Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 287-007-4 | CAS number: 85408-46-4
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 984
- Report date:
- 1988
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- April 1984
- Deviations:
- yes
- Remarks:
- Instead of a centrifuged sludge a settled sludge was used.
- GLP compliance:
- no
- Remarks:
- However, Quality Assurance Statement available
Test material
- Reference substance name:
- Amines, C12-14-tert-alkyl, bis[2-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]benzoato(2-)]chromate(1-)
- EC Number:
- 287-007-4
- EC Name:
- Amines, C12-14-tert-alkyl, bis[2-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]benzoato(2-)]chromate(1-)
- Cas Number:
- 85408-46-4
- Molecular formula:
- C17H12N4O3 . C17H12N4O3 . Cr. C12H28N to C14H32N
- IUPAC Name:
- Amines, C12-14-tert-alkyl, bis[2-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]benzoato(2-)]chromate(1-)
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- not applicable
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- other: activated sludge, domestic, non-adapted
- Details on inoculum:
- - Preparation of inoculum for exposure: The preparation was carried out according to the method described in the guideline, except the settling instead of centrifuging.
- Initial biomass concentration: the sludge concentrations in the test bottles was 1.6 g/L
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- none
Test conditions
- Hardness:
- no data
- Test temperature:
- 20+/- 2 °C
- pH:
- no data
- Dissolved oxygen:
- no data
- Salinity:
- no data
- Nominal and measured concentrations:
- Reference substance : 32, 10 and 3.2 mg/L (nominal)
Test substance: 100, 32, 10, 3.2 ans 1 mg/L (nominal) - Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL BOD flasks with gas inlet
- Biomass loading rate: the sludge concentration in the test bottles was 1.6 g/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated drinking water
TEST CONCENTRATIONS
- Test concentrations: Reference substance: 32, 10 and 3.2 mg/L (nominal), test substance: 100, 32, 10, 3.2, 1 mg/L - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Diclorophenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: IC80
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: IC20
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- - Relevant effect levels: IC50 = 11mg/L
- Reported statistics and error estimates:
- no data
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The inhibition of the degradation activity of activated sludge is not anticipated when introduced to biological treatment plants in appropriate low concentrations.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
