Tetrabromophthalic anhydride

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1974

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The study was performed prior to international gudielines and good laboratory practices. It used a micronized form of the commerical product. Micronization may impact (e.g. increase) the potential for toxicity.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1974

Materials and methods

Principles of method if other than guideline:

Rats were exposed to a single concentration of the test material for 4 hours and observed for 14 days afterward.

GLP compliance:

no

Test type:

fixed concentration procedure

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Tetrabromophthalic anhydride

EC Number:

211-185-4

EC Name:

Tetrabromophthalic anhydride

Cas Number:

632-79-1

Molecular formula:

C8Br4O3

IUPAC Name:

tetrabromo-1,3-dihydro-2-benzofuran-1,3-dione

Details on test material:

A micronized form of the test material was used.

Test animals

Species:

rat

Strain:

other: Spartan

Sex:

male/female

Details on test animals or test system and environmental conditions:

Five male and five female rats were used. The rats were housed by sex in groups of 5 in metal cages above the droppings in temperature and humnidity contolled quarters. Purina Laboratory Chow and water were available ad libitum.

Administration / exposure

Route of administration:

inhalation: dust

Type of inhalation exposure:

whole body

Vehicle:

other: unchanged (no vehicle)

Details on inhalation exposure:

"The group of 10 rats was placed in a sealed 59.1 liter glass chamber and exposed for 4 hours to a dynamic atmosphere containing the dust of the test material. In order to prevent "piling up" during the exposure, the rats were separated into 4 units of 2 or 3 rats each. Addition of the test compound to the test chamber atmosphere was controlled by a Wright Dust Feeder. Dried and filtered air was passed through the mechanism and directly into the exposure chamber. Airflow was regulated by means of a flowmeter (Gelman Insturment Company, Ann Arbor, Michigan, Model No. 8221). The calculated atmospheric concentration administered was approximately 10.92 mg/L* of FM PHT4 (micronized), Lot N0. 6332-B.
*The physical properties of the test compound precluded administration of the test material at a higher atmospheric concentration."

Duration of exposure:

4 h

Concentrations:

10.92 mg/L

No. of animals per sex per dose:

5M/5F

Control animals:

no

Details on study design:

The rats were observed continuously during and immediately after the 4 hr exposure. All rats which died on study were necropsied. All surviving animals were necropsied at the end of the 14 d observation period.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 10.92 mg/L air (nominal)

Based on:

test mat.

Exp. duration:

4 h

Mortality:

None.

Clinical signs:

other: During exposure: decreased activity, eye squint, slight dyspnea, erythema. At 24 hr: 1 rat with nasal "porphyrin" discharge. At 10-14 days: several rats with diarrhea.

Body weight:

All rats exhibited normal body weight gain during the study.

Gross pathology:

No compound-related gross lesions were observed.

Applicant's summary and conclusion

Interpretation of results:

practically nontoxic

Remarks:

Migrated information Criteria used for interpretation of results: expert judgment

Conclusions:

The 4 hr LC50 in rats was > 10.92 mg/L.