Ethylene dimethacrylate

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

30-10-1989 to 08-11-1989

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Remarks:

equals OECD 209, GLP.

Qualifier:

according to guideline

Guideline:

ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)

Deviations:

no

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

no

Details on sampling:

- Sampling method: samples of activated sludge were withdrawn from a sewage plant mainly fed with domestic sewage
- Activated sludge was aerated during transportation to the laboratory
- Concentration(suspended solids): adjusted to 3g/l
- Temp: 20 ±2 °C
- pH: 6-8 (adjustment was not necessary)

Details on test solutions:

- Controls one blanc control with inoculum but without test material, one physico-chemical control (with test - respectively reference- substance,
but without activated sludge, to determine the chemical oxygen consumption)

Test organisms (species):

activated sludge of a predominantly domestic sewage

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

180 min

Remarks on exposure duration:

Determination of the EC50 after 30 min exposure.

Test temperature:

20 ± 2°C

pH:

6.0 - 8.0

Nominal and measured concentrations:

100, 300, 700, 1000, and 3500 mg/l

Details on test conditions:

Incubation period: 30 or 180 min

Reference substance (positive control):

yes

Remarks:

 3,5-dichlorophenol

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

570 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Remarks on result:

other: based on oxygen consumption, graphical determination

Key result

Duration:

180 min

Dose descriptor:

EC50

Effect conc.:

570 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Remarks on result:

other: based on oxygen consumption, graphical determination

Duration:

30 min

Dose descriptor:

EC10

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Remarks on result:

other: based on oxygen consumption

Duration:

180 min

Dose descriptor:

EC10

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Remarks on result:

other: based on oxygen consumption

Results with reference substance (positive control):

- Results with reference substance valid?: Yes

The oxygen consumption (mg/l x h) was calculated from the linear part of the curves of the oxygen concentration versus time.

The percentage inhibition was calculated at each concentration as follows:

I(%) =[1- (Rs - Rpc)/ Rc)]x100

I = percentage  inhibition of oxygen consumption

Rs = oxygene consumption rate by the test mixture

Rc =  oxygen consumption rate by the blanc control

Rpc= oxygen uptake rate by the physico-chemical control  

Table 2: Oxygen uptake and inhibition (%) of the test substance Ethyleneglycol dimethacrylate after a contact time of 30 min and 180 min

 

	O2 uptake (mg/l*h) 30 min	Inhibition (%) 30 min	O2 uptake (mg/l*h) 180 min	Inhibition (%)  180 min
Control C	45.60		73.71
1 mg/l	45.12	1	67.20	9
10 mg/l	47.40	-4	63.94	13
100 mg/l	45.48	0	67.50	8
1000 mg/l	10.47	77	9.92	87
10000 mg/l	5.93	87	4.61	94
phsico-chemical control	0.00		0.00

	O2 uptake (mg/l*h) 30 min	Inhibition (%) 30 min	O2 uptake (mg/l*h) 180 min	Inhibition (%)  180 min
Control C	41.10		82.32
100 mg/l	38.33	7	75.60	8
350 mg/l	30.80	25	61.20	26
700 mg/l	15.53	62	36.25	56
1000 mg/l	9.13	78	18.13	78
3500 mg/l	5.40	87	8.53	90

Validity criteria fulfilled:

yes

Remarks:

The EC50 of 3,5-Dichlorphenol was in the accepted range of 5 to 30 mg/L.

Conclusions:

In a valid respiration inhibition test according (Pseudomonas putida) to ISO 8192 (equivalent to OECD 209) after a contact time of 180 minutes, the EC50 was determined to be 570 mg/L (graphical determination).

Executive summary:

In a valid respiration inhibition test (Pseudomonas putida) according to ISO 8192 (equivalent to OECD 209) after a contact time of 180 minutes, the EC50 was determined to be 570 mg/L (graphical determination).

NOTE: Any of data in this dataset are disseminated by the European Union on a right-to-know basis and this is not a publication in the same sense as a book or an article in a journal. The right of ownership in any part of this information is reserved by the data owner(s). The use of this information for any other, e.g. commercial purpose is strictly reserved to the data owners and those persons or legal entities having paid the respective access fee for the intended purpose.

Description of key information

The toxicity of the substance to microorganisms (Pseudomonas putida) was evaluated in an activated sludge respiration inhibition test

similar to OECD guideline 209 (Klimisch score 1).

The 3h-EC50 was determined to be 570 mg/l for Ethyleneglycol dimethacrylate.

Key value for chemical safety assessment

EC50 for microorganisms:

570 mg/L

Additional information

The toxicity of the substance to microorganisms (Pseudomonas putida) was evaluated in an activated sludge respiration inhibition test

similar to OECD guideline 209 (Klimisch score 1).

The 3h-EC50 was determined to be 570 mg/l for Ethyleneglycol dimethacrylate.