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EC number: 806-509-1
CAS number: 1393571-42-0
Mean OD540 ± SD
Relative mean viability ± SD
0.883 ± 0.087
100 ± 9.8%
0.090 ± 0.051
10.2 ± 5.8%
0.053 ± 0.018
6.0 ± 2.1%
In this assay triplicate reconstructed human skin tissues were treated
with 30 uL of the test substance during 15 minutes. After treatment
tissues were rinsed, kept for 42 hours and treated with MTT. Optical
density of extracted MTT solutions was measured at 540 nm. Viability was
expressed as percentage MTT conversion versus negative control. The
viability was 10.2%, which is indicative for irritant substances.
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