Amines, C16-18 and C18-unsatd. alkyl

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

migrated information: read-across based on grouping of substances (category approach)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Amines, coco alkyl

EC Number:

262-977-1

EC Name:

Amines, coco alkyl

Cas Number:

61788-46-3

IUPAC Name:

61788-46-3

Details on test material:

- Name of test material (as cited in study report): Cocoalkylamine

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stock solution was prepared with natural river water. Dispersion carried out with: 30 min ultrasound at 40 °C

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Common name: Green algae
- Strain: Desmodesmus subspicatus CHODAT SAG 86.81
- Source: The algae culture was optained from the Institut of plant physiology, University of Göttingen, Germany;
- Method of cultivation: Fresh stock solutions were prepared every month on Z-agar. Light intensity amounted 35-70 µE/m²

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Hardness:

91.3 mg CO3/L

Test temperature:

23 ± 2 °C

pH:

8.08 - 8.15

Nominal and measured concentrations:

Nominal concentrations: control, 0.03, 0.06, 0.13, 0.25, 0.5 and 1 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, fill volume: 250 mL-Erlenmayer-flasks with 100 mL test volume
- Initial cells density: 9000-10000 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6


GROWTH MEDIUM
- Standard medium used: Yes


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: A natural river water of agricultural backround, middle reach of the river "Böhme", Lower-Saxony was used.
Test water parameter:
pH: 8.2
Conductivity [μS/cm]: 397
DOC [mg C/L]: 7.3
TOC [mg C/L]: 9.9
Ammonium-N [mg N/L]: 0.141
Nitrate-N [mg N/L]: 2.38
o-Phosphate-P [mg P/L]: 0.031
Total Phosphate [mg P/L]: 0.128
Suspended Matter [mg/L]: 17.4
Total Hardness [mg CO3/L]: 91.3
- Culture medium different from test medium: yes
- Intervals of water quality measurement: cell density was measured every 24 hours. Temperature was measured permanently and the pH value was determinated at the beginning and the end of the test.


OTHER TEST CONDITIONS
- Photoperiod: Permanent illumination (24h)
- Light intensity and quality: 60-120 µE/m²*s



EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: fluorimeter and microscopic evaluation

Reference substance (positive control):

yes

Remarks:

potassium dicromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

0.16 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

0.06 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 3

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

0.13 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 4

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

0.08 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 5

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

0.03 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 6

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

0.06 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Any other information on results incl. tables

After 72 h algae were transferred from the nominal dosage levels 0.13 -1.0 mg/L to fresh untreated medium and allowed to grow for further 5 -11 days under test conditions. The test item effect was observed to be reversible at these concentration levels (exept one of three replicates of the highest test concentration of 1 mg/L, where no growth could be detected with 11 d of incubation).

Table 1: Concentration of the test item:

Nominal test item conc. [mg/L]	Meas. conc. [mg/L]after 0 h	Meas. conc. [mg/L]after 3 d
20.00	16.1	12.9
1.00	(18.4)	< detection limit
Control	< detection limit	< detection limit

Applicant's summary and conclusion

Conclusions:

In this study Cocoalkylamine was found to inhibit the growth of the freshwater green alga Desmodesmus subspicatus at concentrations > 0.06 mg/L (biomass growth) and > 0.13 mg/L (growth rate). The EC50 values with 95 % confidence intervals for inhibition of biomass growth (EbC50) and specific grothw rate (ErC50) after 72h were 0.08 (0.07 - 0.09) and 0.16 (0.15 - 0.18) mg/L, respectively.
Inhibition effects were found to be reversible up to 1.0 mg/L (all effect levels are given as nominal concentrations)

Executive summary:

In this study Cocoalkylamine was found to inhibit the growth of the freshwater green alga Desmodesmus subspicatus at concentrations > 0.06 mg/L (biomass growth) and > 0.13 mg/L (growth rate). The EC50 values with 95 % confidence intervals for inhibition of biomass growth (EbC50) and specific grothw rate (ErC50) after 72h were 0.08 (0.07 - 0.09) and 0.16 (0.15 - 0.18) mg/L, respectively.