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EC number: 812-724-1
CAS number: 106705-37-7
Table 1: Results after 240 minutes
Opacity value = Difference (t240 – t0) of opacity
Permeability at 490 nm (OD490)
In vitro irritancy score
Mean in vitro irritancy score
the negative control (saline) neither an increase of opacity nor
permeability of the corneae could be observed (mean IVIS = 1.69).
The positive control (10% (w/v) Benzalkonium chloride in saline) showed
clear opacity of the corneae (mean IVIS =124.792).
Table 2: Historical data
Range of IVIS
62.60 - 292.29
0.00 – 3.00
206 studies with solid test items performed from February 2007 until
December 2014 based on 0.5% sodium fluorescein solution in HBSS (w/v)
and sodium fluorescein solution of 200 µL per well
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