4-methyl-m-phenylenediamine

Toxicity to microorganisms

Administrative data

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Fairly detailed study based on the microtox procedure.

Data source

Reference

Reference Type:

publication

Title:

Ecotoxicity characterization of dinitrotoluenes and some of their reduced metabolites

Author:

Dodard SG, Renoux AY, Hawari J, Ampleman G, Thiboutot S & Sunahara GI

Year:

1999

Bibliographic source:

Chemosphere 38 (9): 2071-9

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Microtox manual; microtox basic test procedures (Microbics Corp, 1992)

Principles of method if other than guideline:

The toxic effects of 2,4-TDA were investigated using the 15-minute Microtox (Vibrio Fischeri) test.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

4-methyl-m-phenylenediamine

EC Number:

202-453-1

EC Name:

4-methyl-m-phenylenediamine

Cas Number:

95-80-7

Molecular formula:

C7H10N2

IUPAC Name:

4-methylbenzene-1,3-diamine

Details on test material:

2,4-TDA
2,4-diaminotoluene
Obtained from Anachemia (St Pierre, Quebec).
The test substance was of the highest grade of purity available.

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

yes

Details on test solutions:

Stock solutions were prepared in DMSO and then dissolved in water to a final concentration of 0.25% DMSO. (This concentration of DMSO was not toxic to the test organisms)

Test organisms

Test organisms (species):

Vibrio fisheri

Details on inoculum:

Microorganisms obtained from Azur Environmental Inc (Carlsbad, California)

Study design

Test type:

static

Water media type:

saltwater

Limit test:

no

Total exposure duration:

15 min

Test conditions

Hardness:

No data.

Test temperature:

15 +/- 1°C

pH:

6.0 - 7.5

Dissolved oxygen:

No data.

Salinity:

2%

Nominal and measured concentrations:

Approximately 12 different concentrations used.

Details on test conditions:

A Microtox Analyzer Model 500 (Azur Environmental Inc, Carlsbad, CA) used for the bioassays.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

15 min

Dose descriptor:

other: IC50

Effect conc.:

405.7 µmol/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Effect concentrations 2

Duration:

15 min

Dose descriptor:

other: IC20

Effect conc.:

102.3 µmol/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Any other information on results incl. tables

IC50 may also be expressed as 49.5mg/l

IC20 may also be expressed as 12.5mg/l