Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
18-08-2003 to 15-09-2003
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
Version / remarks:
17 July 1992
Deviations:
no
GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
other: domestic wastewater, secondary effluent
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): secondary effluent from the City of Midland, Michigan Wastewater Treatment Plant, USA, > 90% domestic wastewater
- Storage length: secondary effluent sampled one day prior to study initiation
- Preparation of inoculum for exposure:
- Pretreatment: effluent filtered through Whatman 114V filter paper prior to use
- Concentration of sludge: 4 mL/L
Duration of test (contact time):
28 d
Initial conc.:
2.64 mg/L
Based on:
act. ingr.
Parameter followed for biodegradation estimation:
O2 consumption
Remarks:
Orion model 97-08 dissolved oxygen electrode connected to an Orion model 920A + pH meter.
Details on study design:
TEST CONDITIONS
- Composition of medium: according to OECD 301D (mineral medium)
- Additional substrate: no
- Solubilising agent (type and concentration if used): no
- Test temperature: 19.2±0.3 °C (range: 18.6 to 19.7 °C)
- pH: 7.4
- pH adjusted: no
- Aeration of dilution water: aeration of inocultaed medium for at least 30 min and allowed to stand for at least 20 h prior to adding the test and reference material
- Continuous darkness: yes

TEST SYSTEM
- Culturing apparatus: 300-mL glass BOD bottles, filled to capacity, and sealed with ground glass stoppers and plastic covers to prevent entrapment of air bubbles and evaporation of the aqueous media
- Number of culture flasks/concentration: 18 replicates
- Measuring equipment: Orion model 97-08 dissolved oxygen electrode connected to an Orion model 920A + pH meter.
- Test performed in closed vessels due to significant volatility of test substance: yes
- Test performed in open system: no

SAMPLING
- Sampling frequency: after 0, 3, 5, 7, 10, 14, 17, 21, 28 days


CONTROL AND BLANK SYSTEM
- Inoculum blank: 18 replicates (inoculated mineral medium)
- Abiotic sterile control: 0
- Toxicity control: 6 replicates (inoculated mineral medium + 4 mg/L sodium benzoate + 2.64 mg/L test material)
- Other: Procedure control: 6 replicates (inoculated mineral medium + 2.64 mg/L test material)
Reference substance:
benzoic acid, sodium salt
Remarks:
4.02 mg/L
Parameter:
% degradation (O2 consumption)
Value:
41
Sampling time:
3 d
Parameter:
% degradation (O2 consumption)
Value:
61.4
Sampling time:
10 d
Key result
Parameter:
% degradation (O2 consumption)
Value:
64.1
Sampling time:
28 d
Results with reference substance:
The reference compound, sodium benzoate (4.02 mg/L), achieved > 60% biodegradation (i.e., 80.6%) after 14 days.

Dissolved oxygen

Test Suspensions

Inoculum Blanks

(2.64 mg/L valeraldehyde)

(mineral medium + inoculum only)

Sampling Interval (days)

Dissolved Oxygen (mg/L)

Sampling Interval (days)

Dissolved Oxygen (mg/L)

0

8.74

0

8.74

0

8.75

0

8.72

3

5.93

3

8.72

3

5.88

3

8.72

5

5.12

5

8.63

5

4.92

5

8.64

7

4.77

7

8.68

7

4.62

7

8.71

10

4.32

10

8.59

10

4.41

10

8.58

14

4.25

14

8.44

14

4.29

14

8.58

17

4.34

17

8.38

17

4.12

17

8.50

21

3.97

21

8.41

21

3.86

21

8.35

28

3.38

28

7.78

28

3.38

28

7.78

 

 

Summary of biodegradation

Incubation Time (days)

% Biodegradation (mean ± 1 Standard Deviation; n=2)

Test Chemical 

Positive Controls

Toxicity Controls

0

0

0

0

3

41.0±0.5

-

-

5

52.6±2.1

-

-

7

58.2±1.5

78.5±0.6

62.7±0.1

10

61.4±0.9

-

-

14

61.7±0.4

80.6±0.1

61.4±0.1

17

61.3±2.3

-

-

21

65.0±1.1

-

-

28

64.1±0

-

-

Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
Valeraldehyde is readily biodegradable (>60% after 28 days, according to OECD 301D, 1992), fulfilling the 10-day window.
Executive summary:

The ready biodegradability of valeraldehyde was tested in a Closed Bottle Test according to OECD 301D (version 1992). The secondary effluent of a domestic wastewater treatment plant served as inoculum. The samples were incubated for 28 days at 20 °C. The oxygen consumption was observed over a period of 28 days. After 28 days the test substance was degraded to 64.1 % (oxygen consumption). The 10-days window was met.

Valeraldehyde is readily biodegradable according to the pass levels of OECD test guideline 301 (Ready Biodegradability).

Description of key information

Valeraldehyde is readily biodegradable according to OECD criteria, fulfilling the 10-day window (64.1%, Closed Bottle Test, OECD 301D; Dow, 2004).

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

The ready biodegradability of valeraldehyde was tested in a valid Closed Bottle Test according to OECD 301D (version 1992). The secondary effluent of a domestic wastewater treatment plant served as inoculum. The samples were incubated for 28 days at 20 °C. The oxygen consumption was observed over a period of 28 days. After 28 days, the test substance was degraded to 64.1 % (oxygen consumption). The 10-days window was met (Dow, 2004; KS, RL1).

 

In a Zahn-Wellens test (Hoechst AG, 1979, SS, RL2), 67% biodegradation of valeraldehyde within 5 days was determined.

 

In a study using the BOD test according to Standard Methods for the Examination of Water and Wastewater (Waggy/UCC 1993, RL 3 due to insufficient documentation), valeraldehyde was demonstrated to be readily biodegradable (67% biodegradation within 10 days).

 

Valeraldehyde is readily biodegradable according to the pass levels of OECD test guideline 301 (Ready Biodegradability).