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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
(Q)SAR
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification
Justification for type of information:
QSAR prediction: US EPA accepted QSAR method for chemicals properties assessment.
Qualifier:
according to
Guideline:
other: QSAR model
Principles of method if other than guideline:
EPI Suite v 4.0/ECOSARv1.00
GLP compliance:
no
Remarks:
not applicable. QSAR model
Analytical monitoring:
not required
Vehicle:
no
Test organisms (species):
other: Daphnid species
Test type:
other: QSAR model
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
not applicable. QSAR model
Test temperature:
not applicable. QSAR model
pH:
not applicable. QSAR model
Dissolved oxygen:
not applicable. QSAR model
Salinity:
not applicable. QSAR model
Nominal and measured concentrations:
not applicable. QSAR model
Details on test conditions:
100% Sodium ethyl xanthate (CAS No. 140-90-9)
SMILES : S=C([S-])OCC.[Na+]
CHEM : Carbonodithioic acid, o-ethyl ester, sodium salt
MOL FOR: C3 H5 O1 S2 Na1
MOL WT : 144.18
Log Kow: 0.87 (used)
Wat Sol: 1.79E+004 mg/L (WskowWin estimate)
Reference substance (positive control):
not required
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
445.776 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
The ECOSAR QSAR model predicts that LC50 for fresh water species cited would to be 445.776 mg/l therefore that the substance is effectively not toxic to Daphnid.

Overview of QSARs estimations on short-term aquatic toxicity of

Reaction mass of SEX

to Daphnid     

Method

 

Results

Remarks

Reference

QSAR

estimate

Daphnid , 48-hr LC50 = 445.776 mg/L (ppm)

 

Daphnid, 30-day, ChV (chronic value) = 43.190 mg/L (ppm)

 

 

Reliable with restrictions,

QSAR estimated data

ECOSAR Program (v1.00)

 

 

The ECOSAR QSAR model predicts that LC50 for fresh water species would to be 445.776 mg/l therefore that the substance is effectively not toxic to Daphnid.

 

Daphnid , 48-hr LC50 =   445.776 mg/L (ppm)

Daphnid, 30-day, ChV (chronic value) =43.190 mg/L (ppm)

Validity criteria fulfilled:
yes
Remarks:
US EPA accepted QSAR method for chemicals properties assessment.
Conclusions:
The ECOSAR QSAR model predicts that LC50 for fresh water species would to be 445.776 mg/l therefore that the substance is effectively not toxic to Daphnid.
Executive summary:

The ECOSAR QSAR model predicts that LC50 for fresh water species would to be 445.776 mg/l therefore that the substance is effectively not toxic to Daphnid.

 

 Daphnid , 48-hr LC50 = 445.776 mg/L (ppm)

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
(Q)SAR
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification
Justification for type of information:
QSAR prediction: US EPA accepted QSAR method for chemicals properties assessment.
Qualifier:
according to
Guideline:
other: QSAR model
Principles of method if other than guideline:
EPI Suite v 4.0/ECOSARv1.00
GLP compliance:
no
Remarks:
not applicable. QSAR model
Analytical monitoring:
not required
Vehicle:
no
Test organisms (species):
other: Mysid shrimp
Details on test organisms:
Scientific name: Americamysis bahia
Common name: Opossum shrimp, Mysid shrimp, Mysids
Test type:
other: QSAR model
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
not applicable. QSAR model
Test temperature:
not applicable. QSAR model
pH:
not applicable. QSAR model
Dissolved oxygen:
not applicable. QSAR model
Salinity:
not applicable. QSAR model
Nominal and measured concentrations:
not applicable. QSAR model
Details on test conditions:
100% Sodium ethyl xanthate (CAS No. 140-90-9)
SMILES : S=C([S-])OCC.[Na+]
CHEM : Carbonodithioic acid, o-ethyl ester, sodium salt
MOL FOR: C3 H5 O1 S2 Na1
MOL WT : 144.18
Log Kow: 0.87 (used)
Wat Sol: 1.79E+004 mg/L (WskowWin estimate)
Reference substance (positive control):
not required
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
2 090.966 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
The ECOSAR QSAR model predicts that LC50 for fresh water species cited would to be 2090.966 mg/l therefore that the substance is effectively not toxic to Mysid shrimp.
Reported statistics and error estimates:
Reaction mass of SEX

Overview of QSARs estimations onshort-term aquatic toxicity of  Reaction mass of SEX to Mysid shrimp   

