2-chloro-3-oxopentyl acetate

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

21.02-29.03.2001

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: test according to revised OECD-Principles of Good Laboratory Practice, Paris, 1997; Guideline OECD 301E

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2-chloro-3-oxopentyl acetate

EC Number:

235-930-8

EC Name:

2-chloro-3-oxopentyl acetate

Cas Number:

13051-49-5

Molecular formula:

C7H11ClO3

IUPAC Name:

2-chloro-3-oxopentyl acetate

Details on test material:

not relevant

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Melting point: < -30 °C
- Boiling point: 246
- Vapour pressure: 1.3 mbar
- Water solubility (under test conditions): 1.00 mg/l

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, predominantly domestic, adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): secondary effluent of a treatment plant

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

40 other: ppm

Based on:

DOC

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

TEST CONDITIONS
- Composition of medium: solution a: Kaliumdihydrogenphosphate (KH2P04) 83 g, di-Kaliumhydrogenphosphate (K2HPOS 21,75 g
di-Natriumhydrogenphosphate-Dihydrat (Na2HP04*2H20) 33'4 g, Ammoniumchlorid (NH4CI) 0,5 g, Aqua demin. ad l000 ml
solution b: Calciumchloriddihydrate (CaCI2*2H20) 36,4 g, Aqua demin. ad l000 ml
solution c: Magnesiumsulfatheptahydrate (MgS04*7H20) 22,5 g, Aqua demin. ad l000 ml
solution d: Irion(lll)chloridehexahydrate (FeCI3*6H20) 025 g, Dinatriumethylendiamintetraacetate (Na2EDTA) 0,4 g, Aaua demin. ad l000 ml
solution e: Mangansulfattetrahydrate (MnS04*4H20) 39.9 mg/l, boric acid (H3BO3) 57.2 mg/l, Zinksulfatheptahydrate (ZnS04*7H20) 42.8 mg/l, Ammoniumheptamolybdate ((NH4)6(Mo7024)) 34.7 mg/l, Iron-chelate (FeCI3-EDTA) 100 mg/l, Aqua demin. ad 1000 mg/l
solution f: yeast extract 15 mg, add water up to 100 ml
test solution: solution a 10 ml, solution b 1 ml, solution c 1 ml, solution d 1 ml, solution e 1 ml, solution f 1ml, Aqua demin. ad 1000 ml
- Test temperature: 21 + 2 °C
- Continuous darkness: no


TEST SYSTEM
- Measuring equipment: Shimadzu TOC-5050A


SAMPLING
- Sampling frequency: after 2, 5, 9, 14, 20 und 28 days

CONTROL AND BLANK SYSTEM
- Inoculum blank:
- Abiotic sterile control: 1% Hg2Cl2-solution
- Toxicity control: Anillin - solution

Reference substance

Reference substance:

aniline

Results and discussion

Preliminary study:

paren solution: 500 mg/l; dilution 1:5; carbon content determined (DOC measure)

Test performance:

see result

% Degradation

Parameter:

% degradation (DOC removal)

Value:

50

Sampling time:

28 d

Details on results:

Chlorketone is not readily biodegradable.

BOD5 / COD results

Results with reference substance:

Reference substance: Aminobenzene
Degradation of Aminobenzene: 95% after 5 days

Any other information on results incl. tables

no additional remarks

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

other: biodegradable, but criteria for ready biodegradability not met

Conclusions:

The substance was found to be biodegradable. However, the pass level for ready biodegradability was not reached.

Executive summary:

Chlorketone was tested for its ready biodegradability in the modified OECD screening test.

The study procedure was based on OECD guideline No. 301 E, July 17, 1992.

The test piece was added in a quantity of 40 ppm DOC to the test medium.

The inoculum was derived from the secondary effluent of a treatment plant. Anillin was used as reference substance. The observed biodegradability was 89% after 28 days. In the abiotic sterile control the degradability was 40% and therefore the biodegradability of this substance is 50%.

The criterion for ready biodegradability (at least 70% removal of DOC in a 10 -day window) was therefore not met in these experiment.

The substance was found to be biodegradable. However, the pass level for ready biodegradability was not reached.