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Environmental fate & pathways

Bioaccumulation: aquatic / sediment

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Reference
Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
from 2005-06-10 to 2006-08-03
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP study performed according to OECD 305 and METI Guideline: Concentration Test on Chemical Substances in Fish
Qualifier:
according to
Guideline:
other: METI Guideline: Concentration Test on Chemical Substances in Fish
Deviations:
no
Qualifier:
according to
Guideline:
OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Radiolabelling:
no
Details on sampling:
- Sampling intervals/frequency for test organisms: days 0, 7, 14, 21, 25, 28- Sampling intervals/frequency for test medium samples: days 0, 7, 14, 21, 25, 28- Details on sampling and analysis of test organisms and test media samples (e.g. sample preparation, analytical methods):Water: Extraction with CH2Cl2 with further HPLC analysis.Fish: two fish per sampling homogenised, and extracted with acetonitril/n-hexane. The analysis was performed using HPLC.
Vehicle:
yes
Details on preparation of test solutions, spiked fish food or sediment:
According to guideline PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)- Chemical name of vehicle (organic solvent, emulsifier or dispersant): SPAN 20 (Sorbitan Monolaureate) - Concentration of vehicle in test medium (stock solution and final test solution(s) at different concentrations and in control(s)): 0.3 and 0.03 mg/lDose 1: Test item: 0.03 mg/L , Vehicle: 1 mg/LDose 2: Test item: 0.003 mg/L , Vehicle: 2 mg/L
Test organisms (species):
Cyprinus carpio
Details on test organisms:
Weight: 2.46 +/- 0.30 gLength: 6.26 +/- 0.27 cmLot: 50111
Route of exposure:
aqueous
Test type:
flow-through
Water / sediment media type:
natural water: freshwater
Total exposure / uptake duration:
28 d
Total depuration duration:
0
Hardness:
250 mg/L
Test temperature:
25.0 ° +/- 0.4 °C
pH:
7.0
Dissolved oxygen:
7.0 mg/L
Salinity:
According to guideline
Details on test conditions:
After acclimatisation period, the fish (Cyprinus carpio) were transferred into the test media under flow through conditions. Exposure concentrations were analytically verified at days 0, 7, 14, 21, 25 and 28. The analytical values show that the nominal concentrations of 0.03 and 0.003 mg/L were maintained at about 100% using SPON 20, respectively. At days 0, 7, 14, 21, 25 and 28 two fish each were removed from each test group. Body weight was determined. The fish were homogenised and extracted with acetonitrile and n-hexane. The extract was analysed with HPLC.Due to the low bioaccumulation potential, no depuration phase was required.
Nominal and measured concentrations:
nominal concentration 0.03 mg/L: analytically measured concentrations: day 0: 0.0285 mg/L; day 7: 0.0318 mg/L; day 14: 0.0301 mg/Lday 21: 0.0286 mg/Lday 25: 0.0287 mg/Lday 28: 0.0326 mg/Lnominal concentration 0.003 mg/Lanalytically measured concentrations:day 0: 0.00334 mg/Lday 7: 0.00256 mg/Lday 14: 0.00259 mg/Lday 21: 0.00319 mg/Lday 26: 0.00261 mg/Lday 28: 0.00271 mg/L
Details on estimation of bioconcentration:
according to guideline
Type:
BCF
Value:
725 dimensionless
Basis:
normalised lipid fraction
Time of plateau:
14 d
Calculation basis:
steady state
Remarks on result:
other: analytical concentration
Remarks:
Conc.in environment / dose:0.003 mg/L
Type:
BCF
Value:
553 dimensionless
Basis:
normalised lipid fraction
Time of plateau:
21 d
Calculation basis:
steady state
Remarks on result:
other: analytical concentration
Remarks:
Conc.in environment / dose:0.03 mg/L
Details on kinetic parameters:
steady state was reached within 28 daysDue to the low uptake, no depuration phase was necessary
Metabolites:
none
Details on results:
- Mortality of test organisms: no effects- Behavioural abnormalities:no effects- Observations on body length and weight:no effects- Other biological observations: no effects- Bound residues forming a plateau: no- Mortality and/or behavioural abnormalities of control: no- Loss of test substance during test period: no- Results with vehicle control: no effects
Validity criteria fulfilled:
yes
Conclusions:
The test material showed no relevant bioaccumulation at concentrations at or above the water solubility. The steady state BCF values were at 553 and 725 (mean 639). The test material showed no relevant bioaccumulation (BCF < 2000).
Executive summary:

Purpose

The purpose of this study was to determine the bioconcentration potential of the test material in fish under flow-through conditions.

Study Design

The study was performed according to the OECD Guideline for Testing of Chemicals 305 (Bioconcentration: Flow-through Fish Test) adopted on June 14, 1996 using two test material concentrations for exposure to Cyprinus carpio. Two environmental concentrations of the test material of 0.03 and 0.003 mg/L were used in this study. SPAN 20 was used as vehicle at concentrations of 1 and 2 mg/L. The exposure was maintained during the couse of the study at about 100% of the nominal concentrations. The concentrations have been verified analytically using a GC method with FID detection.

Results

No signs of toxicity were observed throughout the study. The analysis reveals steady state BCFs of 553 and 725. Due to the low bioaccumulation potential, no depuration phase was required.

Conclusion

The test material showed no relevant bioaccumulation at concentrations at or above the water solubility. The steady state BCF values were at 553 and 725 (mean 639).

Description of key information

Bioaccumulation was tested in fish (Cyprinus carpio) exposed for 28 days to the test material. Two environmental concentrations of the test material of 0.03 and 0.003 mg/L were used in this study. SPAN 20 was used as vehicle at concentrations of 1 and 2 mg/L. Regular concentration control analysis of the test media showed that the test material concentrations were maintained at 100% of the nominal values, throughout the exposure period and that the test material was present in dissolved form, thus proving steady state exposure conditions. No signs of toxicity were observed throughout the study. Steady state BCFs of 553 and 725 were determined. Due to the low bioaccumulation potential, no depuration phase was required. The steady state BCF values were at 553 and 725 (mean 639). The test item showed no relevant bioaccumulation (BCF < 2000).

Key value for chemical safety assessment

BCF (aquatic species):
639 dimensionless

Additional information