[No public or meaningful name is available]

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

Cited as Directive 92/69/EEC, C.1

Deviations:

yes

Remarks:

According to the demands of the used guideline the test was performed only with 7 fish per concentration.

Principles of method if other than guideline:

In most parts equivalent to the OECD Guideline for Testing of Chemicals No. 203 'Fish, Acute Toxicity Test' (1992).

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

PRETREATMENT OF TEST ITEM:
- 501.9 mg of the test item were added to 5 litres of dilution water
- treated for 60 seconds at 8000 rpm with an ultra turrax, stirred for 4 hours on a magnetic stirrer
- filtration by using an aseptic filter of pore size 0.2 μm

Test organisms (species):

other: Brachydanio rerio HAMILTON BUCHANAN (new scientific name: Danio rerio)

Details on test organisms:

- Source: Aqua KloeGer (Germany)
- Date of birth: 2007, April/May
- Date supplied: 2007-09-18
- Acclimatisation: Stock held since 2007-09-18 and acclimatised to the test conditions since then.
- Temperature: 20-24 °C
- Dissolved oxygen: > 5 mg/L
- Feeding: Commercial fish food, daily. Discontinued 24 h prior to test.
- Mortalities during acclimatisation period: < 5 %
- Medication: none
- Mean standard length (n = 14): 3.8 cm (S.D. = 0.17 cm)
- Holding and dilution water: Reconstituted water prepared according to the recommendations of ISO 7346.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

13.6 °dH (243 mg/L CaCO3)

Test temperature:

22.3 °C after 96 hours

pH:

pH value: 7.2 after 96 hours

Dissolved oxygen:

oxygen (mg/l): 7.6 after 96 hours

Salinity:

no data

Nominal and measured concentrations:

Nominal concentration of test substance: 100 mg/l (HPLC mean value after 96 h: 85.5 mg/l). This value was determined three days later after storing at room temperature, because of a defect of the HPLC apparatus. As the recovery was > 80 % this has no impact on the study result.

Details on test conditions:

TEST SYSTEM:
- keeping water: synthetic fresh water according to ISO 7346
- photoperiod: 16 hours light, 8 hours dark
- ventilated
- no feeding during test
- test vessel: glass aquarium (300 x 135 x 200 mm), 5 l test media
- number of fish per test vessel: 7
- method of administration: direct weighing
- test concentration: 100 mg/l plus one control
- test parameter: mortality (recorded daily)

Reference substance (positive control):

not required

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

72 h

Dose descriptor:

LC0

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

2 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Recovery rates ranged from 100.5 – 102.5 % of nominal values at 0 hours and from 85.3 – 85.8 % of nominal values at 96 hours, respectively.

Validity criteria fulfilled:

yes

Conclusions:

 For fish (Danio rerio) the acute toxicity of the test item was >= 100 mg/l (96 h-LC0).

Executive summary:

 For fish (Danio rerio) the acute toxicity of the test item was >= 100 mg/l (96 h-LC0). The study was conducted according to Directive 92/69/EEC, C1. The result relates to nominal concentration as analytical monitoring shows stability of the test substance during the experiment (Currenta, 2008).

 

Description of key information

For fish (Danio rerio) the acute toxicity of the test substance was >= 100 mg/l (96 h-LC0). The study was conducted according to Directive 92/69/EEC, C1. The result relates to nominal concentration as analytical monitoring shows stability of the test substance during the experiment (Currenta, 2008).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L