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Diss Factsheets

Ecotoxicological information

Long-term toxicity to fish

Currently viewing:

Administrative data

Endpoint:
fish early-life stage toxicity
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Remarks:
Basic data given: comparable to guideline but missing analytical data

Data source

Reference
Reference Type:
publication
Title:
Effect of sodium tripolyphosphate (STPP) on the embryonic developmental stages of Zebrafish Brachydanio (danio) rerio
Author:
Sinha P and Kanamadi RD
Year:
2000
Bibliographic source:
Environment and ecology 18(4): 1007-1010

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 212 (Fish, Short-term Toxicity Test on Embryo and Sac-Fry Stages)
Deviations:
yes
Remarks:
No data on dissolved oxygen or test substance concentration over time are given.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Pentasodium triphosphate
EC Number:
231-838-7
EC Name:
Pentasodium triphosphate
Cas Number:
7758-29-4
Molecular formula:
Na5P3O10 H5-xP3O10Nax (where x is approximately 5) 6H2O.Na5P3O10
IUPAC Name:
Pentasodium triphosphate
Details on test material:
- Name of test material (as cited in study report): Sodium tripolyphosphate

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
not specified

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Zebra fish
- Source: Local commercial supplier


METHOD FOR PREPARATION AND COLLECTION OF FERTILIZED EGGS
- Numbers of parental fish (i.e. of females used to provide required number of eggs): 20
- Method of collection of fertilised eggs: Not stated

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
96 h
Post exposure observation period:
None

Test conditions

Hardness:
110 mg/l (as CaCO3)
Test temperature:
26-27°C
pH:
7.4
Dissolved oxygen:
6.9 mg/l
Details on test conditions:
TEST SYSTEM
- No. of organisms per vessel:10


OTHER TEST CONDITIONS
- Photoperiod:14hL/14hD

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LOEC
Effect conc.:
5 mg/L
Basis for effect:
mortality
Details on results:
- Mortality/survival at embryo and larval stages: 4%
- Other biological observations: Abnormal pigmentation in some. Early hatch

Any other information on results incl. tables

Teratogenic effects of different concentrations of sodium tripolyphosphate on Brachydanio embryos

Concentration mg/l

No of embryos exposed

% mortality at 96h

% abnormality at 96h

Abnormality

Control

50

0

0

-

5

50

4

24

Abnormal pigmentation. Some early hatch

10

50

12

48

Many embryos showed abrupt cell proliferations during cleavage. Less pigmentation and absence of it in some cases

20

50

58

100

40

50

86

100

Retarded growth, early hatch, abnormal body structure in some

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
The LOEC of sodium tripolyphosphate was found to be 5 mg/L.