Reaction product of "Fatty acids, C16-18 (even numbered) and C18-unsaturated" and "reaction mass of 1,3-alkanediol, 2-(hydroxymethyl)-2-[(methoxymethoxy)methyl]- and 1,3-heteromonocycle-5,5-dimethanol

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

September 20-25, 2004

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study has been performed according to OECD guidelines and according to GLP principles.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

The test substance nominal concentrations were prepared as WAF's according to OSPAR/PARCOM Protocols on Methods for the Testing of Chemicals Used in the Offshore Industry, 1995.

GLP compliance:

yes

Analytical monitoring:

no

Details on test solutions:

Identity and concentration of auxiliary solvent for dispersal: A nominal 1000 mg/l solution was prepared in standardised
artificial seawater (salinity 32-35 g/l NaCl), shaken
vigorously and allowed to stand for 4 h. A cloudy off-white
solution with floating material was observed. For each
nominal concentration (560, 1000 and 1800 mg/l) the required
amount of homogenised sample was added to 12 l dilution
water, mixed for 20-24 h and then allowed to separate for 4
h. The subnatant liquid was drawn off and used for the test
(water accommodated fractions).

Test organisms (species):

other: Turbot (Scophthalmus maximus)

Test type:

semi-static

Water media type:

saltwater

Limit test:

no

Total exposure duration:

96 h

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 800 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

Comments:
There was no chemical analytical confirmation of the actual
dissolved concentrations in this saltwater fish study . The
dissolved concentrations were likely to be lower than the
nominal concentrations as the test substance was poorly
soluble in water.

Conclusions:

The 96 hour LC50 of Radiagreen EBL Pentaerythritol fatty acid ester is estimated as greater than 1800 mg/l. The highest no-observed (lethal) effect concentration (NOEC) was estimated as 1800 mg/l.

Description of key information

The study was performed based on OECD Guideline 203 and GLP principles.

Key value for chemical safety assessment

Marine water fish

Marine water fish

Effect concentration:

1 800 mg/L

Additional information

A semi-static test was performed according to OECD 203 modified to marine conditions. The Turbot (Scophthalmus maximus) was exposed to 560, 1000 and 1800 mg/l, although this is above the water solubility limit and no analytical measurements have been performed. The substance induced no visible effects in turbot up to the highest concentration tested. The 96h-LC50 for marine species was determined to be >1800 mg/L and therefore also beyond the water solubility limit.