2-(2-(2-butoxyethoxy)ethoxy)ethanol

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

1989

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Scientifically reliable study.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Deviations:

not specified

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

2-(2-(2-methoxyethoxy)ethoxy)ethanol

EC Number:

203-962-1

EC Name:

2-(2-(2-methoxyethoxy)ethoxy)ethanol

Cas Number:

112-35-6

IUPAC Name:

2-[2-(2-methoxyethoxy)ethoxy]ethanol

Constituent 2

Reference substance name:

triethylene glycol monomethyl ether

IUPAC Name:

triethylene glycol monomethyl ether

Details on test material:

>98% triethylene glycol monomethyl ether

Test animals / tissue source

Species:

rabbit

Strain:

New Zealand White

Details on test animals or tissues and environmental conditions:

TEST ANIMALS
- Age at study initiation: 3 - 5 months
- Weight at study initiation: 2.8-3.l kg
- Housing: in a fully air-conditioned rooms in Einzelkafigen (Battery cages)
- Diet: Altromin 2123 Haltungsdiit - rabbits Altromin GmbH, Lage / Lippe, ad libitum and hay (about two cups of coffee 15g)
- Water: deionized, chlorinated water from automatic watering entities, ad libitum

ENVIRONMENTAL CONDITIONS
- Temperature: 20±3 °C
- Humidity: 50 ± 20 %
- Photoperiod: 12 hours a day

Test system

Vehicle:

unchanged (no vehicle)

Controls:

other: The untreated eye was used in each case as a control.

Amount / concentration applied:

0.1 ml of triethylene was applied once into the conjunctival sac of the left eye.

Duration of treatment / exposure:

24 hours

Observation period (in vivo):

No data

Number of animals or in vitro replicates:

3

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

cornea opacity score

Basis:

mean

Time point:

other: 24, 48, 72 hours

Score:

0

Max. score:

4

Results 2

Irritation parameter:

iris score

Basis:

mean

Time point:

other: 24, 48, 72 hours

Score:

0

Max. score:

2

Results 3

Irritation parameter:

conjunctivae score

Basis:

mean

Time point:

other: 24, 48, 72 hours

Score:

0.56

Max. score:

3

Reversibility:

fully reversible within: 72 hours

Results 4

Irritation parameter:

chemosis score

Basis:

mean

Time point:

other: 24, 48, 72 hours

Score:

0

Max. score:

4

Any other information on results incl. tables

INDIVIDUAL FINDINGS:

Time post-application	1h	24h	48h	72h
Animal number	1 2 3	1 2 3	1 2 3	1 2 3
conjunctival swelling	1 1 1	0 0 0	0 0 0	0 0 0
conjunctival redness	1 2 1	1 1 2	0 0 1	0 0 0
Iris	0 0 0	0 0 0	0 0 0	0 0 0
Corneal opacity	0 0 0	0 0 0	0 0 0	0 0 0
Fluorescein-Test		0 0 0		0 0 0

 

Applicant's summary and conclusion

Interpretation of results:

not irritating

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

Slight conjunctival redness was seen with an average score of 0.55 across the three observation timepoints of 24, 48, and 72 hours, but these effects fully resolved across all three animals by the time of the 72 hour observation. On this basis, the substance is regarded as not irritating for classification purposes.

Executive summary:

In a guideline eye irritation study using the substance 2 -(2 -(2 -methoxyethoxy)ethoxy)ethanol, no corneal, iris or chemosis response was seen. Slight conjunctival redness was seen with an average score of 0.55 across the three observation timepoints of 24, 48, and 72 hours, but these effects fully resolved across all three animals by the time of the 72 hour observation. On this basis, the substance is regarded as not irritating for classification purposes.