Tris(2-hydroxyethyl)-1,3,5-triazinetrione

Long-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: guideline study under GLP

Remarks:

(as given in OECD SIDS)

Qualifier:

according to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

GLP compliance:

yes

Analytical monitoring:

yes

Test organisms (species):

Daphnia magna

Test type:

semi-static

Key result

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

- Analytical monitoring: 92-96 % of the nominal concentration at preparation; 93-97 % just before the renewal of the test water.

- Water chemistry in test (O2, pH): DO (mg/L); 8.2-8.9; pH; 7.2-8.4

- EC50: >100 mg/L (21 d reproduction)

- LC50 for parental Daphnia (21 d): >100 mg/L

- NOEC: >=100 mg/L (21 d reproduction)

- Statistical results, as appropriate: There was no statistically significant difference between data from the control and 100 mg/L test groups.

 

Nominal concentrations (as mg/L): 100 

Measured concentrations (as mg/L): 92-97

 

- Table on measured concentrations:

 

Nominal Concentration	Measured Concentration (mg/L)
(mg/L)	0 day a	2 days b	8 days a	10 days b
Control	<1	<1	<1	<1
100	93	93	92	97

 

Nominal Concentration	Measured Concentration (mg/L)
(mg/L)	20 day a	21 days b	Mean c
Control	<1	<1	<1
100	96	96	94

 

a : freshly prepared test solution

b : old test solution before renewal

c : time-weighted mean

 

- Cumulative numbers of dead parental Daphnia: 

0 % mortality at control and 100 mg/L

- Time of the first brood production of juveniles: 7 d at control and 100 mg/L

 

- Mean cumulative numbers of juveniles produced per live adult: 

____________________________________________________________

 Nominal        Measured      Mean cumulative numbers of 

Concentration  Concentration   juveniles produced 

                                  per live adult  

 (mg/L)         (mg/L)         14 days       21 days

Control            -             59           118

 100            92-97 a         65           118

a : value during the test

- Control response satisfactory: Yes

Conclusions:

There was no statistically significant difference in mean cumulative numbers of juveniles produced per live adult between the treatment group and the control. Hence, 21d-EC50 was greater than 100 mg/L and 21d-NOEC was more than 100 mg/L on chronic Daphnia reproduction test.

Description of key information

NOEC(21 d) ≥100 mg/L for Daphnia magna (OECD TG 211)

Additional information

Value used for CSA: EC10/LC10 or NOEC for freshwater invertebrates: ≥100 mg/L