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Biodegradation in water: screening tests

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Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
GLP compliance:
yes (incl. QA statement)
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
Municipal activated sludge from the wastewater treatment plant of the city Mannheim/Baden Württemberg (Germany). It was collected at 17 January 2006 from the aeration tank of the plant and aerated in the laboratory until use . Before use an appropriate aliquot of the activated sludge suspension was sieved with a finely woven mesh (mesh size about 0.8 mm), washed with tap water and pre-aerated for about 24 hours. The sludge of this suspension was adjusted to a concentration of 0.75 g/L of dry weight and then added to the test vessels to obtain a sludge concentration of 30 mg/L dry substance.
Duration of test (contact time):
28 d
Initial conc.:
98.8 - 101.6 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Reference substance:
aniline
Parameter:
% degradation (O2 consumption)
Value:
< 10
Sampling time:
28 d
Results with reference substance:
Degradation degree of the reference substance after 7 days: 50-60%
Degradation degree of the reference substance after 14 days: 60-70%
Interpretation of results:
other: poorly biodegradable
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
GLP compliance:
yes (incl. QA statement)
Specific details on test material used for the study:
- Batch No.of test material: 2005-01
- Appearance: black fine powder
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Activated sludge taken from a municipal waste water treatment plant of the city of Mannheim, Germany
- Initial cell/biomass concentration: 30 mg/l dry substance.
Duration of test (contact time):
28 d
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
TEST CONDITIONS
- Test temperature: 22 ± 1 °C
- pH: 7.5 - 7.8


TEST SYSTEM
- Culturing apparatus: Sapromat


CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes
- Toxicity control: yes
Reference substance:
aniline
Parameter:
% degradation (O2 consumption)
Value:
< 10
Sampling time:
28 d
Details on results:
DEGRADATION KINETIC (BOD/ThOD)
Time (d) Degradation (%)
7 -1
14 -2
21 -2
28 -2
Results with reference substance:
Biodegradation of aniline after 14 days: 60-70 %
Interpretation of results:
under test conditions no biodegradation observed
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal activated sludge from laboratory wastewater treatment plants fed with municipal sewage.
- Concentration of sludge: 30 mg/I
Duration of test (contact time):
28 d
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
TEST CONDITIONS
- Test temperature:
- pH: 7.2 - 7.5

TEST SYSTEM
- Number of culture flasks/concentration: 7

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes
- Toxicity control: yes
Reference substance:
aniline
Parameter:
% degradation (O2 consumption)
Value:
0 - 10
Sampling time:
28 d
Details on results:
- Degradation degree of the test substance after 28 days (% BOD/ThOD) : 0-10
- Degradation degree of the test substance at the end of the test (% BOD/ThOD) : 0-10
Results with reference substance:
- Degradation degree of the reference substance after 14 days (% BOD/ThOD) : 70-80
Interpretation of results:
other: poorly biodegradable
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
read-across based on grouping of substances (category approach)
Adequacy of study:
key study
Justification for type of information:
Please see the category read-across justification in the category object.
Parameter:
% degradation (O2 consumption)
Value:
< 10
Sampling time:
28 d

Description of key information

Poorly biodegradable

Key value for chemical safety assessment

Additional information

The substance belongs to the category of perylene as indicated by its chemical core structure. Based on this structure and the chemical and biological stability of perylenes under environmental conditions, data from structural analogue perylenes can be used to cover this endpoint required under REACh.

The biodegradability of the analogue substances (CAS 81 -33 -4; EC 479 -300 -2; EC 475 -310 -6) were tested in a guideline studies following OECD 301F. The biodegradation rate was determined by measuring the O2 consumption of domestic activated sludge. At test termination after 28 days a degradation rate of <10 % was determined, respectively [BASF AG 1999, BASF 2006].