Reaction product of "Fatty acids, C16-18 (even numbered) and C18-unsaturated" and "reaction mass of 1,3-alkanediol, 2-(hydroxymethyl)-2-[(methoxymethoxy)methyl]- and 1,3-heteromonocycle-5,5-dimethanol

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

September 06-09, 2004

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study has been performed according to ISO guidelines and according to GLP principles.

Principles of method if other than guideline:

ISO 14669 (1998): Water Quality - Determination of acute
lethal toxicity to marine copepods (Copepoda, Crustacea).


OECD 2000: Guidance Document on aquatic toxicity testing of
difficult substances and mixtures. OECD Environmental Health
and Safety Publications Series on Testing and Assessment
No. 23. ENV/JM/MONO(2000)6.


ISO 8245: Water Quality - Guidelines for the determination
of total organic carbon (TOC) and dissolved organic carbon
(DOC).

GLP compliance:

yes

Analytical monitoring:

yes

Details on test solutions:

Identity and concentration of auxiliary solvent for dispersal: Species: the marine crustacean Acartia tonsa.

Test medium: natural seawater (salinity adjusted by
distilled water to 32 S).

Reference substance: 3,5-dichlorophenol


Separate WAF's were prepared for 5 loading rates (101-180-
321-561-1000 mg/l) in the dark at 21°C. After 23 h WAF's
were filtered through Wathman GF/C glass fibre filters to
remove non-settling suspended material.

Test organisms (species):

other aquatic crustacea: Acartia tonsa

Test type:

static

Water media type:

saltwater

Limit test:

no

Total exposure duration:

48 h

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 34.8 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

other: TOC

Basis for effect:

mortality

Remarks on result:

other: WAF loading was 1000 mg/l

Comments:
TOC analysis:

control seawater: 1.0 mg TOC/l

101 mg/L WAF: 4.8 mg TOC/l

1000 mg/l WAF: 34.8 mg TOC/l

The results show that a portion of the test substance
partitioned to the water phase as dissolved organic matter.
Assuming that the carbon content of the test substance is
70% by weight, approximately 5% of the added substance was
present in the WAF's.

The acute lethality was less than 10% at loading rates up to
1000 mg/l.

Validity criteria fulfilled:

yes

Conclusions:

The 48 hour EC50 of Radiagreen EBL (Pentaerythritol mono oleate base Polyol) is estimated as greater than 1000 mg/l. The highest no-observed (lethal) effect concentration (NOEC) was estimated as 1000 mg/l.

Description of key information

The study was performed according to ISO Guideline 14669 and GLP principles.

Key value for chemical safety assessment

Marine water invertebrates

Marine water invertebrates

Effect concentration:

34.8 mg/L

Additional information

The acute toxicity of Radiagreen EBL (Radia 7853) to the marine crustacean Acartia tonsa has been investigated. The test was performed according to ISO 14669: Determination of acute lethal toxicity to marine copepods (Copepoda, Crustacea). The test was performed on water accommodated fractions (WAF) of the test substance. The loading rates were 100 – 1000 mg/l. The Acartia tonsa were exposed for 48 hours at 20 ±1 °C. Mortality was recorded after 24 and 48 hours. The acute lethality of the substance on the marine copepod Acratia tonsa was less than 10% at loading rates up to 1000 mg/l in acoomodated fractions. Based on analysis of TOC, this results in a 48 -h EC50 of > 34.8 mg/l, and therefore also beyond the water solubility limit.