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Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

Currently viewing:

Administrative data

Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data is from peer reviewed journal

Data source

Reference
Reference Type:
publication
Title:
To evalaute toxicity of test material on miroorganisms
Author:
FUMIYO SAKO et al
Year:
1977
Bibliographic source:
TOXICOLOGY AND APPLIED PHARMACOLOGY 1977

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: refer below principle
Principles of method if other than guideline:
The effect of test material on leucine aminopeptidase, acid phosphatase, and y-glutamyl transpeptidase activity in P. caudatum was studied in order to investigate the mechanism of toxicity.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Dihydrogen (ethyl)[4-[4-[ethyl(3-sulphonatobenzyl)amino](4-hydroxy-2-sulphonatobenzhydrylidene]cyclohexa-2,5-dien-1-ylidene](3-sulphonatobenzyl)ammonium, disodium salt
EC Number:
219-091-5
EC Name:
Dihydrogen (ethyl)[4-[4-[ethyl(3-sulphonatobenzyl)amino](4-hydroxy-2-sulphonatobenzhydrylidene]cyclohexa-2,5-dien-1-ylidene](3-sulphonatobenzyl)ammonium, disodium salt
Cas Number:
2353-45-9
Molecular formula:
C37H36N2O10S3.2Na
IUPAC Name:
disodium 2-({4-[ethyl(3-sulfonatobenzyl)amino]phenyl}{4-[ethyl(3-sulfonatobenzyl)iminio]cyclohexa-2,...
Details on test material:
- Name of test material (as cited in study report):Fast green FCF
- Molecular formula (if other than submission substance):C37-H36-N2-O10-S3.2Na
- Molecular weight (if other than submission substance):808.8576
- Smiles notation (if other than submission substance):C(=C1\C=C\C(=[N+](\Cc2cc(ccc2)S(=O)(=O)[O-])CC)C=C1)(/c1c(cc(cc1)O)S(=O)(=O)O)c1ccc(cc1)N(Cc1cc(ccc1)S(=O)(=O)O)CC.[Na].[Na]
- InChl (if other than submission substance):1S/C37H36N2O10S3.2Na/c1-3-38(24-26-7-5-9-33(21-26)50(41,42)43)30-15-11-28(12-16-30)37(35-20-19-32(40)23-36(35)52(47,48)49)29-13-17-31(18-14-29)39(4-2)25-27-8-6-10-34(22-27)51(44,45)46;;/h5-23H,3-4,24-25H2,1-2H3,(H3,41,42,43,44,45,46,47,48,49);;/q;2*+1/p-2
- Substance type: Organic
- Physical state: Solid

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:Food dye of various concentrations were put in a hollow slide glass, and an equal volume of 0.04 M phosphate buffer, pH 7.0, was added.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant):phosphate buffer
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)):0.1 M

Test organisms

Test organisms (species):
Paramaecium caudatum
Details on inoculum:
- Laboratory culture: PC was maintained at 22°C on 0.15 % dried lettuce infusion and fed with Aerobacter aerogenes.
- Preparation of inoculum for exposure:One week after inoculation, PC culture was filtered through two thicknesses of muslin. This was centrifuged at 8000 g for 10 min and the packed cells were then washed in a solution of 0.12 M NaCl and resedimented by centrifugation at 8000 g for 10 min. The pellet of cells was then sonicated in 0.1 M sodium phosphate buffer, pH 7.2, for 20 sec. The resulting sonicate was centrifuged at 8000 g for 10 min, and the supernatant fraction was used as the crude enzyme extract. The centrifugation and the subsequent procedures were carried out at 0-4°C.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
17.28 min
Remarks on exposure duration:
study reported in 1037 sec.

Test conditions

Hardness:
no data
Test temperature:
22 deg.C
pH:
7.2
Dissolved oxygen:
no data
Salinity:
no data
Nominal and measured concentrations:
0.1 and 1.0 % test concentration
Details on test conditions:
test material of 0.1 and 1.0 % concentrations were put in a hollow slide glass, and an equal volume of 0.04 M phosphate buffer, pH 7.0, was added. After 5 to 10 PC were added, their survival times were measured microscopically. Thirty to forty PC for each concentration were tested by the same method, and the mean survival time and the death rate were calculated. The survival time was defined as the time required until death was observed for each concentration. Death was assumed to have occurred when there was no movement. The death rate was defined as the percentage of deaths observed during 20 min.
Reference substance (positive control):
not specified

Results and discussion

Effect concentrations
Duration:
17.28 min
Dose descriptor:
LOEC
Effect conc.:
0.1 other: %
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Remarks on result:
other: 36.7% mortality observed of test organism i.e Paramecium caudatum after of 17.28 min.which is not consider to be very toxic.
Reported statistics and error estimates:
The correlation coefficient between the inhibitory activity of these food dye on LAP and the death rate showed r values of 0.7952 (p < 0.05).

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
The effect of test material on the survival time of Paramaecium caudatum was measured microscopically for 20 min. Concentration of test material in the bathing fluid was 0.1 or 1 .0%.
During the experiment the 36.7% mortality observed of test organism i.e Paramecium caudatum after of 17.28 min.which is not consider to be very toxic.
Executive summary:

The effect of test material on leucine aminopeptidase, acid phosphatase, and y-glutamyl transpeptidase activity in P. caudatum was studied in order to investigate the mechanism of toxicity.

The effect of test material on the survival time of Paramaecium caudatum was measured microscopically for 20 min. Concentration of test material in the bathing fluid was 0.1 or 1 .0%.

During the experiment, 36.7% mortality observed of test organism i.e Paramecium caudatum after exposure of 17.28 min. Thus on that basis chemical was not consider to be very toxic.