Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 281-876-3 | CAS number: 84051-87-6
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Experimental test result performed according to the guideline.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Principles of method if other than guideline:
- Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).
- GLP compliance:
- not specified
- Analytical monitoring:
- not required
- Remarks:
- Stable in recommended storage conditions
- Vehicle:
- not specified
- Details on test solutions:
- The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 1 g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous 1 hour stirring for achieving test concentrations of 6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/L,respectively
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source: Unique Aqua Fish Corner
- Length at study initiation (length definition, mean, range and SD): average 1.7 cm
- Weight at study initiation (mean and range, SD): average 0.059 g
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: 16 hours light and 8 hours dark
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day - Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 96 h
- Hardness:
- 155.5 mg of CaCO3
- Test temperature:
- 23.4 °C
- pH:
- 7.3
- Dissolved oxygen:
- 7.0 mg/l
- Nominal and measured concentrations:
- 6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours dark
- Reference substance (positive control):
- not specified
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 12.5 - < 25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: LC50 (96 hours) Experimental = >12.5 mg/L but <25 mg/L
- Sublethal observations / clinical signs:
8.1. Mortality and symptoms
Nominal
Number of
Mortality (cumulated) after
Concentration fish
3 h
6 h
24h
48h
72h
96h
(mg/l)
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
-
-
-
-
-
-
-
-
-
-
-
-
6.25
4
4
-
-
-
-
-
-
-
-
-
-
-
-
12.5
4
4
-
-
-
-
-
-
-
-
-
-
-
-
25
4
4
-
-
-
-
-
-
-
-
1
1
4
4
50
4
4
-
-
-
-
-
-
2
2
4
4
4
4
100
4
4
-
-
4
4
4
4
4
4
4
4
4
4
Visible Symptoms:
Nominal
Concentration
(mg/l)
Number of
fish
symptom (cumulated) after
3h
6h
24h
48h
72h
96h
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
6.25
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
12.5
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
25
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS3
NS3
-
-
50
4
4
NS4
NS4
NS4
NS4
NS4
NS2
NS2
NS2
-
-
-
-
100
4
4
M4
M4
-
-
-
-
-
-
-
-
-
-
Explanation of symptoms:
A: apathy
N: Narcotic-like state
C: Swimming near water surface
M: Slow movements compared to control
NS: No abnormal symptoms
S: Surfacing behaviour
L: Loss of equilibrium
SM: Shimming movement
H1: Hyperactivity
H2: Hypo activity
B: Bending behavior
F: Fin rot
IS: Invert Swimming
SB: Swimming behaviour
RF: Respiratory Function
P: Pigmentation
8.2. pH and oxygen content:
Number of
pH readings after
Nominal
fish
24h
48h
72h
96h
Concentration
V1
V2
V1
V2
V1
V2
V1
V2
(mg/l)
Control
4
4
7.3
7.3
7.5
7.5
7.6
7.6
7.7
7.7
6.25
4
4
7.5
7.5
7.6
7.6
7.7
7.7
7.8
7.8
12.5
4
4
7.5
7.6
7.7
7.7
7.8
7.8
7.9
7.9
25
4
4
7.6
7.6
7.8
7.8
7.9
7.9
-
-
50
4
4
7.7
7.7
7.9
7.9
-
-
-
-
100
4
4
-
-
-
-
-
-
-
-
Nominal
Number of
Oxygen content (mg/L) after
Concentrationfish
24h
48h
72h
96h
(mg/l)
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
7.0
7.0
6.9
6.9
6.8
6.8
6.7
6.7
6.25
4
4
6.9
6.9
6.8
6.8
6.7
6.7
6.6
6.6
12.5
4
4
6.9
6.9
6.8
6.8
6.7
6.7
6.6
6.6
25
4
4
6.9
6.9
6.8
6.8
6.7
6.7
-
-
50
4
4
6.9
6.9
6.8
6.8
-
-
-
-
100
4
4
-
-
-
-
-
-
-
-
8.3. Test temperature
Nominal
Number of
Temperature (°C) after
Concentrationfish
24h
48h
72h
96h
(mg/l)
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
22.5
22.5
23.1
23.1
23.2
23.2
23.1
23.1
6.25
4
4
22.5
22.5
23.1
23.1
23.2
23.2
23.1
23.1
12.5
4
4
22.5
22.5
23.1
23.1
23.2
23.2
23.1
23.1
25
4
4
22.5 22.5
23.1
23.1
23.2
23.2
-
-
50
4
4
22.5
22.5
23.1
23.1
-
-
-
-
100
4
4
-
-
-
-
-
-
-
-
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on nominal concentrations, experimental median lethal Concentrations of test mayterial on Zebra fish(Danio rerio)was found to be >12.5 mg/L but <25 mg/L
- Executive summary:
Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 1 g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous 1 hour stirring for achieving test concentrations of 6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/L,respectively. This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio rerio were exposed to these concentration for 96 hours.
Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to various nominal test concentrations, LC50 was determine to be >12.5 but <25 mg/l.100% mortality was observed in the concentration 25 mg/l . Based on the LC50, it can be consider that the chemical was toxic and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.
Reference
Description of key information
Short term toxicity to fish:
Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 1 g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous 1 hour stirring for achieving test concentrations of 6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/L,respectively. This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours.
Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to various nominal test concentrations, LC50 was determine to be >12.5 but <25 mg/l.100% mortality was observed in the concentration 25 mg/l . Based on the LC50, it can be consider that the chemical was toxic and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 12.5 mg/L
Additional information
Short term toxicity to fish:
Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 1 g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous 1 hour stirring for achieving test concentrations of 6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/L,respectively. This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours.
Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to various nominal test concentrations, LC50 was determine to be >12.5 but <25 mg/l.100% mortality was observed in the concentration 25 mg/l . Based on the LC50, it can be consider that the chemical was toxic and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
