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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: The study has been performed according to OECD and EC guidelines and according to GLP principles.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2007
Report date:
2007

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: OECD guidelines for Testing of Chemicals, guideline No. 203: "Fish Acute Toxicity Test", Adopted 17 July, 1992.
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
-
EC Number:
481-490-7
EC Name:
-
Molecular formula:
multi-constituent.
IUPAC Name:
5,11,17,23,29,35,41,47-octa-tert-butyl-49,50,51,52,53,54,55,56-octahydroxy-2λ⁶,8λ⁶,14λ⁶,20λ⁶,26λ⁶,32λ⁶,38λ⁶,44λ⁶-octathianonacyclo[43.3.1.1³,⁷.1⁹,¹³.1¹⁵,¹⁹.1²¹,²⁵.1²⁷,³¹.1³³,³⁷.1³⁹,⁴³]hexapentaconta-1(49),3,5,7(56),9,11,13(55),15,17,19(54),21(53),22,24,27,29,31(52),33,35,37(51),39(50),40,42,45,47-tetracosaene-2,2,8,8,14,14,20,20,26,26,32,32,38,38,44,44-hexadecone; 5,11,17,23-tetra-tert-butyl-25,26,27,28-tetrahydroxy-2λ⁶,8λ⁶,14λ⁶,20λ⁶-tetrathiapentacyclo[19.3.1.1³,⁷.1⁹,¹³.1¹⁵,¹⁹]octacosa-1(25),3,5,7(28),9,11,13(27),15(26),16,18,21,23-dodecaen-2,2,8,8,14,14,20,20-octone
Details on test material:
- Name of test material (as cited in study report): TC4ASO2
- Substance type: multi-constituent
- Physical state: white powder
- Analytical purity: 97.8%
- Composition of test material, percentage of components: 92.9% 4-tert-Butylsulfonylcalix(4)arene (CAS 204190-49-8)
4.9% 4-tert-Butylsulfonylcalix(8)arene
- Lot/batch No.: AYUI-1Y
- Expiration date of the lot/batch: 07 October 2007
- Stability under storage conditions: Stable
- Storage condition of test material: At room temperature in the dark

Test solutions

Details on test solutions:
Identity and concentration of auxiliary solvent for dispersal: Test concentrations were prepared by stirring a dispersion
of the test substance in test medium (100 mg/l) for 72 hours
and subsequently filtering this mixture through a 0.45 mm
filter to obtain the soluble fraction. An additional test
solution was prepared containing 10% of this filtrate. The
final test solutions were all clear and colourless.

Test organisms

Test organisms (species):
other: Carp (Cyprinus carpio, Teleostei, Cyprinidae)

Study design

Test type:
static

Test conditions

Hardness:
180 mg CaCO3/L

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 0.08 mg/L
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
0.08 mg/L
Details on results:
%Concentration loss over test: ... 69

Any other information on results incl. tables

Sublethal observations / clinical signs:

Comments:
A limit test was performed exposing fish to the WSF of the
test substance. Analysis of the samples taken at the start
(t=0) of the limit test showed a measured concentration of
0.254 mg/l. After 24 hours the test substance concentration
had decreased by approximately 50% and after 96 hours of
exposure, it had further decreased to a measured value of
0.0783 mg/l. This decrease coincided with a precipitation
observed in the test solution during the test period. Hence,
the maximum test substance concentration soluble in the
test medium apparently approximated 0.08 mg/l.


Under the conditions of the test, the test substance induced
no visible effects in carp exposed to a WSF prepared at 100
mg/l and corresponding with an actual concentration of 0.08
mg/l (NOEC). The 96h-LC50 was > 0.08 mg/l, the maximum
concentration soluble in test medium pre-exposed to 100 mg/l
for 72 hours.

Applicant's summary and conclusion