2,2'-(C12-14 evennumbered alkyl imino) diethanol

Endpoint summary

Description of key information

The toxic effect of the test item 2,2’-(C12-14 evennumbered alkylimino) diethanol to Daphnia magna was assessed in a semi-static concentration-response test. The 48-hour NOEC was calculated to be 449 μg test item/L. The 48-hour LOEC was calculated to be 1064 μg test item/L and the 48-hour EC50 value was calculated to be 910 μg test item/L.
The initial concentrations and the maintenance of the exposure concentrations during the test were verified in the analytical part. All reported results refer to time-weighted average concentrations of the test item (weighted sum of time-weighted average values of both measured main ingredients) since the concentrations of the main ingredients were not within ± 20% of the nominal concentrations during the test for all test concentrations and different recovery decrease was determinable for each ingredient.

 

The influence of 2,2’-(C12-14 evennumbered alkylimino)diethanol on the growth of the freshwater green algae Pseudokirchneriella subcapitata was assessed in a static concentration-response test. The 72-hour EyC50 was calculated to be 3.51 μg test item/L (initial mean: 5.02 μg test item/L) and the 72-hour ErC50 value was calculated to be 6.13 μg test item/L (initial mean: 37.2 μg test item/L). The 72-hour NOEyC was determined to be 2.13 μg test item/L (initial mean: 1.97 μg test item/L) and the associated 72-hour LOEyC was 3.92 μg test item/L (initial mean: 5.47 μg test item/L). The 72-hour NOErC was determined to be 2.13 μg test item/L (initial mean: 1.97 μg test item/L) and the associated 72-hour LOErC was 3.92 μg test item/L (initial mean: 5.47 μg test item/L).

The initial concentrations and the maintenance of the exposure concentrations during the test were assessed in the analytical part. All reported results refer to geometric mean measured concentrations of the test item (weighted sum of geometric mean values of both measured main ingredients) since the concentrations of the main ingredients were not within ± 20% of the nominal concentrations during the test for all test concentrations and different recovery decrease was determinable for each ingredient. In addition, all results were evaluated based on initial mean measured concentrations to compensate varying intermediate recoveries that were determined and to reflect actual dosage of the test item.

In conclusion, the effect concentrations based on inital mean measured concentrations are more realistic to derive the toxicity of the registration substance as they reflect the dissolved amount of substance in combination with the still bioavailable amount adsorbed to algae cells.

The registration substance is readily biodegradable.

From the available data the following worst case CLP-classification is derived:

Aquatic acute 1, M1 ; Aquatic chronic 1, M10