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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

Assessing the ready biodegradability of the substance as study was conducted in accordance with the EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test) which is in all essential parts identical with OECD Guideline 301 F. A solution of the test substance in a mineral medium was inoculated and incubated for 28 d under aerobic conditions. During this period, degradation was followed by BOD determinations. The test substance showed 47 % biodegradation after 28 days.


The inherent biodegradability was determined according to the OECD Guideline 302 C (Inherent Biodegradability: Modified MITI Test (II)) and results in 78 % degradation within 28 days.


Based on these results, the substance is considered to be "Not Readily Biodegradable", but "Inherently Biodegradable. However, based on the substance properties, i.e. poor water solubility, the registrant considers repeating the ready biodegradability test following the conditions for poorly soluble substances according to annex III of OECD TG 301 (1992).

Key value for chemical safety assessment

Biodegradation in water:
inherently biodegradable

Additional information

Assessing the ready biodegradability of the substance as study was conducted in accordance with the EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test) which is in all essential parts identical with OECD Guideline 301 F. A solution of the test substance in a mineral medium was inoculated and incubated for 28 d under aerobic conditions. During this period, degradation was followed by BOD determinations. The test substance showed 47 % biodegradation after 28 days.


The inherent biodegradability was determined according to the OECD Guideline 302 C (Inherent Biodegradability: Modified MITI Test (II)) and results in 78 % degradation within 28 days.


Based on these results, the substance is considered to be "Not Readily Biodegradable", but "Inherently Biodegradable.