Mercury sulphate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

calculation (if not (Q)SAR)

Remarks:

Migrated phrase: estimated by calculation

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data is from peer reviewed journal

Data source

Reference

Reference Type:

publication

Title:

Influence of glyphosate and its formulation (Roundup) on the toxicity and bioavailability of metals to Ceriodaphnia dubia

Author:

Martin T.K. Tsui, Wen-Xiong Wang , L.M. Chu ,

Year:

2005

Bibliographic source:

Environmental Pollution 138 (2005) 59-68

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: as per below mentioned

Principles of method if other than guideline:

Short term toxicity was performed on Ceriodaphnia dubia (Neonate) for 48 hrs to calculate LC 100 value.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Mercury sulphate

EC Number:

231-992-5

EC Name:

Mercury sulphate

Cas Number:

7783-35-9

Molecular formula:

H2O4S.Hg

IUPAC Name:

mercury sulfate

Details on test material:

- Name of test material: Mercury sulphate
- Molecular formula: H2O4S.Hg
- Molecular weight : 298.67 g/mol
- Smiles notation: [O-]S(=O)(=O)[O-].[Hg+2]
- InChl : InChI=1S/Hg.H2O4S/c;1-5(2,3)4/h;(H2,1,2,3,4)/q+2;/p-2
- Substance type: Solid
- Physical state: Inorganic
- Impurities (identity and concentrations): No data

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
No data available

Sampling and analysis

Analytical monitoring:

not specified

Details on sampling:

No data available

Test solutions

Vehicle:

not specified

Details on test solutions:

No data available

Test organisms

Test organisms (species):

Ceriodaphnia dubia

Details on test organisms:

Details on test organisms
TEST ORGANISM
- Common name: Water flea
- Strain: No data available
- Source: Aquatic Research Organisms (Hampton, NH, USA)
- Age at study initiation (mean and range, SD): Neonate (<24 hrs)
- Weight at study initiation (mean and range, SD): No data available
- Length at study initiation (length definition, mean, range and SD): No data available
- Valve height at study initiation, for shell deposition study (mean and range, SD): No data available
- Peripheral shell growth removed prior to test initiation: No data available
- Method of breeding: No data available
- Feeding during test: not fed during the test
- Food type: Selenastrum capricornutum and yeastecerophyle
trout chow
- Amount: No data available
- Frequency: No data available

ACCLIMATION
- Acclimation period: No data available
- Acclimation conditions (same as test or not): No data available
- Type and amount of food:
- Feeding frequency: No data available
- Health during acclimation (any mortality observed): No data available

QUARANTINE (wild caught)
- Duration: No data available
- Health/mortality: No data available

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Post exposure observation period:

No data available

Test conditions

Hardness:

No data available

Test temperature:

25°C

pH:

No data available

Dissolved oxygen:

No data available

Salinity:

No data available

Nominal and measured concentrations:

Σ0.5, Σ1.0, Σ1.5, Σ2.0 and Σ2.5 toxic units

Details on test conditions:

Details on test conditions
TEST SYSTEM
- Test vessel: beaker
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 100 ml
- Aeration: No data available
- Type of flow-through (e.g. peristaltic or proportional diluter): No data available
- Renewal rate of test solution (frequency/flow rate): No data available
- No. of organisms per vessel: five organisms were stocked in each replicate (i.e. 10 mL per individual).
- No. of vessels per concentration (replicates): 4 replicates
- No. of vessels per control (replicates): 1 vessels
- No. of vessels per vehicle control (replicates): No data available
- Biomass loading rate: No data available

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: No data available
- Total organic carbon: No data available
- Particulate matter: No data available
- Metals: No data available
- Pesticides: No data available
- Chlorine: No data available
- Alkalinity: No data available
- Ca/mg ratio: No data available
- Conductivity: No data available
- Culture medium different from test medium: No data available
- Intervals of water quality measurement: No data available

OTHER TEST CONDITIONS
- Adjustment of pH: No data available
- Photoperiod: No data available
- Light intensity: No data available

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : No data available

TEST CONCENTRATIONS
- Spacing factor for test concentrations: No data available
- Justification for using less concentrations than requested by guideline: No data available
- Range finding study: No data available
- Test concentrations: Σ0.5, Σ1.0, Σ1.5, Σ2.0 and Σ2.5 toxic units
- Results used to determine the conditions for the definitive study: No data available

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

LC100

Effect conc.:

0.028 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: Σ0.5, Σ1.0, Σ1.5, Σ2.0 and Σ2.5 toxic units

Details on results:

No data available

Results with reference substance (positive control):

No data available

Reported statistics and error estimates:

No data available

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

The LC 100 value for Ceriodaphnia dubia was found to be 20 µg L-1 after 48 hrs of exposure with Sulfuric acid, Mercury(2+) salt(1:1) (7783-35-9)

Executive summary:

Acute toxicity test was performed on Ceriodaphnia dubia for 48 hrs using neonates. The experiment was performed in 100 mL beaker having 50 ml test solution. Experiment design was static with six concentrations having 4 replicates in which five organisms were stocked in each replicates. The tested concentrations in the binary mixtures were

Σ 0.5, Σ 1.0, Σ 1.5, Σ 2.0 and Σ 2.5 toxic units. Mortality was the effect measured in experiment and number of animals was counted at 24 and 48 hrs.

Therefore, on the basis of above experiment the LC 100 value for Ceriodaphnia dubia was found to be 20 µg L^-1after 48 hrs of exposure with Sulfuric acid, Mercury(2+) salt(1:1) (7783-35-9).