Trisodium bis[3-[[1-(anilinocarbonyl)-2-oxopropyl]azo]-5-chloro-2-hydroxybenzenesulphonato(3-)]cobaltate(3-)

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: screening test, other

Remarks:

BOD5/COD

Type of information:

other: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Study period:

June 19,1979

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Short summary

Remarks:

The test was conducted by means of Read Across approach. The reliability of the source study report is 4. Further information was attached at section 13

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))

Deviations:

not specified

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method C.6 (Degradation: Chemical Oxygen Demand)

Deviations:

not specified

GLP compliance:

no

Remarks:

Pre GLP.

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Duration of test (contact time):

ca. 5 d

Initial conc.:

ca. 500 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Parameter:

% degradation (O2 consumption)

Remarks on result:

not measured/tested

Parameter:

BOD5

Value:

ca. 0 mg O2/g test mat.

Parameter:

COD

Value:

ca. 994.36 mg O2/g test mat.

Parameter:

BOD5*100/COD

Value:

ca. 0 mg O2/g test mat.

Biochemical oxygen demand or BOD is the amount of dissolved oxygen needed by aerobic biological organisms in a body of water to break down organic material present in a given water sample at certain temperature over a specific time period. The term also refers to a chemical procedure for determining this amount. This is not a precise quantitative test, although it is widely used as an indication of the organic quality of water, therefore is not be used as quantitative date about ready biodegradation.

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

BOD5 = 0 mg/g O2
COD = 994.36 mg/g O2
BOD5/COD = 0

Executive summary:

BOD5 = 0 mg/g O2

COD = 994.36 mg/g O2

BOD5/COD = 0

Reference 2

Endpoint:

biodegradation in water: screening test, other

Remarks:

BOD5/COD

Type of information:

other: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Study period:

October 31, 1977

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Short summary

Remarks:

The test was conducted by means of Read Across approach. The reliability of the source study report is 4. Further information was attached at section 13

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))

Deviations:

not specified

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method C.6 (Degradation: Chemical Oxygen Demand)

Deviations:

not specified

GLP compliance:

no

Remarks:

Pre GLP.

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Duration of test (contact time):

ca. 5 d

Initial conc.:

ca. 50 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Parameter:

% degradation (O2 consumption)

Remarks on result:

not measured/tested

Parameter:

BOD5

Value:

ca. 108 mg O2/g test mat.

Parameter:

COD

Value:

ca. 919.94 mg O2/g test mat.

Parameter:

BOD5*100/COD

Value:

ca. 12 mg O2/g test mat.

Biochemical oxygen demand or BOD is the amount of dissolved oxygen needed by aerobic biological organisms in a body of water to break down organic material present in a given water sample at certain temperature over a specific time period. The term also refers to a chemical procedure for determining this amount. This is not a precise quantitative test, although it is widely used as an indication of the organic quality of water, therefore is not be used as quantitative date about ready biodegradation.

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

BOD5=108 mg/g O2
COD=919.94 mg/g O2
BOD5/COD=0.12

Executive summary:

BOD5=108 mg/g O2

COD=919.94 mg/g O2

BOD5/COD=0.12

Reference 3

Endpoint:

biodegradation in water: screening test, other

Remarks:

BOD5/COD

Type of information:

other: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Study period:

November 7,1978

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

abstract

Remarks:

The test was conducted by means of Read Across approach. The reliability of the source study report is 4. Further information was attached at section 13

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))

Deviations:

not specified

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method C.6 (Degradation: Chemical Oxygen Demand)

Deviations:

not specified

GLP compliance:

no

Remarks:

Pre GLP.

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Duration of test (contact time):

ca. 5 d

Initial conc.:

ca. 50 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Parameter:

% degradation (O2 consumption)

Remarks on result:

not measured/tested

Parameter:

BOD5

Value:

ca. 116 mg O2/g test mat.

Parameter:

COD

Value:

ca. 992.29 mg O2/g test mat.

