3,6-bis(diethylamino)-9-[2-(methoxycarbonyl)phenyl]xanthylium tetrachlorozincate

Short-term toxicity to fish

Description of key information

This study was designed to assess the toxic effects of the test compound on the Zebra fish (Danio rerio). A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. The test substance was highly soluble in water. Therefore, the test solution was prepared by dissolving 1g of the test substance in 1 liters of potable water (passed through reverse osmosis system) for achieving test concentrations of 1g/L respectively. After that kept it for 1 hr. stirring, it was analytical detection and then used for experimental addition. Test conducted at a different concentrations of 0.98mg/L,1.48mg/L,2.22mg/L,3.33mg/L,5mg/L. A 96 h study the fishes were moving slowly as compared to control. No mortalities were observed in the control aquaria. Based on the mortality of test fishes because of chemical 3,6-bis(diethyl amino)-9-[2-(methoxycarbonyl)phenyl]xanthylium tetrachlorozincate exposure for 96 hrs the LC50 was observed at 0.98 mg/l. LC100 (96 hours) (lowest loading at which 100% mortality was observed) was determine at 1.48 mg/L. It can be concluded from the value that the 3,6-bis(diethyl amino)-9-[2-(methoxycarbonyl)phenyl]xanthylium tetrachlorozincate is toxic to the fish and can be considered as “aquatic acute 1” as per the classification criteria for aquatic environment.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

0.98 mg/L

Additional information

Various experimental studies for target compound 3,6-bis(diethyl amino)-9-[2-(methoxycarbonyl) phenyl]xanthylium tetrachlorozincate (CAS 73398-89-7) and supporting studies for its closest read across substance on the basis of structural and functional similarity were reviewed for the toxicity on the fish end point which are summarized as below: 

 

In the first key study from experimental report 2016 was designed to assess the toxic effects of the test compound on the Zebra fish (Danio rerio). A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. The test substance was highly soluble in water. Therefore, the test solution was prepared by dissolving 1g of the test substance in 1 liters of potable water (passed through reverse osmosis system) for achieving test concentrations of 1g/L respectively. After that kept it for 1 hr. stirring, it was analytical detection and then used for experimental addition. Test conducted at a different concentrations of 0.98mg/L,1.48mg/L,2.22mg/L,3.33mg/L,5mg/L. A 96 h study the fishes were moving slowly as compared to control. No mortalities were observed in the control aquaria. Based on the mortality of test fishes because of chemical 3,6-bis(diethyl amino)-9-[2-(methoxycarbonyl)phenyl]xanthylium tetrachlorozincate exposure for 96 hrs the LC50 was observed at 0.98 mg/l. LC100 (96 hours) (lowest loading at which 100% mortality was observed) was determine at 1.48 mg/L. It can be concluded from the value that the 3,6-bis(diethyl amino)-9-[2-(methoxycarbonyl) phenyl]xanthylium tetrachlorozincate is toxic to the fish and can be considered as “aquatic acute 1” as per the classification criteria for aquatic environment.

 

First study was supported by another experimental data for structurally and functionally similar read across chemical from report 2016. This study was designed to assess the toxic effects of the test chemical on the Zebra fish (Danio rerio). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 500 mg of the test substance in 500 ml of potable water (passed through reverse osmosis system) with continuous 1 hr stirring. After stirring stock was analytically detected and the solubility was found 672.34 mg/L, on the basis of this value following test concentration was prepared 0.1562mg/L, 0.3125mg/L, 0.625mg/L, 1.25mg/L,2.5mg/L ,respectively. Study conducted under the static system for 96 hrs. Test animal was collected from Anytime Pet, Jariphataka, Nagpur. 8 fishes exposed to the test concentration. Aeration in test vessels was provided 1 day before the start of experiment. After the exposure of chemical (mortality, visible symptoms, pH, Temperature, dissolved oxygen content) were recorded after 24 hours, 48 hours, 72 hours and 96 hours of the start of the experiment. Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] of chemical on Zebra fish (Danio rerio) was determined to be < 0.1562 mg/L by observing the mortality. Thus based on LC50 it can be concluded that the chemical was toxic and classified as aquatic acute 1 / chronic 1 as per the CLP criteria.

 

Thus based on the above studies, it can be concluded that the chemical was toxic and classified as aquatic acute 1 / chronic 2 as per the CLP classification criteria.