[29H,31H-phthalocyaninetrisulphonyl trichloridato(2-)-N29,N30,N31,N32]copper

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: According to OECD guideline and GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: Directive 92/69/EEC, part C.4-A

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

[29H,31H-phthalocyaninetrisulphonyl trichloridato(2-)-N29,N30,N31,N32]copper

EC Number:

248-236-5

EC Name:

[29H,31H-phthalocyaninetrisulphonyl trichloridato(2-)-N29,N30,N31,N32]copper

Cas Number:

27121-30-8

Molecular formula:

C32H13Cl3CuN8O6S3

IUPAC Name:

Copper, [29H,31H-phthalocyanine-C,C,C-trisulfonyl trichloridato(2-)-kN29,kN30,kN31,kN32]-

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): waste water treatment plant Frankfurt-Sindlingen, washed, centrifuged and resuspended.
- Pretreatment: 4 days aeration to eliminate the carbon contained in the sludge
- Concentration of sludge: 2.4 mL/L sludge suspension (50 g/L of the centrifuged sediment)
- Dry content: 10.3 g/L corresponding to 25 mg/L dry content in the endvolume of the test batch

sludge was not adapted to test item and reference substance.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

206 mg/L

Based on:

test mat.

Details on study design:

TEST SYSTEM
- Culturing apparatus: 2 L Erlenmeyer flasks
- Number of culture flasks/concentration: 2

- Details of trap for CO2 and volatile organics if used:
- Other:

SAMPLING
- Sampling frequency: after 3h, 1, 4, 7, 11, 14, 18, 21 and 28 days
- Sampling method: 20 mL samples were taken respectively

Shaking machine with about 100 rpm
Temperature 20-22°C

Reference substance

Reference substance:

benzoic acid, sodium salt

Remarks:

concentration: 20 mg/L (calculated as DOC)

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (DOC removal)

Value:

0

Sampling time:

1 d

% Degradation 2

Parameter:

% degradation (DOC removal)

Value:

0

Sampling time:

14 d

% Degradation 3

Parameter:

% degradation (DOC removal)

Value:

0

Sampling time:

28 d

BOD5 / COD results

Results with reference substance:

For the reference substance > 75 % degradation was observed during 14 days proving the validity of the test system.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

No degradation was observed during the 28 d study. Therefore, the test item has to be considered as not readily biodegradable.

Executive summary:

A test for ready biodegradation was performed with the test item according OECD 301 A. Under test conditions no biodegradation was observed after 28 days. The reference substance sodium benzoate reached complete degradation after 7 days.