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EC number: 701-390-1
CAS number: -
acute toxicity of Triameen Y12D to the fresh water fish Brachydanio
rerio (zebra fish) was tested under semi-static conditions in
accordance with OECD and EEC guidelines for testing of chemicals. The
test was performed in
compliance with Good Laboratory Practice (GLP). The fish were
exposed to the following nominal test concentrations: 0.1- 0.18 – 0.32 –
0.58 and 1.05 mg/l. The LC50(96 h) was calculated to be 0.43
mg/l with 95% confidence limits of 0.346 and 0.538 mg/l. The highest
concentration causing no mortality (no observed effect concentration,
NOEC) after 96 h amounted to 0.18 mg/l whereas 0.32 mg/l was the lowest
tested concentration (LOEC, 96 h) at which lethality was observed as
compared to the control. The LC100was 1.05 mg/l, which was
observed after 29 hours.
concentrations where lethality was established, sublethal effects, such
as reduced activity, deviations in the behavior or the appearance of the
fish, were observed. For ethical reasons two fish were euthanised at the
concentration of 0.58 mg/l after 53 hours.
of the applied concentrations was not possible due to the absence of a
suitable analytical method. The currently available analytical method is
capable of analyzing down to only 10 mg/l. Therefore only the two stock
solutions used to prepare the test concentrations were analyzed. These
analyses showed that the stock solutions were stable even after storage
for almost 7 months in the refrigerator. All concentrations mentioned in
this report are therefore based on nominal concentrations. Considering
the high water solubility of the test substance and its stability it is
reasonable to assume that the actual concentrations were identical to
the nominal concentrations during the entire test.
Only a limit number of short-term toxicity to fish studies are available
for the substances under consideration. Therefore the test results for
dodecyltriamine Y was added to the dossier. Tri- and tetramines contain
respectively about 22 – 11% diamines and for this reason also the data
for the diamines was added to the overview.
It is considered reasonable to add dodecyltriamine Y as a worst-case as
this substance is similar as the linear triamine and considered a the
most toxic triamine. Dodecyltriamine Y is therefore selected as the Key
study as this study is a Klimisch 1. The LC50 of this study is therefore
used as the Key value for chemical safety assessment as a reasonable
worst-case. In the final risk assessment this value will not be critical
as two chronic end points are available.
Most of the short-term
toxicity fish tests were conducted in a period when no reliable specific
method of analyses was available. The concentrations were therefore not
analytically verified, the reliability of the results is limited because
of the poor solubility of the test substances and partial sorption onto
the walls of test vessels. Hence, most of the tests were considered to
be valid with restrictions. Only one acute fish test study is available
(2372-82-9; dodecyltriamine Y)
which is used as a biocide and
this substance is considered as the most ecotoxic substance for the
alkyl polyamines (>2N).The
observed LC50 for this substance of 0.43 mg/L, suggests that the
alkyl-1,3-diaminopropanes (2N) are more ecotoxic than the alkyl
polyamines (>2N). Tri- and
tetramines contain respectively about 22 – 11% diamines and for this
reason the data for the diamines was added to the overview.
For oleyl tetramine (4N) an LC50 (96 h) has been
observed of 0.13 mg/L. This value is slightly lower than the value
observed for dodecyltriamine Y. In the presence of humic acid however
the toxicity of oleyl tetramine is reduced to an LC50 (96 h) of 2.01
mg/L demonstrating that under environmental conditions the toxicity is
significantly reduced by river water constituents.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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