isliFe

Long-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across: supporting information

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

628 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Key result

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

320 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Duration:

21 d

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

>= 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Validity criteria fulfilled:

yes

Conclusions:

In this reproduction study, FeNa-EDDHMA had no effect on the survival of Daphnia magna at concentrations up to and including 1000 mg/L. A biologically relevant effect on reproductive capacity of Daphnia magna was noted at 1000 mg/L, resulting in a NOEC and EC50 for reproduction of 320 mg/L and 628 mg/L, respectively

Based on the structural similarity, the similar physico-chemical properties and the similar toxicity to aquatic invertebrates and algae between FeNa-EDDHMA and MEAHA-Fe, identical conclusions are drawn for the read-across target MEAHA-Fe.

Executive summary:

The effects of the FeNa-EDDHMA on survival and reproductive Performance in Daphnia magna during 21 days of exposure were investigated in an OECD guideline No. 211 "Daphnia magna Reproduction Test".

The nominal concentrations tested were 10, 32, 100, 320 and 1000 mg/L. Duration of the study was 21 days and the test solutions were renewed three times a week. 10 neonate daphnia for the test concentrations and 20 neonate daphnia for the control group were investigated. At the start of the test and every workday, the condition of the parental daphnia was recorded and during the reproduction phase the number of living offspring, immobile young and the presence of unhatched eggs were recorded. During the study duplicate samples of 10 ml were taken from freshly prepared solutions on days 0, 7, 14 and 19, from 72-hour old solutions on day 3 and from 48-hour old solutions on day 21. Samples were stored in a deep-freeze until possible anaiysis.

Analysis of the samples showed that the method of preparation of test media provided repeatable recoveries of test substance concentration. Measured concentrations were all in agreement with nominal, except for those measured in the samples taken at 1000 mg/L (85% - 89%) during the 2 -day period after the last renewal. Further, the measured concentrations were stable during the periods between renewals of the test solutions.

No mortality or other visible effects were recorded for the parental organisms at any of the test item concentrations tested.During the total exposure period of 21 days, no significant (greater than 10%) immobilisation (including mortality) of newborn young was observed at any of the test concentrations.

In conclusion the FeNa-EDDHMA had no effect on the survival of Daphnia magna at concentrations up to and including 1000 mg/L in this reproduction study. The test item affected the reproductive capacity of Daphnia magna at concentrations of 320 and 1000 mg/L. A biologically relevant effect on reproductive capacity of Daphnia magna was noted at 1000 mg/L.

Based on the structural similarity, the similar physico-chemical properties and the similar toxicity to aquatic invertebrates and algae between FeNa-EDDHMA and MEAHA-Fe, identical conclusions are drawn for the read-across target MEAHA-Fe.

Description of key information

Read across to structural analogue FeNa-EDDHMA.

In this long-term study FeNa-EDDHMA had no effect on the survival of Daphnia magna at concentrations up to and including 1000 mg/L. A NOEC of 320 mg/L was derived based on reproduction and greater than or equal to 1000 mg/L based on parental mortality.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

320 mg/L

Additional information

Read across to structural analogue FeEDDHMANa. For justification of read across see IUCLID section 13.

The acute toxicity to Daphnia magna was performed according to OECD guideline 211. An EC50 of 628 mg/L based on reproduction and an EC50 of greater than 1000 mg/L based on parental mortality was detected. The animals were exposed to FeNa-EDDHMA for 21 days in a static test system (Akzo Nobel Chemicals B.V., 1996). A NOEC of 320 mg/L was derived based on reproduction and greater than or equal to 1000 mg/L based on parental mortality.