Method

 

Results

Remarks

Reference

QSAR

estimate

Mysid Shrimp, 96 -hr, LC50= 2090.966 mg/L (ppm)

Mysid Shrimp (SW), 30-day, ChV (chronic value) = 252.828 mg/L (ppm)

 

 

Reliable with restrictions,

QSAR estimated data

ECOSAR Program (v1.00)

 

  

Mysid Shrimp, 96 -hr, LC50= 2090.966 mg/L (ppm)

Mysid Shrimp (SW), 30-day, ChV (chronic value) = 252.828 mg/L (ppm)

 

Validity criteria fulfilled:
yes
Remarks:
US EPA accepted QSAR method for chemicals properties assessment.
Conclusions:
The ECOSAR QSAR model predicts that LC50 for fresh water species cited would to be   2090.966 mg/l therefore that the substance is effectively not toxic to Mysid shrimp.
Executive summary:

The ECOSAR QSAR model predicts that LC50 for fresh water species cited would to be   2090.966 mg/l therefore that the substance is effectively not toxic to Mysid shrimp.

 

 

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Daphnia magna
Details on test organisms:
SPECIES #
(NAME): 5 Daphnia magna (Water Flea)
AGE: NR NR
LIFE STAGE: not reported, unknown
COMMENT: NR
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 h
Hardness:
NR
Test temperature:
20 °C
pH:
8.4
Dissolved oxygen:
O2-concentration was 9.8 mg/L,
conductivity was 325 µS/cm.
Salinity:
NR
Nominal and measured concentrations:
TYPE VALUE RANGES STANDARD CONC (ug/L) IONCIC FRACTION
F 350 NR TO NR 350 ug/L NR
Details on test conditions:
TEST CONDITIONS
STUDY TYPE:
MEDIA: FW (Fresh water)
LOCATION: LAB (Laboratory, indoor)
CONTROL: I (insufficient control)
EXPOSURE TYPE: S ( static)
APPLICATION FREQ.: 1 X
EXPOSURE DUR.: 24 hour(s)
STAND DUR. (D): 1 day(s
TEST CONCENTRATION
CHEM ANAL. METHOD: unmeasured
UNIT OF MEASURE: ug/L micrograms per liter

TYPE VALUE RANGES STANDARD CONC (ug/L) IONCIC FRACTION
F 350 NR TO NR 350 ug/L NR

WATER QUALITY VALUE RANGE (UNIT)
TEMPERATURE: 20 NR TO NR C
pH: 8.4 NR TO NR
HARDNESS: NR NR TO NR mg/L CaCO3
SALINITY: NR NR TO NR
ALKALINITY: NR NR TO NR mg/L CaCO3
CONDUCTIVITY: 325 NR TO NR µS/cm
DISSOLVED OXYGEN: 9.8 NR TO NR mg/l
ORGANIC CARBON (NR): NR NR TO NR NR
Reference substance (positive control):
no
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
0.35 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
LC10
Effect conc.:
0.18 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
other: EC95
Effect conc.:
0.85 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
A 24 h EC50 of 0.35 mg/L has been determined for the effects of the test substance on immobilation of the Daphnia magna .
EFFECT RESULTS
EFFECT:Intoxication
TREND:NR
RESPONSE SITE:NR
% EFFECT:NR
EFFECT MEASUREMENT:Immobile

ENDPOINT: Effective concentration to 50% of test organisms
ENDPOINT ASSIGN.:X
SIGNIFICANCE: NA
LEVEL:NA
BCF Value (F): NR ( NR to NR
OTHER EFFECTS: EC10-95, FATE
Validity criteria fulfilled:
yes
Conclusions:
A 24 h EC50 of 0.35 mg/L has been determined for the effects of the test substance on immobilation of the Daphnia magna .
Executive summary:

A 24 h EC50 of 0.35 mg/L has been determined for the effects of the test substance on immobilation of the Daphnia magna . The The

