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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Description of key information

Acute toxicity: Oral LD50 (rat, m/f): > 2500 mg/kg bw (OECD 423)
Acute toxicity: Dermal LC50 (rat, m): > 5000 mg/kg bw (OECD 402)

Key value for chemical safety assessment

Acute toxicity: via oral route

Endpoint conclusion
Endpoint conclusion:
no adverse effect observed
Dose descriptor:
LD50
Value:
2 500 mg/kg bw

Acute toxicity: via inhalation route

Endpoint conclusion
Endpoint conclusion:
no study available

Acute toxicity: via dermal route

Endpoint conclusion
Endpoint conclusion:
no adverse effect observed
Dose descriptor:
LD50
Value:
5 000 mg/kg bw

Additional information

Oral

The acute oral toxicity of 2-ethylhexyl benzoate was evaluated in rats in accordance with OECD guideline 423 under GLP conditions (Blanchard, 2000).

Sprague-Dawley rats (males and females) were stepwise dosed with the test material (5000 and 2000 mg/kg bw) using three animals of a single sex per step.

At 5000 mg/kg bw 1/3 females and 2/3 males died 48 h post-dose. In the next step at 2000 mg/kg bw only one female animal died (72 h post-dose). All animals showed clinical signs of abnormal gait and hunched posture and further signs were apparent in single animals e.g. lethargy, reduced body temperature being fully reversible within 3 days.

Reduced body weight was observed in one female at 2000 mg/kg bw on Day 8 only.

Necropsy revealed no substance-related findings.

Therefore, the oral LD50 for male and female rats was considered to be 2500 mg/kg bw according to the cut off values of OECD guideline 423.

 

Inhalation

This information is not available.

 

Dermal

The acute dermal toxicity of 2-ethylhexyl benzoate was evaluated according to OECD guideline 403 under GLP conditions (Blanchard, 2000).

A group of five Sprague-Dawley rats (males and females) were treated in a limit test with 5004 mg/kg bw of the test substance.

No mortality occurred during the study period in any animal. No systemic clinical signs of toxicity were observed up to the end of the 14-day observation period. Transient very slight dermal irritation was seen in all animals following removal of the dressing, resolving completely by Day 4.

A loss in body weight was recorded for one female and low body weight gains were noted for the 4 remaining females on Day 8. All other animals were considered to have achieved satisfactory body weight throughout the study.

Necropsy revealed no substance-related findings.

Therefore, the dermal LD50 for male and female rats was considered to be greater than 5000 mg/kg bw.


Justification for selection of acute toxicity – oral endpoint
Only one study available.

Justification for selection of acute toxicity – dermal endpoint
Only one study available.

Justification for classification or non-classification

The available data on acute toxicity of 2-ethylhexyl benzoate do not meet the criteria for classification according to Regulation (EC) 1272/2008, and the data are therefore conclusive but not sufficient for classification.