Registration Dossier
Registration Dossier
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EC number: 200-261-2 | CAS number: 56-18-8
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
Depending on local conditions and existing concentrations, disturbances in the biodegradation process of activated sludge are possible.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 410 mg/L
- EC10 or NOEC for microorganisms:
- 4 mg/L
Additional information
The assessment of the toxicity of 3,3'-iminodi(propylamine) (CAS 56 -18 -8) to aquatic microorganisms is based on experimental data from a respriation inhibition test according to OECD 209, performed under GLP conditions (BASF SE 2011, report no. 08G0696/05G004). Domestic activated sludge was used as inoculum. The 3h-EC50 was determined to be 410 mg/L, the 3h-EC20 was 20 mg/L and the 3h-EC10 was 4 mg/L, based on nominal concentrations.
The results of two additional studies with sludge microorganisms are available:
- In a growth inhibition study with Pseudonomas putida performed according to the German industrial test guideline DIN 38412, Part 8, an 17h-EC50 of 33 mg/L was measured (BASF, 1989, report no. 9/89/9/0499).
- In a short term respiration study with industrial activated sludge performed similar to OECD 209, an EC50 of > 750 mg/L was observed (BASF, 1979, report no. 559).
In all three tests, the concentrations were not analytically verified, but 3,3'-iminodi(propylamine) is expected to be stable in water, as the substance is miscible in water (Gestis database 2012), its vapor pressure is moderate (VP = 1.26 Pa @20°C, BASF AG 1987, report no. 187.0002.1) and its Henry's Law Constant is low (HLC = 5.54 E-08 Pa m³/mol @ 25°C, calculated with HENRYWIN v3.20, not in applicability domain, BASF SE 2017).
In conclusion, depending on local conditions and existing concentrations, disturbances in the biodegradation process of activated sludge are possible.
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