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
other: published data
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
Reaction mass of SEX are related compound to PEX
Qualifier:
equivalent or similar to
Guideline:
EPA OPPTS 850.1020 (Gammarid Acute Toxicity Test)
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Gammarus sp.
Details on test organisms:
SPECIES #
(NAME): 118 Gammarus sp. (Scud, Amphipod)
AGE: NR NR
LIFE STAGE: not reported, unknown
COMMENT: NR
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
118 to 125 mg/L CaCO3
Test temperature:
10 0C
pH:
7.9 to 8.2
Dissolved oxygen:
> 1 mg/L
Salinity:
NR
Nominal and measured concentrations:
TYPE VALUE RANGES STANDARD CONC (ug/L) IONCIC FRACTION
F 52000 NR TO NR 52000 ug/L NR
Details on test conditions:
TEST CONDITIONS
STUDY TYPE:
MEDIA: FW (Fresh water)
LOCATION: LAB (Laboratory, indoor)
CONTROL: I (insufficient control)
EXPOSURE TYPE: S ( static)
APPLICATION FREQ.: 1 X
EXPOSURE DUR.: 96 hour(s)
STAND DUR. (D): 4 day(s
TEST CONCENTRATION
CHEM ANAL. METHOD: unmeasured
UNIT OF MEASURE: ug/L micrograms per liter


TYPE VALUE RANGES STANDARD CONC (ug/L ) IONCIC FRACTION
F 52000 NR TO NR 52000 ug/L NR

WATER QUALITY VALUE RANGE (UNIT)
TEMPERATURE: 10 NR TO NR C
pH: NR 7.9 TO 8.2
HARDNESS: NR 118 TO 125 mg/L CaCO3
SALINITY: NR NR TO NR
ALKALINITY: NR NR TO NR NR
CONDUCTIVITY: NR NR TO NR NR
DISSOLVED OXYGEN: SAT NR TO NR mg/l
ORGANIC CARBON (NR): NR NR TO NR NR


Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
52 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
EFFECT RESULTS
EFFECT: Mortality
TREND: NR
RESPONSE SITE: NR
% EFFECT: NR
EFFECT MEASUREMENT:Mortality
ENDPOINT
ENDPOINT: Lethal concentration to 50% of test organisms
ENDPOINT ASSIGN.: X
SIGNIFICANCE: NA
LEVEL: NA
BCF Value (F): NR ( NR to NR)
Validity criteria fulfilled:
yes
Conclusions:
A 96 h LC50 of 52 mg/L has been determined for the effects of the test substance on mortality of the Gammarus sp. (Scud, Amphipod).
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
other: published data
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
The Reaction mass of SEX, the subject of this dossier is expected to exhibit very similar toxicity to sodium O-sec-butyl dithiocarbonate (CAS No. 36551-21-0)), which is also xanthate compound. Comparable metabolism would occur.
Qualifier:
equivalent or similar to
Guideline:
EPA OPPTS 850.1020 (Gammarid Acute Toxicity Test)
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Gammarus sp.
Details on test organisms:
SPECIES #
(NAME): 118 Gammarus sp. (Scud, Amphipod)
AGE: NR NR
LIFE STAGE: not reported, unknown
COMMENT: NR
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
118 to 125 mg/L CaCO3
Test temperature:
10 0C
pH:
7.9 to 8.2
Dissolved oxygen:
> 1 mg/L
Salinity:
NR
Nominal and measured concentrations:
TYPE VALUE RANGES STANDARD CONC (ug/L) IONCIC FRACTION
F 8500 NR TO NR 8500 ug/L NR
Details on test conditions:
TEST CONDITIONS
STUDY TYPE:
MEDIA: FW (Fresh water)
LOCATION: LAB (Laboratory, indoor)
CONTROL: I (insufficient control)
EXPOSURE TYPE: S ( static)
APPLICATION FREQ.: 1 X
EXPOSURE DUR.: 96 hour(s)
STAND DUR. (D): 4 day(s
TEST CONCENTRATION
CHEM ANAL. METHOD: unmeasured
UNIT OF MEASURE: ug/L micrograms per liter


TYPE VALUE RANGES STANDARD CONC (ug/L ) IONCIC FRACTION
F 8500 NR TO NR 8500 ug/L NR

WATER QUALITY VALUE RANGE (UNIT)
TEMPERATURE: 10 NR TO NR C
pH: NR 7.9 TO 8.2
HARDNESS: NR 118 TO 125 mg/L CaCO3
SALINITY: NR NR TO NR
ALKALINITY: NR NR TO NR NR
CONDUCTIVITY: NR NR TO NR NR
DISSOLVED OXYGEN: SAT NR TO NR mg/l
ORGANIC CARBON (NR): NR NR TO NR NR


Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
8.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
The Reaction mass of SEX, the subject of this dossier is expected to exhibit very similar toxicity to sodium O-sec-butyl dithiocarbonate (CAS No. 36551-21-0)), which is also xanthate compound. Comparable metabolism would occur.

