30 mg/l C resulted in a plateau of about 100% degradation after day 22. The 10 day window was achieved.
The positive control group reached a plateau of about 80% after 22 days, but failed to reach the 60% in 10 day criteria.

BOD5 / COD results

Results with reference substance:

The positive control group reached a plateau of about 80% after 22 days, but failed to reach the 60% in 10 day criteria.

Applicant's summary and conclusion

Validity criteria fulfilled:

no

Interpretation of results:

readily biodegradable

Executive summary:

There are obvious flaws in the testing, such as exceeding 100% for one of the concentrations tested and the poor performance of the positive control. However, earlier studies (albeit with less detail on methods and limited reporting) and estimations based on modelling and considering the class of substance suggests that the the substance is readily biodegradable.