Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 221-088-9 | CAS number: 3001-98-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 2017-03-20 to 2017-03-24
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 3,9-dimethyl-2,4,8,10-tetraoxa-3,9-diphosphaspiro[5.5]undecane 3,9-dioxide
- EC Number:
- 221-088-9
- EC Name:
- 3,9-dimethyl-2,4,8,10-tetraoxa-3,9-diphosphaspiro[5.5]undecane 3,9-dioxide
- Cas Number:
- 3001-98-7
- Molecular formula:
- C7H14O6P2
- IUPAC Name:
- 3,9-dimethyl-2,4,8,10-tetraoxa-3λ⁵,9λ⁵-diphosphaspiro[5.5]undecane-3,9-dione
- Test material form:
- solid: particulate/powder
- Details on test material:
- AFLAMMIT® PCO 962
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: At the start of the exposure intervals (0 and 72 h), sampling was carried out after preparation of the limit concentration. At the end of the exposure intervals (48 and 96 h), samples were taken directly from the test vessels.
- Sample storage conditions before analysis: All original samples were stored at room temperature until preparation. Prepared samples were stored in an autosampler at room temperature until analysis.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: An appropriate amount of the test item was weighed and transferred into a test vessel filled with an appropriate volume of dilution water. The test media was mixed with an ultraturrax (1 min, 17000 rpm). This procedure was repeated for every water renewal.
- Controls: Dilution water without test item incubated under the same conditions as the limit test item concentration
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): no
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: zebrafish
- Strain: not specified
- Source: All fish used in the test was gained at Noack Laboratorien GmbH from a single brood stock (supplier: Umweltbundesamt, Schichauweg 58, D-12307 Berlin)
- Age at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD): Average body length at the test start: 2.80 cm
- Weight at study initiation (mean and range, SD): Average body weight at the test start: 0.22 g
- Maintenance of the brood fish: Holding was performed at the test facility at 23 ± 2 °C and diffuse light (7 – 750 Lux, natural photoperiod). The water was changed at least once per week. The dissolved oxygen concentration was more than 80 % of the air saturation value.
ACCLIMATION
- Acclimation period: Zebrafish with at least 12 days of acclimatization and mortality < 5 % within the last 7 days before the start of the exposure were used in the test.
- Acclimation conditions (same as test or not): not specified
- Type and amount of food during acclimation: The amount of food was 4 % of the fish body weight per feeding day. Sera vipan food
- Feeding frequency during acclimation: Food was provided 3 times per week.
- Health during acclimation (any mortality observed): No mortality was observed during this time. No disease treatments were administered throughout holding and testing.
FEEDING DURING TEST
Fish were not fed during the test.
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Post exposure observation period:
- not relevant
Test conditions
- Hardness:
- 65 mg/L as CaCO3
- Test temperature:
- 22.9 ± 0.3°C
- pH:
- 7.51-8.02
- Dissolved oxygen:
- oxygen saturation: 85-100%
- Salinity:
- not relevant
- Conductivity:
- 181 µS/cm
- Nominal and measured concentrations:
- 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass aquaria of 13 L volume were used, filled with approx. 10 L of dilution water
- Type (delete if not applicable): Glass aquaria covered with glass lids
- Material, size, headspace, fill volume: 25.5/18/28 cm, depth of water: 20 cm
- Aeration: No aeration was provided.
- Renewal rate of test solution: A semi-static test procedure with daily renewal of the test media was performed.
- No. of organisms per vessel: 7 zebrafish (sexes not specified) were used for each test concentration and control.
- No. of vessels per concentration (replicates): One replicate per test concentration.
- No. of vessels per control (replicates): One replicate per control.
- Biomass loading rate: Fish density in the tanks was 0.16 g fish per litre test solution.
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water of local origin was used for holding and testing. The water was filtered on activated charcoal and aerated for at least 24 hours to remove chlorine
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: light / dark cycle: A natural photoperiod (approx. light / dark cycle: 16 / 8 h) was maintained during exposure.
- Light intensity: 7 – 750 Lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
Observations were made after 3, 24, 48, 72 and 96 h.
Records were kept of visible abnormalities (e.g. loss of equilibrium, swimming behavior, respiratory function, pigmentation, etc.).
TEST CONCENTRATIONS
- Spacing factor for test concentrations: limit test
- Range finding study: no The threshold concentration (TC) is based on a daphnia toxicity test of the test item (nominal concentrations): EC50 > 100 mg/L, SCHEERBAUM, 2018 and an algae toxicity test of the test item (nominal concentrations): ErC50 (0-96 h) > 100 mg/L, EyC50 (0-96 h) > 100 mg/L, SCHEERBAUM, 2018. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: not observed
- Observations on body length and weight: no
- Other biological observations: no
- Mortality of control: no
- Other adverse effects control: no
- Abnormal responses: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no - Results with reference substance (positive control):
- not applicable
- Reported statistics and error estimates:
- The LC0 and LC100 after 96 h were derived from the raw data of the limit concentration. LC50-values do not have to be calculated in a limit test.
The data for the tables in this report were computer generated and have been rounded for presentation from the full derived data. Consequently if calculated manually based on the given data minor variations may occur from these figures.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- All effect levels are given based on the nominal threshold concentration of the test item AFLAMMIT® PCO 962. The threshold concentration of the test item was found to have no effects to zebrafish.
In conclusion, the LC0 corresponds to the nominal concentration of 100 mg test item/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.