EFFECT RESULTS
EFFECT: Mortality
TREND: NR
RESPONSE SITE: NR
% EFFECT: NR
EFFECT MEASUREMENT:Mortality
ENDPOINT
ENDPOINT: Lethal concentration to 50% of test organisms
ENDPOINT ASSIGN.: X
SIGNIFICANCE: NA
LEVEL: NA
BCF Value (F): NR ( NR to NR)
Validity criteria fulfilled:
yes
Conclusions:
A 96 h LC50 of 8.5 mg/L has been determined for the effects of the test substance on mortality of the Gammarus sp.
(Scud, Amphipod).
The Reaction mass of SEX, the subject of this dossier is expected to exhibit very similar toxicity to sodium O-sec-butyl dithiocarbonate (CAS No. 36551-21-0)), which is also xanthate compound. Comparable metabolism would occur.
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
other: published data
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
This paper focused mainly on the effects of xanthates on duckweed, and did not have a lot of details on the Daphnia bioassay. However, as some important experimental parameters were reported, the overall score isn’t too far from 70%, and a standard OECD method was used, this paper has been given a satisfactory reliability rating.
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Daphnia magna
Details on test organisms:
SPECIES #
(NAME): 5 Daphnia magna (Water Flea)
AGE: NR NR
LIFE STAGE: not reported, unknown
COMMENT: NR
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 h
Hardness:
NR
Test temperature:
20 °C
pH:
8.4
Dissolved oxygen:
O2-concentration was 9.8 mg/L,
conductivity was 325 µS/cm.
Salinity:
NR
Nominal and measured concentrations:
TYPE VALUE RANGES STANDARD CONC (ug/L) IONCIC FRACTION
F 3000 NR TO NR 3000 ug/L NR
Details on test conditions:
TEST CONDITIONS
STUDY TYPE:
MEDIA: FW (Fresh water)
LOCATION: LAB (Laboratory, indoor)
CONTROL: I (insufficient control)
EXPOSURE TYPE: S ( static)
APPLICATION FREQ.: 1 X
EXPOSURE DUR.: 24 hour(s)
STAND DUR. (D): 1 day(s
TEST CONCENTRATION
CHEM ANAL. METHOD: unmeasured
UNIT OF MEASURE: ug/L micrograms per liter

TYPE VALUE RANGES STANDARD CONC (ug/L) IONCIC FRACTION
F 3000 NR TO NR 3000 ug/L NR

WATER QUALITY VALUE RANGE (UNIT)
TEMPERATURE: 20 NR TO NR C
pH: 8.4 NR TO NR
HARDNESS: NR NR TO NR mg/L CaCO3
SALINITY: NR NR TO NR
ALKALINITY: NR NR TO NR mg/L CaCO3
CONDUCTIVITY: 325 NR TO NR µS/cm.
DISSOLVED OXYGEN: 9.8 NR TO NR mg/l
ORGANIC CARBON (NR): NR NR TO NR NR
Reference substance (positive control):
no
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC10
Effect conc.:
1.7 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
other: EC95
Effect conc.:
6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
TheReaction mass of SEX, the subject of this dossier is expected to exhibit very similar toxicity to Potassium amyl xanthate (CAS No. 2720-73-2)), which is also xanthate compound. Comparable metabolism would occur.
EFFECT RESULTS
EFFECT:Intoxication
TREND:NR
RESPONSE SITE:NR
% EFFECT:NR
EFFECT MEASUREMENT:Immobile