Parameter:

BOD5*100/COD

Value:

ca. 11.69 mg O2/g test mat.

Biochemical oxygen demand or BOD is the amount of dissolved oxygen needed by aerobic biological organisms in a body of water to break down organic material present in a given water sample at certain temperature over a specific time period. The term also refers to a chemical procedure for determining this amount. This is not a precise quantitative test, although it is widely used as an indication of the organic quality of water, therefore is not be used as quantitative date about ready biodegradation.

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

BOD5 = 116 mg/g O2
COD = 992.29 mg/g O2
BOD5/COD = 0.12

Executive summary:

BOD5 = 116 mg/g O2

COD = 992.29 mg/g O2

BOD5/COD = 0.12

Reference 4

Endpoint:

biodegradation in water: screening test, other

Remarks:

BOD5

Type of information:

other: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Study period:

From July 5 to 10, 1990

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

The test was conducted by means of Read Across approach. The reliability of the source study report is 1. Further information was attached at section 13

Qualifier:

according to guideline

Guideline:

other: EEC Directive 79-831, Annex V, Part C.8 , DEGRADATION BIOCHEMICAL OXYGEN DEMAND

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: according to DEV H-5 (a2) Deutsche Einheitsverfahren, 1966

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.9 (Biodegradation: Zahn-Wellens Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: German DIN-Norm 38'409, H-41, 1

Deviations:

no

GLP compliance:

yes

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge:mixed culture of bacteria (effluent of a Husman laboratory apparatus)

Duration of test (contact time):

ca. 5 d

Initial conc.:

ca. 1 mg/L

Based on:

test mat.

Initial conc.:

ca. 5 mg/L

Based on:

test mat.

Initial conc.:

ca. 10 mg/L

Based on:

test mat.

Initial conc.:

ca. 20 mg/L

Based on:

test mat.

Initial conc.:

ca. 50 mg/L

Based on:

test mat.

Initial conc.:

ca. 100 mg/L

Based on:

test mat.

Initial conc.:

ca. 200 mg/L

Based on:

test mat.

Initial conc.:

ca. 500 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Test temperature: 22 ± 2°C

TEST SYSTEM
- Culturing apparatus:
- Number of culture flask: 1
- Measuring equipment: O2 Determination with an Oxygensensitive-electrode: SYLAND

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes

Reference substance:

benzoic acid, sodium salt

Parameter:

% degradation (O2 consumption)

Value:

ca. 0

Sampling time:

5 d

Parameter:

BOD5

Value:

ca. 0 mg O2/g test mat.

Parameter:

COD

Value:

ca. 992 mg O2/g test mat.

Parameter:

BOD5*100/COD

Value:

0

Results with reference substance:

for 4 mg/l reference substance = 1050 mgO2/g
for 5 mg/l reference substance = 1060 mgO2/g

VALIDITY CRITERIA:

- Blank

B0D5: 0,5 or less mgO2/l

- Reference substance

B0D5: not less than 960 mgO2/l

(concentration 4 mg/l and 5 mg/l)

Test substance:

Test concentration mg/l	O2-Start	O2-End	Blank	BOD5 mgO2/g
1	8.9	8.4	0.2	300
5	8.9	8.5	0.2	40
10	8.9	8.5	0.2	20
20	8.9	8.3	0.2	20
50	8.9	8.4	0.2	6
100	8.9	8.4	0.2	3
200	8.9	8.4	0.2	2
500	8.8	8.3	0.2	1

Reference substance:

Test concentration mg/l	O2-Start	O2-End	Blank	BOD5 mgO2/g
4	8.8	4.4	0.2	1050
5	8.9	3.4	0.2	1060

The B0D5 of the test item can not be calculated because the measured end value "02 -End" is below 2 mgO2/l and

therefore it does not fulfil the validity criteria.

