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EC number: 266-767-0 | CAS number: 67601-05-2
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test solutions were prepared following OECD guideline 23. A saturated stock solution was prepared. - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Source (laboratory, culture collection): Collection of algal cultures in University of Goettingen, Germany - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 23 - 23.2 °C
- pH:
- 7.9 - 8.4
- Nominal and measured concentrations:
- Nominal test concentrations (mg/l):
0, 1, 3.2, 10, 32, 100
Measured test concentration (mg/l):
at test start: n.d., nd. , 0.67, 2.22, 7.20
at 72 h: all test concentrations below detection limit - Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flasks (250 ml), gas permeable silicon sponge caps
- Fill volume: 100 ml
- Initial cells density: 50000
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to the OECD guideline 201
OTHER TEST CONDITIONS
- Photoperiod: permanent illumination
- Light intensity and quality: universal white, average 5375 lux, wave length 400 - 700 nm
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Chlorophyll measurement: chlorophyll-a-fluorescence at a wave length of 432 nm at 0, 24, 48 and 72 h.
TEST CONCENTRATIONS
- Range finding study: EC50 fluorescence: < 10 mg/l, ErC50. 13.5 mg/l - Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 2.22 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 7.2 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Conclusions:
- The acute toxicity of citronellyl acetate to Desmodemus subspicatus was tested in a guideline study according to OECD 201. At test termination after 72 hours an EC50 > 7.2 mg/l based on algal growth rate was detected. The concentrations was analytically determined (BASF SE, 2010).
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
- Justification for type of information:
- See read across justification document attached in section 13.2
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test solutions were prepared following OECD guideline 23. A saturated stock solution was prepared. - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Source (laboratory, culture collection): Collection of algal cultures in University of Goettingen, Germany - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 23 - 23.2 °C
- pH:
- 7.9 - 8.4
- Nominal and measured concentrations:
- Nominal test concentrations (mg/l):
0, 1, 3.2, 10, 32, 100
Measured test concentration (mg/l):
at test start: n.d., nd. , 0.67, 2.22, 7.20
at 72 h: all test concentrations below detection limit - Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flasks (250 ml), gas permeable silicon sponge caps
- Fill volume: 100 ml
- Initial cells density: 50000
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to the OECD guideline 201
OTHER TEST CONDITIONS
- Photoperiod: permanent illumination
- Light intensity and quality: universal white, average 5375 lux, wave length 400 - 700 nm
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Chlorophyll measurement: chlorophyll-a-fluorescence at a wave length of 432 nm at 0, 24, 48 and 72 h.
TEST CONCENTRATIONS
- Range finding study: EC50 fluorescence: < 10 mg/l, ErC50. 13.5 mg/l - Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 2.22 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 7.2 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Conclusions:
- The acute toxicity of citronellyl acetate to Desmodemus subspicatus was tested in a guideline study according to OECD 201. At test termination after 72 hours an EC50 > 7.2 mg/l based on algal growth rate was detected. The concentrations was analytically determined (BASF SE, 2010).
Referenceopen allclose all
Description of key information
Acutely toxic for aquatic organisms
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 7.2 mg/L
- EC10 or NOEC for freshwater algae:
- 2.22 mg/L
Additional information
The acute toxicity of citronellyl acetate to Desmodemus subspicatus was tested in a guideline study according to OECD 201. At test termination after 72 hours an EC50 > 7.2 mg/l based on algal growth rate was detected. The concentrations was analytically determined (BASF SE, 2010).
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