Tetrahydro-2-isobutyl-4-methyl-2H-pyran

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP compliant guideline study with concentration control analytics

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2008

Report date:

2008

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Reaction mass of (2S-cis)-tetrahydro-4-methyl-2-(2-methyl-1-propenyl)-2H-pyran and Tetrahydro-4-methyl-2-(2-methylpropen-1-yl)pyran (2R,4R) and Tetrahydro-4-methyl-2-(2-methylpropen-1-yl)pyran (2S,4S) and (2R-cis)-tetrahydro-4-methyl-2-(2-methyl-1-propenyl)-2H-pyran

EC Number:

939-429-1

Cas Number:

16409-43-1

Molecular formula:

C10H18O

IUPAC Name:

Reaction mass of (2S-cis)-tetrahydro-4-methyl-2-(2-methyl-1-propenyl)-2H-pyran and Tetrahydro-4-methyl-2-(2-methylpropen-1-yl)pyran (2R,4R) and Tetrahydro-4-methyl-2-(2-methylpropen-1-yl)pyran (2S,4S) and (2R-cis)-tetrahydro-4-methyl-2-(2-methyl-1-propenyl)-2H-pyran

Details on test material:

- Chemical name: Tetrahydro-4-methyl-2-(2-methylprop-1-enyl)pyran
- Purity: 99.30 %

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Details on test solutions:

- A saturated solution was prepared with a slow-stirring procedure in a closed bottle.
- 2 x 500 mg of test substance were applied to 2 x 5 L test medium in a brown glass bottle and mixed with a magnetic stirrer for 24 h at 20°C +/- 2
- Appropriate amounts of the saturated solution and dilution water were filled into test vessels; this procedure was carried out daily to provied fresh solution for each day

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

- Origin: Aquarium am AEGI, Volgersweg 6, 30175 Hannover
- Holding: Holding was performed at testing facility at 23 +/- 2°C and diffuse light (0.1 - 10 µmol photons * m-2 * s-1, natural photoperiod)
Water exchange at least once per week
Dissolved oxygen concentration > 80%
- Holding water: tap water of local origin; water was filtered on activated charcoal and aerated for at least 24 h to remove chlorine
Holding water parameters: total water hardness 10 - 250 mg CaCO3/L
pH-value: 6.0 - 8.5
- Average body weight of fish at test initiation: 0.16 g
- Average body length of fish at test initiation: 2.6 cm
- Disease treatment: none
- Zebrafish with at least 12 days of acclimatisation and mortality < 5% before study initiation were used in test
- Feeding: food provided 3 times a week; amount of food was 4% of the fish body weight per feeding day
fish were not fed 24 h prior to test initiation
food: Stör pellets, SERA GmbH, 52518 Heinsberg

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

57.5 - 62.5 mg/L as CaCO3

Test temperature:

20.2 - 23.1°C

pH:

7.26 - 8.06

Dissolved oxygen:

83 - 100 %

Nominal and measured concentrations:

- Concentrations (mg/L): 6.92, 12.5, 25.7, 56.6, 106.5 (measured)

Details on test conditions:

TEST SYSTEM
- Test vessel: glass-aquaria loosely covered with glass lid
- Test volume: 4 L
- Number of test fish: 7 per concentration and control
- Loading: fish density was less than 1 g fish per litre test solution
- Replicates: one replicate per test concentration and control
- Introduction of fish: fish were introduced randomly to individual replicates
- Feeding of fish: none during test

FURTHER TEST CONDITIONS
- Aeration: no aeration was provided with regard of the high volatility of test substance
- Dilution water: same as for holding
- Light intensity: 0.1 - 10 µmol photons * m-2 * s-1, natural photoperiod

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

77.6 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

96 h LC0 56.6 mg/L measured
96 h LC100 106.5 mg/L measured
96 h NOEC 12.5 mg/L measured