Dodecyl(2-hydroxy-3-sulphonatopropyl)dimethylammonium

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

June to August 1996

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Remarks:

One main deviation noted, no chemical analysis was performed. The report is brief and doesn’t include background details of culturing etc.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

1992

Deviations:

yes

Remarks:

One main deviation noted, no chemical analysis was performed.

GLP compliance:

yes

Specific details on test material used for the study:

Betadet S-20
Batch number 2GR043.0001

The Certificate of Analysis (not included in the report) provides the following information:
Batch: 2GR043.0001 (19/10/1995)
Appearance: Liquid, transparent, slightly yellowish fluid
Dry matter: 45%
pH: 6.8
Colour: 36 APHA units
Sodium chloride: 7.1%

The principal constituent (included in the 45% 'dry matter') represented approximately 30% (w/w) of the tested batch.

Analytical monitoring:

no

Details on sampling:

Not applicable

Vehicle:

no

Details on test solutions:

The test material was dissolved in water.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Fish were obtained from a commercial supplier: Meridiana Aquarium SA, Barcelona and were held in quarantine for 12 days prior to test initiation. The fish were fed daily, up to 24h before the start of the test.
Fish were held is glass aquaria with a capacity of 84 L, pH was in the range of 8.0 - 8.6 and temperature was maintained between 20 and 24°C.
Fish were 3.4 - 3.8 cm at the end of the preliminary study and 3.0 - 3.5 cm at the end of the definitive test.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Remarks on exposure duration:

None

Post exposure observation period:

Not applicable

Hardness:

195.8 - 231.4 mg/L as CaC03 during the definitive study

Test temperature:

21.0 - 25.9°C

pH:

8.15 - 8.46 during the definitive study

Dissolved oxygen:

Maintained between 65 and 90% of the Air Saturation Value

Salinity:

Not applicable (freshwater test)

Conductivity:

Not applicable

Nominal and measured concentrations:

Nominal exposure concentrations - 0 (control), 100, 141.4, 168.2, 200 and 400 mg/L.

Details on test conditions:

Test vessels used in the definitive test were glass aquaria containing 50 L of test medium.
20 fish were exposed in the control and 100, 168.2 and 200 mg/L treatments and 10 were exposed at the 141. and 400 mg/L treatments.

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

55.2 mg/L

Nominal / measured:

nominal

Conc. based on:

other: principal constituent

Basis for effect:

mortality (fish)

Remarks on result:

other: Calculated from reported endpoint as (184 x 0.3), based on 30% (w/w) principal constituent content of tested batch.

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

184 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Endpoint determined by graphical interpolation from the mortality response curve.

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

168.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

< 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

behaviour

Remarks on result:

other: Symptoms included inactivity, sounding in the aquaria, cessation of swimming and rapid respiration.

Details on results:

The 96 h LC50 for Betadet S-20, based on nominal exposure concentrations was determined graphically to be 184 mg/L. This is equivalent to 55.2 mg principal constituent/L after correction for the 30% (w/w) p.c. content of the tested batch of Betadet S-20.

Results with reference substance (positive control):

Not applicable

Reported statistics and error estimates:

No statistical analyses were performed. The LC50 was determined graphically.

Sublethal observations / clinical signs:

Mortality observations

Nominal concentration (mg/L)	No. of fish exposed	Cumulative mortality					% mortality at 96 hours
		3 hours	24 hours	48 hours	72 hours	96 hours
Control	20	0	0	0	0	0	0
100	20	0	0	0	0	0	0
141.4	10	0	0	0	0	0	0
168.2	20	0	0	0	0	0	0
200	20	0	14	17	19	19	95
400	10	10	10	10	10	10	100

 

Validity criteria fulfilled:

yes

Conclusions:

The 96 h LC50 for Betadet S-20, based on nominal exposure concentrations was determined graphically to be 184 mg/L. This is equivalent to 55.2 mg principal constituent/L after correction for the 30% (w/w) p.c. content of the tested batch of Betadet S-20.

Executive summary:

The acute toxicity of Betadet S-20 to Zebra fish (Brachydanio rerio) was determined in a static study carried out according to OECD test guideline 201 (1992). Fish were exposed to nominal test concentrations of 0 (control), 100, 141.4, 168.2, 200 and 400 mg/L for 96 hours. No analytical verification was performed therefore the results of the study are based in the nominal exposure concentrations. 

The 96 h LC50 for Betadet S-20 was determined graphically to be 184 mg/L with a corresponding NOEC of 168.2 mg/L, based on mortality. The LC50 and NOEC equate to 55.2 and 50.5 mg principal constituent/L, respectively, after correction for the p.c. content of the tested batch of Betadet S-20.

Description of key information

The 96 h LC50 for Danio rerio exposed to Betadet S-20 was determined to be 184 mg/L with a corresponding NOEC of 168.2 mg/L, based on mortality. The LC50 equates to 55.2 mg principal constituent/L after correction for the p.c. content of the tested batch of Betadet S-20.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

55.2 mg/L