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Biodegradation in water: screening tests

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Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2012
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Reference substance:
benzoic acid, sodium salt
Parameter:
% degradation (CO2 evolution)
Value:
83.9
Sampling time:
28 d
Remarks on result:
other: mean of 3 replicates
Interpretation of results:
readily biodegradable
Conclusions:
In a study performed according to OECD 301B, SLMI is readily biodegradable (83.9% in 28d) and passes the 10 day window.
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
Qualifier:
according to
Guideline:
EPA OPPTS 835.3110 (Ready Biodegradability)
GLP compliance:
yes (incl. certificate)
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Details on inoculum:
- Source of inoculum/activated sludge : Brazos River Authority Sewage Treatment Plant Sugar Land, TX
- Storage conditions: Sample was aerated during transport and upon arrival at the laboratory
Duration of test (contact time):
ca. 28 d
Initial conc.:
>= 10 - <= 20 mg/L
Based on:
other: TOC/L
Details on study design:
TEST CONDITIONS
- Composition of medium: 1 L using deionised water 33.0 g Na2HPO4 2H2O, 8.5g KH2PO4, 21.75g K2HPO4, 0.5g NH4Cl, 36.4g CaCl 2H2O, 22.5g MgSO4 7H2O, 0.25 Fe Cl3 6H2O
- Additional substrate:
- Test temperature: 20-25º C
- pH: 7.4±0.5
- pH adjusted: yes
- Aeration of dilution water: CO2 free air was pumped throgh the flasks continually
- Continuous darkness: yes



TEST SYSTEM
- Culturing apparatus: Glass flasks
- Number of culture flasks/concentration: 2 flasks for each blank, teference and test flask. (8 flasks total)
- Method used to create aerobic conditions: CO2 free air was pumped continously through the flasks
- Test performed in open system: No
- Details of trap for CO2 : 160 ml square bottles containing 100 ml of 0.0125 M Ba(OH)2 were attached to the test flasks.


SAMPLING
- Sampling frequency: Samples were taken on days 2, 3, 4, 5, 6, 7, 8, 9, 11, 12, 14, 15, 19, 25, 26, and 27.
- Sampling method: The Ba(OH)2 was titrated against 0.05NHCl to measure the amount of CO2 evolved. on Day 27 ll test flasks were acidified using NHCl The flasks were then aerated for a further 24 hours. All remaining Ba(OH)2 was then removed for titration on day 28.


Reference substance:
aniline
Parameter:
% degradation (CO2 evolution)
Value:
ca. 90.4
Sampling time:
28 d
Parameter:
% degradation (CO2 evolution)
Value:
ca. 69.34
Sampling time:
12 d
Remarks on result:
other: This was the end of the 10 day window started on day 2. As SCMI produced CO2 equal to more than 60% of its CO2 then it can be considered readily biodegradable
Results with reference substance:
The results for distilled aniline showed that on day 15 the mean biodegradation was 73.52% therefore the test was considered valid.
Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable

Description of key information

In a study performed according to OECD 301B, SLMI is readily biodegradable and passes the 10 day window.


In a study on read-across substance SCMI (Rodrigue, 2007) showed Ready Biodegradability passing the 10 day window.

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

Two key studies have been performed to test the ready biodegradability following the OECD 301B CO2 evolution test methods. The first study was performed on SLMI and showed biodegradability of >80% in 28days and passed the 10 day window.

The second study was performed on the read-across substance SCMI (Rodrigue. 2007) and measured biodegradation of >60% at the end of 10 days, showing ready biodegradation of the substance.