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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Feb 19 - May 25, 2007
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
other: EEC Directive 92/69 and OECD No. 202
Deviations:
no
Principles of method if other than guideline:
none
GLP compliance:
yes (incl. certificate)
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
Reconstituted water according to ELENDT (1990):Macro nutrients (mg/L):CaCI2 x 7 H20 293.80MgSO4 x 7 H20123.30NaHCO364.80KCI5.80Na2SiO3 x 9H2010.00NaNO30.27KH2PO40.14K2HPO40.18Trace elements (mg/L):B0.5000Fe0.2000Mn0.1000Li, Rb and Sr0.0500Mo0.0250Br0.0125Cu and Zn0.0063Co and I0.0025Se0.0010V0.0003Macro nutrients (mg/L):Na2EDTA x 2H20 2.50Vitamins (µg/L):Thiamine75.00B121.00Biotin0.75After preparation, the reconstituded water was aerated for 24 hours. - Hardness: about 14° dH (about 250 mg/L CaCO3)- pH: 7.9 ± 0.3, after an aeration for 24 hours.References:ELENDT, B.-P. Selenium deficiency in Crustacea. An ultrastructural approach to antennal damage in Daphnia magna Straus.Protoplasma 154, 25-33, 1990
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM- Common name: Daphnia magna (Strauss)- Strain: Strauss- Source: in house- Age at study initiation (mean and range, SD): not older than 24 hours- Feeding during test: yes- Food type: suspension of fresh water algae - Amount: sufficient- Frequency: once a week
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Hardness:
14 ° dH (about 250 mg/L CaCO3)
Test temperature:
21-22 °C
pH:
7.2- 7.8
Dissolved oxygen:
90 %
Nominal and measured concentrations:
100 mg/L
Details on test conditions:
-- PreparationThe test medium (reconstituted water and test material) was freshly prepared. Therefore, the test material was solved in the vehicle, reconstituted water-- AdministrationAt the start of the experimental phase, 5 Daphnia magna were placed into 10 ml of reconstituted water (4 vessels/control group) or test medium (4 vessels/ test material group). The Daphnia magna were not fed, and the control medium and test medium were not aerated during the test.The test vessels were labeled to assure unique identification.-- Dose levelsA single test material concentration of 0.1 g/L was tested in an open static system. -- Observation scheduleThe mobility was determined by visual control and recorded after 24 and 48 hours.-- pH and oxygen concentrationThe pH-values and dissolved oxygen concentration (O2) were measured in the control and all test material concentrations at the beginning and at the end of the experimental part.-- TemperatureDuring the experimental part, the temperature was registered in a control vessel with an electronic thermometer containing a maximum and minimum memory display.-- Archive statementAll raw data, specimens and the final report are stored in the archives of the test facility.
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
NOEC
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
A nominal test concentration of 100 mg/L revealed no aquatic toxicity in this test system.
Validity criteria fulfilled:
yes
Conclusions:
Under the given experimental conditions, the test material showed no aquatic toxicity. The 48 hours NOEC to Daphnia magna is > 100 mg/L.
Executive summary:

Purpose

The purpose of this assay was to identify the aquatic toxicity potential of the test material in Daphnia magna to provide a rational basis for hazard estimation for the test item in aquatic environments.

Study design

For this purpose, juvenile Daphnia magna were exposed to an aqueous test material preparation over 48 hours, under defined conditions. The GLP study was performed as a limit test according to OECD TG 202.

Results

The follwing results have been obtained:

   

Nominal concentration Immobilization immobilized / exposed

[g/L]  24 hours  48 hours 
0.0  0/20 0/20 
0.1  0/20  0/20 

Daphnia magna exposed to an aqueous preparation of the nominal concentration of 100 mg per L of test item were not affected.

For the test material the following EC50 values for Daphnia magna were determined:

24 h EC50 > 0,1 g/L (nominal > 100 mg/L)

48 h EC50 > 0,1 g/L (nominal > 100 mg/L)

NOEC > 0.1 g/L (nominal)

LOEC > 0.1 g/L (nominal)

Conclusions

Under the given experimental conditions, the test material showed no aquatic toxicity. The 48 hours NOEC to Daphnia magna is > 100 mg/L.

Description of key information

For this endpoint, adequate information is provided from a GLP compliant OECD TG 202 study, giving a NOEC of > 100 mg/L (nominal).

Key value for chemical safety assessment

Additional information

NOEC > 100 mg/L (nominal)