Bis(4-fluorophenyl) ketone

Bioaccumulation: aquatic / sediment

Administrative data

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2005-11 to 2006-03

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions: analytical method no described in enough detail

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2006

Report date:

2006

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 305 B (Bioaccumulation: Semi-static Fish Test)

Version / remarks:

adopted May 1981

Deviations:

not specified

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

4,4-Difluoro Benzophenone

IUPAC Name:

4,4-Difluoro Benzophenone

Details on test material:

Physical state: white powder
Purity: 99%

Radiolabelling:

no

Sampling and analysis

Details on sampling:

- Sampling intervals/frequency for test organisms: at 30 h, 42 h, 72 h, 96 h, 120 h, 144 h, 240 h, 360 h
- Sampling intervals/frequency for test medium samples: at 0 h, 24 h, 48 h, 72 h, 96 h, 120 h, 144 h, 288 h
- Sample storage conditions before analysis: frozen
- Details on sampling and analysis of test organisms and test media samples (e.g. sample preparation, analytical methods):

Test solutions

Details on preparation of test solutions, spiked fish food or sediment:

A stock solution with a concentration of 100 mg/mL was prepared in acetone

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

- fish were acclimated to laboratory conditons for 2 weeks
- length: 25 - 35 mm, weight: 0.2 - 0.4 g

Study design

Route of exposure:

aqueous

Test type:

semi-static

Water / sediment media type:

not specified

Remarks:

freshwater

Total exposure / uptake duration:

15 d

Total depuration duration:

8 d

Test conditions

Test temperature:

24 +/- 2 °C

pH:

6.95 to 7.04

Dissolved oxygen:

7.9 to 8.1 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- 120 L glass aquaria, filled with 100 L test solution
- Aeration: no:
- Renewal rate of test solution: every 24 hours
- No. of organisms per vessel: 150
- No. of vessels per concentration (replicates): 1

Nominal and measured concentrations:

Nominal: 0.1 mg/L and 0.2 mg/L

Details on estimation of bioconcentration:

- no data

Results and discussion

Bioaccumulation factor

Bioaccumulation factor 1

Type:

BCF

Value:

55.4

Basis:

whole body w.w.

Time of plateau:

240 h

Calculation basis:

steady state

Remarks on result:

other: Conc.in environment / dose:0.1 mg/L

Bioaccumulation factor 2

Type:

BCF

Value:

74.4

Basis:

whole body w.w.

Time of plateau:

240 h

Calculation basis:

steady state

Remarks on result:

other: Conc.in environment / dose:0,2 mg/L

Depuration

Elimination:

yes

Any other information on results incl. tables

Concentration (mg/L)	Cf (mg/kg, mean value)	Cw (mg/L, mean value)	BCF
0.1	5.045	0.091	55.4
0.2	12.581	0.169	74.4

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Executive summary:

The bioaccumulation of 4,4-Difluoro Benzophenone was investigated in a bioaccumulation study with fish according to OECD 305 B. The test species Danio rerio was exposed to the test item at two concentration levels (0.1 and 0.2 mg/L) The uptake phase lasted 15 days (until the steady state was reached) and subsequent elimination was monitored over a period of 8 days. The following steady state BCF values were calculated

Conc. A (0.1 mg/L): BCF = 55.4

Conc. B (0.2 mg/L): BCF = 74.4

The study was assessed as "reliable with restrictions".