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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
26 - 28 January 1993
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study in principle according to guideline and under GLP. No data on actual concentrations and hardness of water too low.
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
yes
Remarks:
No chemical analysis, water hardness less than adviced
Principles of method if other than guideline:
No measurement of actual concentration, hardness below recommended concentration (50 iso 140 mg/L as CaCO3). 2 reps per group iso 4. Statistics according to Thompson and not Finney.
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
Direct dispersion in water. 200 mg of test material was dispersed in aged and dechlorinated tap water and the volume adjusted to 2 litres to give a 100 mg/L test concentration from which dilutions are made to give the test series.

Test organisms (species):
Daphnia magna
Details on test organisms:
Name: Daphnia magna Straus
Source: Laboratory culture originating from a strain supplied by the Institut national de Recherche Chimique Appliquée (IRCHA), France
Culture: At 21C in propylene vessels containing two litres of dechlorinated and aged tap water. Cultures were fed daily with a suspension of mixed algae (predominantly Chlorella spp.). Culture conditions ensure that reproduction is by parthogenesis. Gravid adults were isolated 24 hours prior to the initiation of the test. Young daphnids produced overnight were used for testing.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
None
Hardness:
50 mg/L as CaCO3
Test temperature:
22.0 C
pH:
7.5 - 7.7
Dissolved oxygen:
8.0 - 8.7 mgO2/L
Nominal and measured concentrations:
Nominal concentrations:
1.0, 1.8, 3.2, 5.6, 10, 18, 32, 56, 100 mg/L
Details on test conditions:
The test material was suspected to absorb to glassware and so saturation of the absorption sites was achieved by soaking the test vessels overnight prior to the start of the test with the test solutions. At 0 hours the test vessels were emptied, rinsed with the solution to be tested and then refilled with the fresh test solution.
Test vessels were glass jars each containing 200 ml test solution and were covered to reduce evaporation. 1 control in duplicate and 9 test concentrations in duplicate. 20 mL test solution per organism, 10 per replicate. No aeration during the test and a 16h light - 8h dark photoperiod. Daphnia were placed in the test solutions after addition of the test material. Daphnia were considered to be immobilised if they were unable to swim for approximately 15 seconds after gentle agitation.
Reference substance (positive control):
no
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
1.56 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mobility
Remarks on result:
other: 95% CL: 1.38 - 1.77 mg/L
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% CL: 9.0 - 12 mg/L
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
5.2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% CL: 4.6 - 5.9 mg/L
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
3.2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Details on results:
Concentrations >= 18 mg/L caused complete immobility within 24 hours. No immobilisation was observed up to concentrations of 3.2 mg/L during the study period. At the dose rate of 5.6 mg/L no immobilisation was apparent after 24 hours but 13/20 animals were immobilised after 48 hours. In the 10 mg/L dose group 9/20 animals were immobilised after 24 hours, whilst all animals were immobilised after 48 hours.
The EC50 24h and EC50 48h were determined at 10 and 5.2 mg/L respectively on a nominal basis.
The 48h NOEC was concluded at 3.2 mg/L nominally.
Reported statistics and error estimates:
Analysis of the immobility data by the moving average method of Thompson (1947).

Number of mobile animals

Conc substance

Test vessel

in mg/l

number

0 hours

24 hours

48 hours

control

1

10

10

10

2

10

10

10

total

20

20

20

1.0

1

10

10

10

2

10

10

10

total

20

20

20

1.8

1

10

10

10

2

10

10

10

total

20

20

20

3.2

1

10

10

10

2

10

10

10

total

20

20

20

5.6

1

10

10

4

2

10

10

3

total

20

20

7

10

1

10

6

0

2

10

5

0

total

20

11

0

18

1

10

0

0

2

10

0

0

total

20

0

0

32

1

10

0

0

2

10

0

0

total

20

0

0

56

1

10

0

0

2

10

0

0

total

20

0

0

100

1

10

0

0

2

10

0

0

total

20

0

0

Validity criteria fulfilled:
yes
Conclusions:
Unfortunately no preliminary test was done. Instead of nine test concentrations, four would have been sufficient for the same amount of information.
Concentrations >= 18 mg/L caused complete immobility within 24 hours. No immobilisation was observed up to concentrations of 3.2 mg/L during the study period. At the dose rate of 5.6 mg/L no immobilisation was apparent after 24 hours but 13/20 animals were immobilised after 48 hours. In the 10 mg/L dose group 9/20 animals were immobilised after 24 hours, whilst all animals were immobilised after 48 hours. The EC50 24h and EC50 48h were determined at 10 and 5.2 mg/L respectively on a nominal basis. The 48h NOEC was concluded at 3.2 mg/L nominally. Expressed for active ingredient (30%) this would give EC50 values of 3.1, 1.6 and a NOEC of 0.96 mgai/L.
Executive summary:

In this study the acute toxicity of Ampholak YCE to Daphnia magna was studied according to OECD202. The test was performed under GLP as a static test. Each replicate contained 10 daphnids, 2 replicates for control and for the nine test concentrations. Concentrations >= 18 mg/L caused complete immobility within 24 hours. No immobilisation was observed up to concentrations of 3.2 mg/L during the study period. At the dose rate of 5.6 mg/L no immobilisation was apparent after 24 hours but 13/20 animals were immobilised after 48 hours. In the 10 mg/L dose group 9/20 animals were immobilised after 24 hours, whilst all animals were immobilised after 48 hours. The EC50 24h and EC50 48h were determined at 10 and 5.2 mg/L respectively on a nominal basis. The 48h NOEC was concluded at 3.2 mg/L nominally.

Description of key information

One acute daphnia study with Sodium cocopropylenediamine propionate is available. The study lacks the quantification of the test substance during the test. The study from Sewell and Grant-Salmon (1993) resulted in a 48h EC50 for daphnia magna of 1.6 mg a.i./L based on nominal test concentrations.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
1.6 mg/L

Additional information

One acute daphnia study with Sodium cocopropylenediamine propionate is available. The study lacks similar to the fish study the quantification of the test substance during the test. When these studies were performed no suitable sensitive analytical method was available for the quantification of this test substance. The study from Sewell and Grant-Salmon (1993) resulted in a 48h EC50 for daphnia magna of 1.6 mg a.i./L based on nominal test concentrations.