trans-1-(1-oxohexadecyl)-4-[(1-oxohexadecyl)oxy]-L-proline

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2008 june to 2008 december

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Melting point: 69°C
- Flash point: 243.5°C
- Vapour pressure: no data
- Water solubility (under test conditions): <1mg/L
- Henry's law constant: no data
- log Pow: 13.2

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: daphnia magna straus, clone number 5
- Age at study initiation (mean and range, SD): 24hours
- Breeding: the daphnids breeding is carried out in closed bottles placed in a climatic chamber free from any toxic vapor
- Feeding during test
- Food type: no feed during the study

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

observation of the mobility means the inability fo the daphnids to swim

Hardness:

250mg/L of CaCO3

Test temperature:

19.6-20°C

pH:

7.7

Dissolved oxygen:

8.6

Salinity:

CaCL2, 2H2O=297mg/L
MgCL2, 6H2O=187mg/L
NaHCO3=200mg/L
K2SO4=26mg/L

Nominal and measured concentrations:

1; 10; 100 mg/L

Details on test conditions:

TEST SYSTEM

- No. of organisms per vessel:5
- No. of vessels per concentration (replicates):4
- No. of vessels per control (replicates):4
- No. of vessels per vehicle control (replicates):4

Reference substance (positive control):

yes

Remarks:

Dichromate potassium

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

ca. 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

ca. 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Details on results:

- Other biological observations:no immobilistaion observed after every 24 hours of exposure

Results with reference substance (positive control):

EC50-24 = 1.12 mg/L (1.00-1.26)

The acute immobilisation screening test of the micro crustacean Daphnia magna was performed on the test item "LCA07033". the results obtained on the toxicological endpoint "immobilisation" gives an EC50 -48hours superior to the highest tested concentration , i.e 100mg/L.

Analyses of the test solutions were performed: the dipalmitoyl Hydroxyproline was analysed, but the concentrations measured in the test solutions were lower than the Limit Of Detection.

Validity criteria fulfilled:

yes

Conclusions:

EC50-48hours of the test item "LC07033" on the cladocera micro crustacean Daphnia magna is superior to the highest tested concentration , i.e 100mg/L, value expressed in nominal concentration.

Executive summary:

The acute immobilisation screening test of the micro crustacean Daphnia magna was performed on the test item "LCA07033". the results obtained on the toxicological endpoint "immobilisation" gives an EC50 -48hours superior to the highest tested concentration , i.e 100mg/L.

Analyses of the test solutions were performed: the dipalmitoyl Hydroxyproline was analysed, but the concentrations measured in the test solutions were lower than the Limit Of Detection.

Description of key information

EC50 > 100 mg/L

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

100 mg/L