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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Environmental fate & pathways

Biodegradation in soil

Currently viewing:

Administrative data

Endpoint:
biodegradation in soil, other
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Paper presents description of test method and results of degradation step by nitrification. However, not adequate for the purpose of hazard/risk assessment or hazard classification.

Data source

Reference
Reference Type:
publication
Title:
Biodegradation of aromatic ethers by nitrifying bacteria
Author:
Chang, S.W.
Year:
1999
Bibliographic source:
International in situ and on-site bioremediation symposium, 5th, San Diego (7), pp. 131-135

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Cells from a culture of the soil nitrifying bacterium Nitrosomonas europaea were tested for their capability to degrade the test substance in a batch experiment. Die-away of parent and formation of oxidation products in time was detected by HPLC
GLP compliance:
not specified
Test type:
laboratory

Test material

Constituent 1
Chemical structure
Reference substance name:
Veratrole
EC Number:
202-045-3
EC Name:
Veratrole
Cas Number:
91-16-7
Molecular formula:
C8H10O2
IUPAC Name:
1,2-dimethoxybenzene
Test material form:
other: liquid
Details on test material:
no data
Radiolabelling:
no

Study design

Oxygen conditions:
aerobic
Soil classification:
other: not tested in soil; Cell culture of one species
Details on soil characteristics:
not applicable
Parameter followed for biodegradation estimation:
other: analysis of test material and degradation products
Details on experimental conditions:
Nitrosomonas europaea culture in phosphate buffer, 30 degree C

Results and discussion

Transformation products:
yes
Identity of transformation productsopen allclose all
No.:
#1
Reference
Reference substance name:
Unnamed
IUPAC name:
2-methoxyphenol
Inventory number:
InventoryMultipleMappingImpl [inventoryEntryValue=EC 201-964-7]
CAS number:
90-05-1
Identity:
Phenol, 2-methoxy-
Molecular formula:
C7H8O2
Molecular weight:
124.137
SMILES notation:
COc1ccccc1O
InChl:
InChI=1/C7H8O2/c1-9-7-5-3-2-4-6(7)8/h2-5,8H,1H3
No.:
#2
Reference
Reference substance name:
Unnamed
IUPAC name:
catechol
Identifier:
common name
Identity:
catechol
Details on transformation products:
no further details
Evaporation of parent compound:
not specified
Volatile metabolites:
not specified
Residues:
not specified
Details on results:
In general, degradation rates decrease when the number of methoxyl groups on a benzene molecule increase. Therefore veratrole degrades slower than anisole.
Results with reference substance:
no data

Any other information on results incl. tables

Reaction is catalyzed by the non-specific ammonia-oxidizing enzyme ammonia monooxygenase.

Applicant's summary and conclusion

Executive summary:

The test substance is degraded by Nitrosomonas europaea. Transformation products are 2 -methoxyphenol and catechol. The process is catalysed by the presence of the non-specific enzyme ammonia mono-oxygenase (AMO).

The test is performed with a culture of one specific bacterium and does not represent a system or data that can be used to derive degradation rates relevant for the environment. Thus the study is not adequate for hazard/risk assessment or classification.