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

The BOD5 of the test substance, corrected by the blank control is: 0 mgO2/g, therefore BOD5/COD = 0

Executive summary:

The substance has been tested for ready biodegradation according to the EEC Directive 79-831, Annex V, Part. C.8 and according to DEV H-5 (a2) Deutsche Einheitsverfahren, 1966. The value of degradation of test item is below the limit of detection, therefore under test conditions no biodegradation it is observed.

Reference 5

Endpoint:

biodegradation in water: inherent biodegradability

Type of information:

other: read-across from supporting substance (structural analogue or surrogate)read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Study period:

From August 2 to September 12, 1990

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

The test was conducted by means of Read Across approach. The reliability of the source study report is 1. Further information was attached at section 13

Qualifier:

according to guideline

Guideline:

OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: ISO Method 8129 B

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, adapted

Details on inoculum:

- Source of inoculum/activated sludge: 1000 mg/l
- Laboratory culture: Sludge of a domestic sewage treatment plant (ARA-Rhein) collected on 14.08.90

Duration of test (contact time):

ca. 28 d

Initial conc.:

ca. 100 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium: According to the specification of the EEC L133 (pp.99-105) Volume 31 resp. OECD Method 302B.
- Test temperature:22 ± 3 °C
- Continuous darkness: no, indirect daylight

Reference substance:

diethylene glycol

Parameter:

% degradation (O2 consumption)

Value:

ca. 62.45

Sampling time:

28 d

Details on results:

Adsorption after 3 hours: 31.35 %

Results with reference substance:

Biodegradation corrected by the blank control and measured as DOC (mg/l) is: 99.6 % (12th day)

Biodegradation after 28 days:

TIME		Value of the substance DOC (mg/l)		Value of the blank DOC (mg/l)		Elimination (%)
		1° sample	2° sample	1° sample	2° sample	1° sample	2° sample
0	h	78.2	79.5	6	6	24.7	23.8
3	h	73.9	72.6	7.2	7.2	30.5	32.2
2	day	62	65.5	4.4	4.4	39.9	36.7
5	day	59.9	59.1	7.4	7.4	45.3	46.4
7	day	58.9	58.1	5.6	5.6	44.4	45.6
9	day	59.1	58.3	8.1	8.1	46.8	48
12	day	56.9	56.8	8.9	8.9	50	50.4
14	day	58.1	55.6	7.5	7.5	47.2	50.2
16	day	54.3	52.9	11	11	54.9	56.6
19	day	51.6	48.8	10.9	10.9	57.6	60.7
21	day	53.2	48.4	12.1	12.1	57.1	62.4
23	day	49.8	49.5	10.1	10.1	58.6	59.2
26	day	48.7	46.7	9.5	9.5	59.1	61.5
28	day	47.7	46.9	11.2	11 .2	61.9	63.0

Reference substance:

TIME		Value of the substance DOC (mg/l)	Value of the blank DOC (mg/l)	Elimination (%)
0	h	107.6	6	0
3	h	105.9	7.2	0.3
2	day	107.2	4.4	0
5	day	15.8	7.4	91 .5
6	day	4.9	5.3	100
7	day	5.7	5.6	99.9
8	day	7.9	6.3	98.4
9	day	8.4	8.1	99.7
12	day	9.3	8.9	99.6

Validity criteria:

The test is considered valid if the procedural control shows the removal of the reference compound by at least 70 % within 14d and if the removal of DOC (or COD) in the test suspension took place relatively gradually over days or weeks, since this indicates biodegradation.

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

Elimination measured as DOC (mg/l) after 28 days corrected by the blank control is: 62.45 %
Adsorption after 3 hours = 31.35 %

Executive summary:

The substance has been tested for Inherent biodegradation according to the OECD 302B. The biodegradation after 28 days , corrected by the blank control, it is: 62.45 % (average of 2 tests running in parallel). Adsorption after 3 hours = 31.35 %.

Description of key information

Elimination = 62.45 %

Additional information

The substance is not readily biodegradable based on Expert Judgement and on the data on the similar substance.