ENDPOINT: Effective concentration to 50% of test organisms
ENDPOINT ASSIGN.:X
SIGNIFICANCE: NA
LEVEL:NA
BCF Value (F): NR ( NR to NR
OTHER EFFECTS: EC10-95, FATE
Validity criteria fulfilled:
yes
Conclusions:
A 24 h EC50 of 3 mg/L has been determined for the effects of the test substance on immobilation of the Daphnia magna .
The Reaction mass of SEX the subject of this dossier is expected to exhibit very similar toxicity to Potassium amyl xanthate (CAS No. 2720-73-2)), which is also xanthate compound. Comparable metabolism would occur.
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
other: published data
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
This paper focused mainly on the effects of xanthates on duckweed, and did not have a lot of details on the Daphnia bioassay. However, as some important experimental parameters were reported, the overall score isn’t too far from 70%, and a standard OECD method was used, this paper has been given a satisfactory reliability rating.
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Daphnia magna
Details on test organisms:
SPECIES #
(NAME): 5 Daphnia magna (Water Flea)
AGE: NR NR
LIFE STAGE: not reported, unknown
COMMENT: NR
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 h
Hardness:
NR
Test temperature:
20 °C
pH:
8.4
Dissolved oxygen:
O2-concentration was 9.8 mg/L,
conductivity was 325 µS/cm.
Salinity:
NR
Nominal and measured concentrations:
TYPE VALUE RANGES STANDARD CONC (ug/L) IONCIC FRACTION
F 3700 NR TO NR 3700 ug/L NR
Details on test conditions:
TEST CONDITIONS
STUDY TYPE:
MEDIA: FW (Fresh water)
LOCATION: LAB (Laboratory, indoor)
CONTROL: I (insufficient control)
EXPOSURE TYPE: S ( static)
APPLICATION FREQ.: 1 X
EXPOSURE DUR.: 24 hour(s)
STAND DUR. (D): 1 day(s
TEST CONCENTRATION
CHEM ANAL. METHOD: unmeasured
UNIT OF MEASURE: ug/L micrograms per liter

TYPE VALUE RANGES STANDARD CONC (ug/L) IONCIC FRACTION
F 3700 NR TO NR 3700 ug/L NR

WATER QUALITY VALUE RANGE (UNIT)
TEMPERATURE: 20 NR TO NR C
pH: 8.4 NR TO NR
HARDNESS: NR NR TO NR mg/L CaCO3
SALINITY: NR NR TO NR
ALKALINITY: NR NR TO NR mg/L CaCO3
CONDUCTIVITY: 325 NR TO NR µS/cm.
DISSOLVED OXYGEN: 9.8 NR TO NR mg/l
ORGANIC CARBON (NR): NR NR TO NR NR
Reference substance (positive control):
no
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
3.7 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC10
Effect conc.:
1.9 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
other: EC95
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
The Reaction mass of SEX, the subject of this dossier is expected to exhibit very similar toxicity to Sodium isopropyl xanthate (CAS No. 140-93-2)), which is also xanthate compound. Comparable metabolism would occur.

EFFECT RESULTS
EFFECT:Intoxication
TREND:NR
RESPONSE SITE:NR
% EFFECT:NR
EFFECT MEASUREMENT:Immobile

ENDPOINT: Effective concentration to 50% of test organisms
ENDPOINT ASSIGN.:X
SIGNIFICANCE: NA
LEVEL:NA
BCF Value (F): NR ( NR to NR
OTHER EFFECTS: EC10-95, FATE
Validity criteria fulfilled:
yes
Conclusions:
A 24 h EC50 of 3.7 mg/L has been determined for the effects of the test substance on immobilation of the Daphnia magna.
The Reaction mass of SEX, the subject of this dossier is expected to exhibit very similar toxicity to Sodium isopropyl xanthate (CAS No. 140-93-2)), which is also xanthate compound. Comparable metabolism would occur.
Executive summary:

A 24 h EC50 of 3.7 mg/L has been determined for the effects of the test substance on immobilation of the Daphnia magna.

The Reaction mass of SEX, the subject of this dossier is expected to exhibit very similar toxicity to Sodium isopropyl xanthate (CAS No. 140-93-2)), which is also xanthate compound. Comparable metabolism would occur.

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
other: published data
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Justification for type of information:
Guideline study without detailed documentation. Incubation carried out in sealed vessels due to volatility of CS2.
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
yes
Remarks:
incubation in sealed vessels due to volatility of CS2
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
- Stock solution were prepared fresh each day.
- Test solutions were renewed daily.
Test organisms (species):
Daphnia magna
Details on test organisms:
SPECIES #
(NAME): 5 Daphnia magna
(Water Flea)
AGE: NR NR
LIFE STAGE: not reported, unknown
COMMENT: Food: Chlorella pyrenoidosa
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
225 mg CaCO3/L
Test temperature:
20 °C
pH:
8.1
Dissolved oxygen:
Not reported
Salinity:
Not reported
Nominal and measured concentrations:
TEST CONCENTRATION
CHEM ANAL. METHOD: unmeasured
UNIT OF MEASURE: ug/L micrograms per liter

