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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge: activated sludge form the sewage treatment plant in Schmallenberg, Germany. Mainly fed with municipal wastewater.
- Preparation of inoculum for exposure: The samples were washed once with mineral medium after the arrival at the laboratory and kept aerobic until use
- Concentration of sludge: 28.3 mg dry weight/L
Duration of test (contact time):
28 d
Initial conc.:
100 mg/L
Based on:
test mat.
Initial conc.:
3.05 other: mg O2/ mg test item
Based on:
ThOD
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
TEST CONDITIONS
- Test temperature: 22 °C
- pH: 7.8 -8.4

TEST SYSTEM
- Culturing apparatus: Sapromat respirometer
- Number of culture flasks/concentration:
- Test vessel: 2 replicates
- Inoculum blank: 2 replicates
- Abiotic contzrol: 2 replicates
- Procedural control: 2 replicates
- Toxicity control: 2 replicates
Reference substance:
other: sodium benzoate
Key result
Parameter:
% degradation (O2 consumption)
Value:
80
Sampling time:
28 d
Details on results:
Mean degradation kinetic (test substance):
12 % degradation after 3 d
44 % degradation after 7 d
54 % degradation after 10 d
60 % degradation after 12 d
72 % degradation after 20 d
76 % degradation after 24 d
80 % degradation after 28 d

Mean degradation kinetic (toxicity control):
14 % degradation after 1 day
30 % degradation after 7 days
40 % degradation after 14 days
56 % degradation after 28 days

Validation criteria:
- The differente of extremes of replicate values of the removal of the test item at the end of the test and at the end of the 10-d window is less than 20%,
- The percentage degradation of the reference item has reached the pass level of 60% by day 14,
- The oxygen uptake of the inoculum blank is < 60 mg/I in 28 days and the pH value is within the range 6.0 - 8.5.
Results with reference substance:
Degradation kinetic:
33 % degradation after 1 d
82 % degradation after 14 d
89 % degradation after 28 d
Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
Due to an biodegradation over 60 % within the 10-day-window, the test item can be identified as readily biodegradable.

Description of key information

Readily biodegradable: 80% biodegradation after 28 d (OECD 301 F)

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable
Type of water:
freshwater

Additional information

One experimental study is available investigating the ready biodegradability of the test substance. The study was performed according to OECD Guideline 301 F and GLP. Non-adapted activated sludge from a domestic sewage treatment plant was used as inoculum. After 28 days 80% degradation of the test substance was recorded based on O2-consumption. The 10-day window was met. Thus, the test substance is readily biodegradable according to the OECD criteria. A toxicity control containing both, reference substance and test substance did not indicate inhibitory effects to the inoculum (40% degradation after 14 d). All validity criteria were met.