Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 223-362-3 | CAS number: 3855-32-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 27.08.1996 - 29.08.1996
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- Nov 1989
- Deviations:
- yes
- Remarks:
- See remarks under 'Principles of method if other than guideline'
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- See remarks under 'Principles of method if other than guideline'
- Principles of method if other than guideline:
- Deviation:
pH values were measured in replicate 1 at all concerntrations at 0h and at 48h.
Reported pH values are in the range 7 - 9.1.
According to EU Method C.2 quality criteria the pH should not vary by more than one unit.
According to OECD TG 201 (Daphnia acute mobilisation test) the pH of the test medium should be in the range of 6 to 9.
In the reported results, two samples are reported to be outside this range. (100mg/L at 0h = 9.5 and 50mg/L at 0h = 9.1). In both cases the pH had normalised after 48h to 8.6 and 8.2 respectively.
The affected concentrations, 100mg/L and 50 mg/L were also tested as neutralisd samples (ph range 7.1-7.8).
The testing of the neutralised samples showed a less inhibitory effect on the swimming ability of Daphnia magna.
At 48hrs tested pH values were in the range 7-8.6 for all samples.
The pH of controls (test medium) was 8 at 0h and 48h.
All other recorded pH values are within the range 7-8.6.
The results of the pH determinations are presented in an attachment below.
Although the measured pH ranges vary by more than one unit, the ranges fall within the OECD TG 201 recommendation of a pH rane of 6-9, with the exception of 2 outliers.
The two affected concentrations (100mg/L at 0h = 9.5 and 50mg/L at 0h = 9.1) were also tested as neutralised samples (pH range 7.1-7.8). There is an evident concentration/effect relationship (attached).
Therefore, although the reported pH ranges represent a deviation, the outcomes of the test can be seen as representative. Other validity criteria are satisfied. - GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Substance name: N,N",N"",N""- Pentamethyldipropylentriamin
CAS no.3855-32-1
Purity: 98,5 % (Analytical Report ZAX No. 96L00390)
Stability: stable, storage at room tempe~rature under nitrogen atmosphere - Analytical monitoring:
- yes
- Details on sampling:
- At the start of the test (after 0 hours) samples from vessels which were not stocked with daphnids were analyzed and at the end of the test (after 48 h) samples from stocked and unstocked vessels were analyzed to determine the concentration of the test substance.
The following nominal concentrations of the test substanc~ : were analyzed :
100 mg/L
25 mglL
3,13 mg/ L
0 mg/L (control ) - Vehicle:
- not specified
- Details on test solutions:
- The synthetic medium M4 was used for the culture and test . The medium is prepared on the basis of an ultrapure, deionized water (conductivity < 0 .05 NS/cm).
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- The clone of Daphnia magna STRAUS was supplied by Institut National de Recerche Chimique Appliquee, France, in 1978. The daphnids are cultured under standard conditions in the laboratory.
Animals were aged 2 - 24 hours at the start of the test.
The age of the stock animals was 2-4 weeks. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- None
- Hardness:
- 2.48 mmol/L in test medium.
- Test temperature:
- at T= 0 h: 22.1 degrees C.
at T = 24 h: 21.1 degrees C.
at T= 48 h: 20.8 degrees C. - pH:
- pH values were measured in replicate 1 at all concerntrations at 0h and at 48h.
Reported pH values are in the range 7 - 9.1.
According to EU Method C.2 quality criteria the pH should not vary by more than one unit.
According to OECD TG 201 (Daphnia acute mobilisation test) the pH of the test medium should be in the range of 6 to 9.
In the reported results, only two samples are reported to be outside this range. (100mg/L at 0h = 9.5 and 50mg/L at 0h = 9.1). In both cases the pH had normalised after 48h to 8.6 and 8.2 respectively.
The affected concentrations, 100mg/L and 50 mg/L were also tested as neutralisd samples (ph range 7.1-7.8).
The testing of the neutralised samples showed a less inhibitory effect on the swimming ability of Daphnia magna.
At 48hr tested pH values were in the range 7-8.6 for all samples.
The pH of controls (test medium) was 8 at 0h and 48h.
All other recorded pH values are within the range 7-8.6.
The results of the pH determinations are presented in the attachment below.
Although the measured pH ranges vary by more than one unit, the ranges fall within the OECD TG 201 recommendation of a pH rane of 6-9, with the exception of 2 outliers.
The two affected concentrations (100mg/L at 0h = 9.5 and 50mg/L at 0h = 9.1) were also tested as neutralised samples (pH range 7.1-7.8). There is an evident concentration/effect relationship (attached).
Therefore, although the reported pH ranges represent a deviation, the outcomes of the test can be seen as representative. Other validity criteria are satisfied. - Dissolved oxygen:
- 7.7 mg/L to 8.4 mg/L during duration of test.
- Salinity:
- NA
- Conductivity:
- 0 - 650 NS/cm
- Nominal and measured concentrations:
- At T= 48h, analytical concentration ranged from 97.8% of nominal to 115.0% of nominal concentration. The analytical recovery rate was above 80% in all analysed test solutions after 48 h.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: test tubes (glass) with flat bottom, nominal volume 2
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates):4
- No. of vessels per control (replicates):
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ultrapure deionised water (< 0.05 uS/cm).
- Alkalinity:0.80 - 1.00 mmol/L
- Ca/mg ratio:about 4:1
- Conductivity: 550-650 uS/cm
- Intervals of water quality measurement:0 h; 48 h.
OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod:
- Light intensity:
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations:
- Results used to determine the conditions for the definitive study: - Reference substance (positive control):
- not specified
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 35.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 31.0 mg/L - 40.4 mg/L
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- Above a concentration of about 50 mg/L (nominal), the pH-values of the test samples rise over pH 8.5 at the beginning of the test. In these samples a strong inhibition of the swimming ability of Daphnia magna occured. The testing of the neutralised samples showed a less inhibitory effect on the swimming ability of Daphnia magna.
- Results with reference substance (positive control):
- NA
- Reported statistics and error estimates:
- For the statistical evaluation of the EC50 (48h) the moving average method was used .
- Validity criteria fulfilled:
- yes
- Remarks:
- In the control, immobilisation was less than or equal to 10%. Dissolved oxygen concentration at the end of the test was >3 mg/l in control and test vessels. pH values were outside guideline ranges, however the test is deemed valid, as discussed above.
- Conclusions:
- The acute toxicity of N,N,N',N'',N''-Pentylmethyldipropylentriamin to the water flea Daphnia magna was determined in accordance with guidelines (EU C2 methods for acute toxicity to Daphnia). An EC50 of 35.4 mg/L was determined.
Reference
Description of key information
An acute daphnid toxicity study on the substance was conducted in accordance with EU C2 method, referencing OECD TG 202 (Daphnia sp Acute Immobilsisation Test)
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 35.4 mg/L
Additional information
The acute toxicity of the substance to the water flea Daphnia magna was determined in accordance with guidelines (EU C2 methods for acute toxicity to Daphnia). The following values were determined from the test: 48-h EC0 = 25 mg/L; 48-h EC50 of 35.4 mg/L; and 48-h EC100 = 100 mg/L. Above a concentration of about 50 mg/L (nominal), the pH-values of the test samples rose to a level pH 8.5 at the beginning of the test. In these samples a strong inhibition of the swimming ability of Daphnia magna occurred. The testing of the neutralised samples showed a less inhibitory effect on the swimming ability of Daphnia magna.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.