TYPE VALUE RANGE STANDARD CONC (ug/L) ION
F 2100 1900 TO2200 2100 (1900-2200) ug/L NR
Details on test conditions:
Tests were carried out in sealed vessels to prevent evaporation of CS2.
The complete compostion of the test medium is given in Van Leeuwen and Maas, Environ. Pollution A37: 105-115 (1985).
Daphnids were fed with algal cells (Chlorella pyrenoidosa), 1E5 cells/mL.
Reference substance (positive control):
no
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
2.1 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mobility
Remarks on result:
other: 95% C.I.: 1.9 - 2.2 mg/L
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
3.98 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: On a molecular weight scaled basis, the EC50 would be 3.98 mg/l. (2.1 x 144.19) /76.13 =3.98 mg/l
Details on results:
The Reaction mass of SEX, the subject of this dossier is expected to exhibit very similar toxicity to Carbon disulphide (CS2, (CAS No. 75-15-0)), the decomposition product ofReaction mass of SEX. Comparable metabolism would occur.
On a molecular weight scaled basis, the EC50 would be 3.98 mg/l. (2.1 x 144.19) /76.13 =3.98 mg/l
Validity criteria fulfilled:
yes
Conclusions:
The 48-h EC50 for Daphnia magna in a sealed system was 2.1 mg /L.
The Reaction mass of SEX, the subject of this dossier is expected to exhibit very similar toxicity to Carbon disulphide (CS2, (CAS No. 75-15-0)), the decomposition product ofReaction mass of SEX. Comparable metabolism would occur.
On a molecular weight scaled basis, the EC50 would be 3.98 mg/l. (2.1 x 144.19) /76.13 =3.98 mg/l
Executive summary:

The Reaction mass of SEX, the subject of this dossier is expected to exhibit very similar toxicity to Carbon disulphide (CS2, (CAS No. 75-15-0)), the decomposition product ofReaction mass of SEX. Comparable metabolism would occur.

On a molecular weight scaled basis, the EC50 would be 3.98 mg/l. (2.1 x 144.19) /76.13 =3.98 mg/l

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
other: published data
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
This paper focused mainly on the effects of xanthates on duckweed, and did not have a lot of details on the Daphnia bioassay. However, as some important experimental parameters were reported, the overall score isn’t too far from 70%, and a standard OECD method was used, this paper has been given a satisfactory reliability rating.
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Daphnia magna
Details on test organisms:
SPECIES #
(NAME): 5 Daphnia magna (Water Flea)
AGE: NR NR
LIFE STAGE: not reported, unknown
COMMENT: NR
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 h
Hardness:
NR
Test temperature:
20 °C
pH:
8.4
Dissolved oxygen:
O2-concentration was 9.8 mg/L,
conductivity was 325 µS/cm.
Salinity:
NR
Nominal and measured concentrations:
TYPE VALUE RANGES STANDARD CONC (ug/L) IONCIC FRACTION
F 3600 NR TO NR 3600 ug/L NR
Details on test conditions:
TEST CONDITIONS
STUDY TYPE:
MEDIA: FW (Fresh water)
LOCATION: LAB (Laboratory, indoor)
CONTROL: I (insufficient control)
EXPOSURE TYPE: S ( static)
APPLICATION FREQ.: 1 X
EXPOSURE DUR.: 24 hour(s)
STAND DUR. (D): 1 day(s
TEST CONCENTRATION
CHEM ANAL. METHOD: unmeasured
UNIT OF MEASURE: ug/L micrograms per liter

TYPE VALUE RANGES STANDARD CONC (ug/L) IONCIC FRACTION
F 3600 NR TO NR 3600 ug/L NR

WATER QUALITY VALUE RANGE (UNIT)
TEMPERATURE: 20 NR TO NR C
pH: 8.4 NR TO NR
HARDNESS: NR NR TO NR mg/L CaCO3
SALINITY: NR NR TO NR
ALKALINITY: NR NR TO NR mg/L CaCO3
CONDUCTIVITY: 325 NR TO NR µS/cm.
DISSOLVED OXYGEN: 9.8 NR TO NR mg/l
ORGANIC CARBON (NR): NR NR TO NR NR
Reference substance (positive control):
no
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
3.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
3.01 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: On a molecular weight scaled basis, the EC50 would be 3.01 mg/l. (3.6 x 144.19) /172.24 =3.01 mg/l
Duration:
24 h
Dose descriptor:
EC10
Effect conc.:
1.8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
other: EC95
Effect conc.:
11 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
The Reaction mass of SEX the subject of this dossier is expected to exhibit very similar toxicity to Sodium isobutyl xanthate (CAS No. 25306-75-6),)), which is also xanthate compound. Comparable metabolism would occur.
On a molecular weight scaled basis, the EC50 would be 3.01 mg/l. (3.6 x 144.19) /172.24 =3.01 mg/l

EFFECT RESULTS
EFFECT:Intoxication
TREND:NR
RESPONSE SITE:NR
% EFFECT:NR
EFFECT MEASUREMENT:Immobile

ENDPOINT: Effective concentration to 50% of test organisms
ENDPOINT ASSIGN.:X
SIGNIFICANCE: NA
LEVEL:NA
BCF Value (F): NR ( NR to NR
OTHER EFFECTS: EC10-95, FATE
Validity criteria fulfilled:
yes
Conclusions:
A 24 h EC50 of 3.6 mg/L has been determined for the effects of the test substance on immobilation of the Daphnia magna .
The Reaction mass of SEX, the subject of this dossier is expected to exhibit very similar toxicity to Sodium isobutyl xanthate (CAS No. 25306-75-6),)), which is also xanthate compound. Comparable metabolism would occur.
On a molecular weight scaled basis, the EC50 would be 3.01 mg/l. (3.6 x 144.19) /172.24 =3.01 mg/l
Executive summary:

A 24 h EC50 of 3.6 mg/L has been determined for the effects of the test substance on immobilation of the Daphnia magna .

The Reaction mass of SEX, the subject of this dossier is expected to exhibit very similar toxicity to Sodium isobutyl xanthate (CAS No. 25306-75-6),)), which is also xanthate compound. Comparable metabolism would occur.

On a molecular weight scaled basis, the EC50 would be 3.01 mg/l. (3.6 x 144.19) /172.24 =3.01 mg/l

Description of key information

A 24 h EC50 of 0.35 mg/L has been determined for the effects of the Sodium ethyl xanthate as a main constituent of Reaction mass of ethanol and sodium O-ethyl dithiocarbonate and sodium hydroxide on immobilation of the Daphnia magna, under the conditions of the study of Xu,Y., J.P. Lay, and F. Korte,1988.
The ECOSAR QSAR model predicts that LC50 for fresh water species would to be 445.776 mg/l therefore that the substance is effectively not toxic to Daphnid. Daphnid , 48-hr LC50 = 445.776 mg/L (ppm)
The available data for marine water short-term to aquatic invertebrate are limited and to obtaine the value for PNECs for saltwaters is taken account a assesment factor 10 to extrapolate to LC50 following the “Guidance on Information Requirement and Chemical Safety Assessment R.10: Characterisation of dose [concentration]- response for environment”and “Table R.10-5 Assessment factors proposed for deriving PNEC water for saltwater for different data sets”, to account for the possibility that greater taxonomic diversity in the marine environment makes it likely that some marine taxa may be more sensitive than the most sensitive freshwater taxa.
48-hr LC50(Salt water) = 44.6 mg/L (ppm)

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
0.35 mg/L
EC50/LC50 for marine water invertebrates:
44.6 mg/L

Additional information

A 24 h EC50 of 0.35 mg/L has been determined for the effects of the Sodium ethyl xanthate as a main constituent of Reaction mass of ethanol and sodium O-ethyl dithiocarbonate and sodium hydroxide on immobilation of the Daphnia magna, under the conditions of the study of Xu,Y., J.P. Lay, and F. Korte,1988.

The ECOSAR QSAR model predicts that LC50 for fresh water species would to be 445.776 mg/l therefore that the substance is effectively not toxic to Daphnid. Daphnid , 48-hr LC50 = 445.776 mg/L (ppm)

The available data for marine water short-term to aquatic invertebrate are limited and to obtaine the value for PNECs for saltwaters is taken account a assesment factor 10 to extrapolate to LC50 following the “Guidance on Information Requirement and Chemical Safety Assessment R.10: Characterisation of dose [concentration]- response for environment”and “Table R.10-5 Assessment factors proposed for deriving PNEC water for saltwater for different data sets”, to account for the possibility that greater taxonomic diversity in the marine environment makes it likely that some marine taxa may be more sensitive than the most sensitive freshwater taxa.

 

48-hr LC50(Salt water) = 44.6 mg/L (ppm)